Persistent luminescence nanorods-based autofluorescence-free biosensor for prostate-specific antigen detection

Yin, Zipeng; Zhu, Ling; Lv, Zijian; Li, Meijin; Tang, Dianping

doi:10.1016/j.talanta.2021.122563

Cited by 49 publications

(20 citation statements)

References 44 publications

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“…The detection of cancer biomarkers in liquid biopsy samples such as blood, urine, and saliva [ 9 , 10 , 11 ] is a rapid, non-invasive procedure for early diagnosis without requiring a tumor location [ 12 ]. There has been considerable progress in identifying biomarkers and fabricating biosensors, mostly using conventional 2D manufacturing processes [ 13 , 14 ]. Major biosensors for cancer diagnostic purposes are based on tumor markers, which indicate not just the presence of the disease but also the real-time situation of tumors.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is relevant to enhance the sensitivity and selectivity of the detection methods. For instance, Yin and co-workers assembled an autofluorescence-free biosensor with high analytical performance on prostate-specific antigen (PSA) detection [ 14 ] under optimal conditions. PSA is a biomarker used as an indicator for prostate cancer [ 15 ], but it is found at low concentrations in human serum, which demands biosensors with high specificity and sensitivity for early diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

et al. 2022

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Cancer is the second leading cause of death globally and early diagnosis is the best strategy to reduce mortality risk. Biosensors to detect cancer biomarkers are based on various principles of detection, including electrochemical, optical, electrical, and mechanical measurements. Despite the advances in the identification of biomarkers and the conventional 2D manufacturing processes, detection methods for cancers still require improvements in terms of selectivity and sensitivity, especially for point-of-care diagnosis. Three-dimensional printing may offer the features to produce complex geometries in the design of high-precision, low-cost sensors. Three-dimensional printing, also known as additive manufacturing, allows for the production of sensitive, user-friendly, and semi-automated sensors, whose composition, geometry, and functionality can be controlled. This paper reviews the recent use of 3D printing in biosensors for cancer diagnosis, highlighting the main advantages and advances achieved with this technology. Additionally, the challenges in 3D printing technology for the mass production of high-performance biosensors for cancer diagnosis are addressed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

et al. 2022

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“…Isothermal enzyme-free DNA amplification circuits have aroused enormous interest in the detection of biomarkers by virtue of their excellent signal amplification efficiency, such as hybridization chain assembly (HCA), − entropy-driven catalysis (EDC), − catalytic DNA assembly (CDA), − and deoxyribozyme-induced hydrolytic cleavage (DNAzyme). − Especially, the HCA amplifier presents less signal leakage for sensing purposes and has attracted extensive attention. In a typical HCA circuit, the target could activate the assembly of the hairpins to form long dsDNA polymers via toehold-mediated strand displacement. − The HCA circuits are particularly suitable for in situ visualization of biomarkers because the as-obtained DNA polymers tethering to initiators could efficiently prevent diffusion of the amplified output signals away from the target sites. To date, a lot of methods based on HCA circuits have been successfully applied in the detection of PNK in vitro .…”

Section: Introductionmentioning

confidence: 99%

An Autocatalytic DNA Circuit Based on Hybridization Chain Assembly for Intracellular Imaging of Polynucleotide Kinase

Wang

Chen

Wan

et al. 2022

ACS Appl. Mater. Interfaces

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Polynucleotide kinase (PNK) plays an essential role in various cellular events by regulating phosphorylation processes, and abnormal homeostasis of PNK could cause many human diseases. Herein, we proposed an autocatalytic hybridization system (AHS) through the elaborate integration of hybridization chain assembly (HCA) and catalytic DNA assembly (CDA) that enables a highly efficient positive feedback amplification. The PNK-targeting AHS biosensor is composed of three modules: a recognition module, an HCA amplification module, and a CDA autocatalytic module. In the presence of PNK, the recognition module could transform the PNK input into an exposed nucleic acid initiator (I). Then the initiator strand I could trigger the autonomous HCA process in the amplification module, and the resulted HCA products could reassemble the split CDA trigger strand T, subsequently inducing the CDA process in the autocatalytic module to form abundant DNA duplex products. Consequently, the embedded initiator strand I was liberated from the CDA duplex product to autonomously trigger the new rounds of HCA circuit. The rational integration and cooperative crossactivation between the HCA and CDA module could prominently accelerate the reaction and realize the exponential amplification efficiency by initiator regeneration. As a result, the self-sustainable AHS amplifier could implement the sensitive detection of PNK in vitro and in biological samples and further fulfill accurate monitoring of the intracellular PNK activity and the effective screening of PNK inhibitors. This work paves a way for exploiting highly efficient artificial DNA circuits to analyze low-abundance biomarkers, holding great potential in biochemical research and clinical diagnosis.

