Persistent, circulative transmission of begomoviruses by whitefly vectors

Rosen, Ran; Kanakala, Surapathrudu; Kliot, Adi; Pakkianathan, Britto Cathrin; Farich, Basheer Abu; Santana-Magal, Nadine; Elimelech, Meytar; Kontsedalov, Svetlana; Lebedev, Galina; Cilia, Michelle; Ghanim, Murad

doi:10.1016/j.coviro.2015.06.008

Cited by 146 publications

(124 citation statements)

References 76 publications

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“…Successful infection cycle for whitefly-transmitted begomoviruses depends on the efficiency of acquisition, transmission, and retention of the virus in the whitefly vector (Rosen et al, 2015). Although these interactions have been studied for many years, very little is known about the molecular mechanisms that govern the transmission of this group of viruses by their hemipteran vectors.…”

Section: Discussionmentioning

confidence: 99%

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

Kanakala

Ghanim

2016

Front. Plant Sci.

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Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous studies have shown that the transmission, retention, and circulation of TYLCV in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a protective role in response to the presence of the virus in the insect body. However, only a low number of such proteins have been identified. Here, the role of B. tabaci Cyclophilin B (CypB) in the transmission of TYLCV protein was investigated. Cyclophilins are a large family of cellular prolyl isomerases that have many molecular roles including facilitating protein-protein interactions in the cell. One cyclophilin protein has been implicated in aphid-luteovirus interactions. We demonstrate that the expression of CypB from B. tabaci is altered upon TYLCV acquisition and retention. Further experiments used immunocapture-PCR and co-immunolocalization and demonstrated a specific interaction and colocalization between CypB and TYLCV in the the midgut, eggs, and salivary glands. Membrane feeding of anti-CypB antibodies and TYLCV-infected plants showed a decrease in TYLCV transmission, suggesting a critical role that CypB plays in TYLCV transmission. Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A showed decrease in CypB-TYLCV colocalization in the midgut and virus transmission. Altogether, our results indicate that CypB plays an important role in TYLCV transmission by B. tabaci.

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Section: Discussionmentioning

confidence: 99%

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

Kanakala

Ghanim

2016

Front. Plant Sci.

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“…Begomoviruses are transmitted by the whitefly Bemisia tabaci in a persistent manner (Rosen et al, 2015). In October 2015, severe symptoms not previously reported by growers in the horticultural area of the Province of Trapani (Sicily, Italy) were observed on zucchini squash (Cucurbita pepo) in open fields.…”

Section: Figurementioning

confidence: 99%

First report of Tomato leaf curl New Delhi virus affecting zucchini squash in an important horticultural area of southern Italy

Panno¹,

Iacono²,

Davino³

et al. 2016

New Disease Reports

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“…Begomoviruses contain the largest known genus of ∼288 species of plant viruses and are generally known to be transmitted by the whitefly Bemisia tabaci in a circulative-nonpropagative manner (14,15). The whitefly B. tabaci is now recognized as a complex containing at least 35 cryptic species (16,17).…”

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confidence: 99%

Vector development and vitellogenin determine the transovarial transmission of begomoviruses

Wei

Guo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

113

114

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The majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. Begomoviruses are transmitted by the whitefly Bemisia tabaci in a circulative manner and are maintained through a plantinsect-plant cycle. Other routes of begomovirus transmission are not clearly known. Here, we report that transovarial transmission from female whiteflies to offspring often happens for one begomovirus, Tomato yellow leaf curl virus (TYLCV), and may have contributed significantly to its global spread. We found that TYLCV entry of the reproductive organ of its vector mainly depended on the developmental stage of the whitefly ovary, and the transovarial transmission of TYLCV to offspring increased with whitefly adult age. The specific interaction between virus coat protein (CP) and whitefly vitellogenin (Vg) was vital for virus entry into whitefly ovary. When knocking down the expression of Vg, the entry of TYLCV into ovary was inhibited and the transovarial transmission efficiency decreased. In contrast, another begomovirus, Papaya leaf curl China virus (PaLCuCNV), CP did not interact with whitefly Vg, and PaLCuCNV could not be transovarially transmitted by whiteflies. We further showed that TYLCV could be maintained for at least two generations in the absence of virusinfected plants, and the adult progenies were able to infect healthy plants in both the laboratory and field. This study reports the transovarial transmission mechanism of begomoviruses, and it may help to explain the evolution and global spread of some begomoviruses.begomovirus | transovarial transmission | vector development | vitellogenin | whitefly

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Persistent, circulative transmission of begomoviruses by whitefly vectors

Cited by 146 publications

References 76 publications

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

Implication of the Whitefly Bemisia tabaci Cyclophilin B Protein in the Transmission of Tomato yellow leaf curl virus

First report of Tomato leaf curl New Delhi virus affecting zucchini squash in an important horticultural area of southern Italy

Vector development and vitellogenin determine the transovarial transmission of begomoviruses

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