Persistent Cyfip1 expression is required to maintain the adult subventricular zone neurogenic niche

Abstract

Neural stem cells (NSCs) persist throughout life in the subventricular zone (SVZ) niche of the lateral ventricles as B1 cells. Maintaining this population of NSCs depends on the balance between quiescence and self-renewing or self-depleting proliferation. Interactions between B1 cells and the surrounding niche are important in regulating this balance, but the mechanisms governing these processes have not been fully elucidated in adult mammals. The cytoplasmic FMRP-interacting protein (CYFIP1) regulates apical-basal polarity in the embryonic brain. Loss of Cyfip1 during embryonic development in mice disrupts the embryonic niche and affects cortical neurogenesis.However, a direct role for Cyfip1 in the regulation of adult NSCs has not been established. Here, we demonstrate that Cyfip1 expression is preferentially localized to B1 cells in the adult SVZ. Loss of Cyfip1 in the embryonic mouse brain results in altered adult SVZ architecture and expansion of the adult B1 cell population at the ventricular surface. Furthermore, acute deletion of Cyfip1 in adult NSCs results in a rapid change in adherens junction proteins as well as increased proliferation and the number of B1 cells at the ventricular surface. Together, these data indicate that CYFIP1 plays a critical role in the formation and maintenance of the adult SVZ niche and, furthermore, deletion of Cyfip1 unleashes the capacity of adult B1 cells for symmetric renewal to increase the adult NSC pool. SIGNIFICANCENeural stem cells (NSCs) persist in the subventricular zone (SVZ) of the lateral ventricles in adult mammals and their population is determined by the balance between quiescence and self-depleting or renewing cell division. The mechanisms regulating their biology are not fully understood. This study establishes that the cytoplasmic FMRP interacting protein 1 (Cyfip1) regulates NSC fate decisions in the adult SVZ and NSCs that are quiescent or typically undergo self-depleting divisions retain the ability to self-renew in the adult. This contributes to our understanding of how adult NSCs are regulated throughout life and has potential implications for human brain disorders.3

Las células troncales neuronales entéricas funcionalmente viables como alternativa para la reparación de la SNpc en un modelo de rata lesionada con 6-OHDA

“…En varias especies, durante la etapa postnatal y a lo largo de toda la vida, se ha demostrado que continúan generándose nuevas células. Las áreas neurogénicas incluyen a la zona subventricular (ZSV) (Habela et al, 2020), el bulbo olfatorio (BO) (Lim y Alvarez-Buylla, 2016) y el hipocampo (Kempermann et al, 2015;Pallotto y Deprez, 2014). Sin embargo, existe evidencia de que el estriado (Dayer et al, 2005) e incluso la SNpc (Zhao et al, 2003) podrían ser zonas neurogénicas.…”