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“…Prostate-specific antigen (PSA) is an androgen-regulated serine protease, which is a reliable and preferred serum biomarker for the preoperative diagnosis and screening of early-stage prostate cancer [1][2]. This glycoprotein biomarker (33)(34) is also called human glandular kallikrein-3 (KLK3) and can be used to evaluate healthy people or patients (prostate cancer or other prostate diseases), where the normal serum concentration should be lower than 4 ng/mL (cutoff-value) for clinical screening [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Dopamine‐loaded Liposomes‐amplified Electrochemical Immunoassay Based on MXene (Ti₃C₂)−AuNPs

Liu

Tang

2022

Electroanalysis

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A simple and novel electrochemical immunoassay based on MXene (Ti3C2)−Au nanoparticles (AuNPs) was designed for sensitive screening of a disease‐related biomarker, prostate‐specific antigen (PSA), by using dopamine‐loaded liposomes (DLL) for signal amplification. The system involves two parts, namely, sandwich‐type immunoreaction to capture DLL and electrochemical measurement of dopamine. The target PSA can cause a specific antigen‐antibody reaction and DLL are enriched in the enzyme‐labeled pores. After Triton X‐100 is injected into the detection cell, the carried DLL was quickly cracked to release dopamine wrapped in the cavity. A nanocomposite consisting of MXene (Ti3C2) support to immobilize Au nanoparticles (Ti3C2−Au) was utilized to modify a glassy carbon electrode, which gives a strongly enhanced differential pulse voltammetric (DPV) signals for dopamine. In this case, the change of DPV signal depends on the amount of dopamine released by liposomes, which is further positively correlated with the concentration of the analyte PSA. Combining the of MXene (Ti3C2)−AuNPs nanomaterials (large specific surface area, excellent electrical conductivity, and good electrocatalytic properties) with the liposome signal amplification strategy, the electrochemical immunoassay exhibited excellent performance toward PSA determination with a broad linear range of 1 pg/mL to 50 ng/mL and limit of detection down to 0.31 pg/mL (S/N=3) under the optimized testing conditions. High specificity for PSA over other disease‐related biomarkers and acceptable nanocomposite/electrode stability were acquired. The excellent analytical performance shows that the current strategy provides an effective detection platform for clinical sample analysis.

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Persistent luminescence nanorods-based autofluorescence-free biosensor for prostate-specific antigen detection

Cited by 49 publications

References 44 publications

Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

An Autocatalytic DNA Circuit Based on Hybridization Chain Assembly for Intracellular Imaging of Polynucleotide Kinase

Dopamine‐loaded Liposomes‐amplified Electrochemical Immunoassay Based on MXene (Ti₃C₂)−AuNPs

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Persistent luminescence nanorods-based autofluorescence-free biosensor for prostate-specific antigen detection

Cited by 49 publications

References 44 publications

Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

Three-Dimensional Printing and Its Potential to Develop Sensors for Cancer with Improved Performance

An Autocatalytic DNA Circuit Based on Hybridization Chain Assembly for Intracellular Imaging of Polynucleotide Kinase

Dopamine‐loaded Liposomes‐amplified Electrochemical Immunoassay Based on MXene (Ti3C2)−AuNPs

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Dopamine‐loaded Liposomes‐amplified Electrochemical Immunoassay Based on MXene (Ti₃C₂)−AuNPs