Persistent epithelial barrier alterations in a rat model of postinfectious gut dysfunction

Fernández-Blanco, Joan Antoni; Barbosa, Silas Pereira; Medina, Fermín Sánchez de; Martı́nez, Vicente; Vergara, P.

doi:10.1111/j.1365-2982.2011.01777.x

Cited by 19 publications

(39 citation statements)

References 32 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During this time, animals were regularly monitored for clinical signs and body weight changes. Normal course of the infection was confirmed by a significant decrease of body weight after T. spiralis infection compared with controls, with a peak reduction on day 9 and a subsequent linear increase over time, as previously described by us (10,43).…”

Section: T Spiralis Infectionsupporting

confidence: 78%

“…Immunodetection of rMCP-2 was done on jejunal sections following standard immunohistochemical procedures using a monoclonal antibody (MS-RM4, 1:500; Moredun Animal Health, Edinburgh, UK), as previously described by us (10).…”

Section: Immunohistochemistry For Rmcp-2 and Rmcp-6 And MC Countsmentioning

confidence: 99%

“…Electrophysiological parameters were measured in jejunal segments stripped of the outer muscle layers and myenteric plexus in Ussing chambers, as previously described by us (10). Tissues were bathed bilaterally with 5 ml of 37°C oxygenated Krebs buffer.…”

Section: Ussing Chamber Studiesmentioning

confidence: 99%

“…Paracellular permeability was assessed by measuring mucosal to basolateral flux of fluorescein isothiocyanate-labeled dextran with an average molecular weight of 4 kDa (FD4; Sigma Aldrich, St. Louis, MO) as previously described (10). After stabilization, FD4 was added to the mucosal reservoir to a final concentration of 2.5 ϫ 10 Ϫ4 M. Basolateral samples (250 l, replaced by 250 l of appropriate buffer solution) were taken for subsequent fluorescence measurement against a standard curve.…”

Section: Ussing Chamber Studiesmentioning

confidence: 99%

“…For this purpose, we used Trichinella spiralis (T. spiralis)-infected rats, a validated model of MCassociated enteritis and gut dysfunction that is regarded as a valid model of IBS (7,10,35,43). We studied how intestinal function was affected under basal conditions and after PAR-2 activation in the early and the late postinfectious phases.…”

mentioning

confidence: 99%

See 4 more Smart Citations

PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat

Fernandez-Blanco¹,

Hollenberg

Martínez

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

Fernández-Blanco JA, Hollenberg MD, Martínez V, Vergara P. PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat. Am J Physiol Gastrointest Liver Physiol 304: G390 -G400, 2013. First published December 13, 2012; doi:10.1152/ajpgi.00387.2012.-Proteinase-activated receptor-2 (PAR-2) and mast cell (MC) mediators contribute to inflammatory and functional gastrointestinal disorders. We aimed to characterize jejunal PAR-2-mediated responses and the potential MC involvement in the early and late phases of a rat model of postinfectious gut dysfunction. Jejunal tissues of control and Trichinella spiralis-infected (14 and 30 days postinfection) rats, treated or not with the MC stabilizer, ketotifen, were used. Histopathology and immunostaining were used to characterize inflammation, PAR-2 expression, and mucosal and connective tissue MCs. Epithelial barrier function (hydroelectrolytic transport and permeability) and motility were assessed in vitro in basal conditions and after PAR-2 activation. Intestinal inflammation on day 14 postinfection (early phase) was significantly resolved by day 30 (late phase) although MC counts and epithelial permeability remained increased. PAR-2-mediated ion transport (Ussing chambers, in vitro) and epithelial surface PAR-2 expression were reduced in the early phase, with a trend toward normalization during the late phase. In control conditions, PAR-2 activation (organ bath) induced biphasic motor responses (relaxation followed by excitation). At 14 days postinfection, spontaneous contractility and PAR-2-mediated relaxations were enhanced; motor responses were normalized on day 30. Postinfectious changes in PAR-2 functions were not affected by ketotifen treatment. We concluded that, in the rat model of Trichinella spiralis infection, alterations of intestinal PAR-2 function and expression depend on the inflammatory phase considered. A lack of a ketotifen effect suggests no interplay between MCs and PAR-2-mediated motility and ion transport alterations. These observations question the role of MC mediators in PAR-2-modulating postinfectious gut dysfunction.

