Persistent neutrophil activation in mild asthma

Kallenbach, J; Baynes, Roy D.; Fine, Barbara; Dajee, D.; Bezwoda, W. R.

doi:10.1016/0091-6749(92)90084-f

Cited by 23 publications

(10 citation statements)

References 6 publications

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“…This would suggest that PMNs have more important deleterious effects in blood than in the lung tissues. Moreover, KELLENBACH et al [34] and others have also shown a fast PMN recruitment following exercise allergen challenge after bronchoprovocation in asthma.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Vachier

Chanez²,

Doucen³

et al. 1994

Eur Respir J

107

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E En nh ha an nc ce em me en nt t o of f r re ea ac ct ti iv ve e o ox xy yg ge en n s sp pe ec ci ie es s f fo or rm ma at ti io on n i in n s st ta ab bl le e a an nd d u un ns st ta ab bl le e a as st th hm ma at ti ic c p pa at ti ie en nt ts s ABSTRACT: There is increasing evidence to suggest that human blood polymorphonuclear neutrophils (PMNs) and monocytes play an important role in the inflammatory processes of asthma. In asthmatic patients, PMNs and monocytes were shown to be activated more than in healthy subjects. We investigated the capacity of these two cell populations to generate reactive oxygen species (ROS) in stable and unstable asthmatic patients. The two populations of asthmatic patients were identified by asthma activity, as expressed by clinical events occurring within 2 weeks prior to the study. Oxygen species formation was analysed for isolated purified PMNs and monocytes (Mos) by chemiluminescence (CL) using lucigenin and luminol as luminescent probes. CL was determined on nonstimulated and on phorbol myristate acetate (PMA)-stimulated cells. The stimulatability coefficient (PMA-stimulated/nonstimulated cell ratio) of each cell population was then calculated.Resting PMNs and Mos generated significantly greater amounts of ROS in stable asthmatic patients, and much more in unstable asthmatic patients, as compared to healthy subjects, both in lucigenin and luminol enhanced CL. Non O 2 · -ROS production from PMA-stimulated PMNs and Mos was identical in unstable asthmatic patients and in healthy subjects, whereas a significant decrease was observed in stable asthmatic patients, as assessed by luminol enhanced CL. PMA-stimulated cells showed no difference in O 2 · -generation, as assessed by lucigenin enhanced CL. However, the stimulatability coefficient of all asthmatic patients was always significantly lower than that of healthy subjects.These results suggest that there are differences in priming and stimulation of Ros production from PMNs and Mos between stable and unstable asthmatic patients. Release of oxygen species from these cells may be implicated in the pathophysiology of unstable asthma.

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Section: Discussionmentioning

confidence: 99%

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Vachier

Chanez²,

Doucen³

et al. 1994

Eur Respir J

107

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“…The mean osmolality of saliva is 95±12 mOsm·kg -1 H 2 O, and this clearly indicates that there was no contamination with saliva in the samples analysed. As demonstrated in our previous study, dissolved CO 2 and HCO 3 -are mainly responsible for the relatively high osmolality of expired breath condensate [13].…”

Section: Measurement Of Tbar Productsmentioning

confidence: 98%

“…When activated, these cells release mediators and cytokines that amplify the inflammatory process, including release of reactive oxygen species, superoxide radicals (O 2 · -) and hydrogen peroxide (H 2 O 2 ) [2,3]. Oxidative damage to the epithelium produces hyperresposiveness of human peripheral airways [4,5].…”

mentioning

confidence: 99%

Increased hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients

Antczak¹,

Nowak²,

Shariati³

et al. 1997

Eur Respir J

177

120

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Symptoms of bronchial asthma are a manifestation of airway inflammation. Circulatory leucocytes (predominantly eosinophils, mast cells and neutrophils), release inflammatory mediators, including reactive oxygen species, i.e. superoxide anion which is dismutated to hydrogen peroxide (H 2 O 2 ). Neutrophils from asthmatics generate greater amounts of these species than those of healthy subjects. Some of the H 2 O 2 and thiobarbituric acid-reactive products (TBARs) can evaporate from alveolar lining fluid, and could be expired from the airways of asthmatics. In this study, therefore, we determined whether asthmatic patients exhale more H 2 O 2 and TBARs than healthy subjects.We examined 10 healthy subjects as a control group and 21 asthmatic subjects. In asthmatic subjects, forced expiratory volume in one second (FEV1), was 68±9% of predicted value, peak expiratory flow rate (PEFR) was 65±8% pred, and bronchial reversibility was 34±5% of prebronchodilated FEV1. The mean H 2 O 2 level measured spectrofluorimetrically in the expired breath condensate of asthmatic subjects was 26 fold higher than that in healthy controls (0.26±0.29 vs 0.01±0.03 nM; p<0.05). The concentration of TBARs in breath condensate was also higher in asthmatic patients compared with nonasthmatics (0.073±0.071 vs 0.004±0.009 nM; p<0.05). There was a significant correlation between H 2 O 2 level and concentration of TBARs in asthmatic patients (r=0.74; p<0.01). There was also a strong inverse correlation between H 2 O 2 content of all asthmatics and FEV1% pred (r= -0.63; p<0.005) and PEFR% pred (r= -0.52; p<0.05).We conclude that there are elevated levels of hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients, and that measurement of these substances in the expired breath condensate could be a simple, noninvasive method that could be used as a biochemical marker of airway inflammation. Eur Respir J 1997; 10: 1235-1241

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“…Our results can be explained by the earlier systemic activation of peripheral blood phagocytes (neutrophils) still in the acute phase of seasonal allergic rhinoconjunctivitis prior/during pregnancy. Indeed, it was already described that a great amount of various infl ammatory mediators could be released during the active period of an allergic infl ammation even from a small area of the body (like rhinoconjunctivitis) causing a generalized and long-lasting activation of neutrophils (KALLENBACH et al, 1992;SZABÓ et al, 2000). LF released by activated neutrophils may mediate numerous compensatory effects to inhibit allergic infl ammations, decreasing mast cell degranulation, tryptase activity, eosinophils' migration, and promoting the Th1-type immune responses (ELROD et al, 1997;HE et al 2003;DE LA ROSA et al, 2008;BOURNAZOU et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

A Possible Role of Elevated Breast Milk Lactoferrin and the Cytokine IL-17 Levels in Predicting Early Allergy in Infants: A Pilot Study

et al. 2016

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In this study, we examined the relationship between levels of lactoferrin (LF) and IL-17 in human serum and breast milk and the development of allergy in children. LF and IL-17 levels were determined by ELISA in healthy (n=19) and allergic mothers (n=21) on the 5 th day after delivery. Two years later, information on breastfeeding and allergic outcomes was collected by questionnaires from parents of both groups and district child care nurses. Signifi cantly higher concentrations of LF were found in the breast milk of allergic mothers compared to the healthy controls. At 2 years of age, only those three infants became allergic from the atopic group in whose starting breast milk samples a very high LF level (306 μg mg -1 protein) or simultaneously elevated concentrations of LF and IL-17 were measured. These fi ndings indicate that the very early measurement of LF and IL-17 levels in the breast milk of allergic mothers may help to predict the allergy development in their infants.

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Persistent neutrophil activation in mild asthma

Cited by 23 publications

References 6 publications

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Increased hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients

A Possible Role of Elevated Breast Milk Lactoferrin and the Cytokine IL-17 Levels in Predicting Early Allergy in Infants: A Pilot Study

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