show abstract

Section: T Spiralis Infectionsupporting

confidence: 78%

Section: Immunohistochemistry For Rmcp-2 and Rmcp-6 And MC Countsmentioning

confidence: 99%

Section: Ussing Chamber Studiesmentioning

confidence: 99%

Section: Ussing Chamber Studiesmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat

Fernandez-Blanco¹,

Hollenberg

Martínez

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

show abstract

Impact of dextran sulphate sodium-induced colitis on the intestinal transport of the colon carcinogen PhIP

et al. 2015

View full text Add to dashboard Cite

Colorectal cancer is one of the most frequent cancers in Western countries. Chronic intestinal diseases such as Crohn's disease and ulcerative colitis, in which the intestinal barrier is massively disturbed, significantly raise the risk of developing a colorectal tumour. 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a genotoxic heterocyclic aromatic amine that is formed after strongly heating fish and meat. In this study, the hypothesis that PhIP uptake in the gut is increased during chronic colitis was tested. Chronic colitis was induced by oral administration of dextran sulphate sodium (DSS) to Fischer 344 rats. The transport of PhIP in eight different rat intestinal segments was examined in Ussing chambers. The tissues were incubated with 10 µM PhIP for 90 min, and the concentration of PhIP was determined in the mucosal and serosal compartments of the Ussing chambers as well as in the clamped tissues by LC-MS. Although chronic colitis was clearly induced in the rats, no differences in the intestinal transport of PhIP were observed between control and DSS-treated animals. The hypothesis that in the course of chronic colitis more PhIP is taken up by the intestinal epithelium, thereby increasing the risk of developing colorectal cancer, could not be confirmed in the present report.

show abstract

The different infectivity of Trichinella spiralis and Trichinella nativa in rat does not solely localize to enteral or parenteral phase

et al. 2012

View full text Add to dashboard Cite

Rats are selective hosts for Trichinella nativa; infection levels in their muscles remain low when compared to Trichinella spiralis. To identify in which phase of the life cycle the selective responses take place, rats were infected perorally (p.o.) and intravenously (i.v.) with T. spiralis and T. nativa. Six rats were placed in each group; three rats served as uninfected controls. The infection intensities were examined 5 or 6 weeks after the infection. T. spiralis muscle larva burden was 57 times higher compared to that of T. nativa after p.o. inoculation (mean larvae per gram ± SD, 1,243 ± 378.9 vs. 22 ± 21.5). In i.v. inoculation, 12 times more T. spiralis larvae reached the muscle than with T. nativa. Also, in vitro newborn larva (NBL) production at day 6 postinfection was analyzed for parasites originating from both rat and mouse. The mean number of in vitro-produced NBL of rat-origin T. spiralis during 24 h was 70 (95 % CI, 65-75) and of T. nativa, 23 (95 % CI, 21-24). Intriguingly, this difference was less obvious with mouse-origin female Trichinella. NBL production of mouse origin T. spiralis was 57 (95 % CI, 51-62) and T. nativa, 29 (95 % CI, 26-31). Intestine histology at day 40 post p.o. infection, serological response, and weight dynamics were indistinguishable between the Trichinella species but differed from controls. Our results show that the defense against T. nativa is not solely enteral in rats but may partly be explained by different reproduction processes for the two Trichinella species in rat.

show abstract

Persistent epithelial barrier alterations in a rat model of postinfectious gut dysfunction

Cited by 19 publications

References 32 publications

PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat

PAR-2-mediated control of barrier function and motility differs between early and late phases of postinfectious gut dysfunction in the rat

Impact of dextran sulphate sodium-induced colitis on the intestinal transport of the colon carcinogen PhIP

The different infectivity of Trichinella spiralis and Trichinella nativa in rat does not solely localize to enteral or parenteral phase

Contact Info

Product

Resources

About