Persistence of the developmental block of in vitro fertilized domestic cat embryos to temporal variations in culture conditions

Swanson, William F.; Roth, Terri L.; Godke, R.A.

doi:10.1002/(sici)1098-2795(199603)43:3<298::aid-mrd3>3.0.co;2-l

Cited by 36 publications

(31 citation statements)

References 37 publications

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“…Techniques such as zona piercing [12] and intracytoplasmic sperm injection [13,14] can be used to circumvent fertilization barriers of sub-optimal ejaculated or rescued sperm. Domestic cat embryos can be readily generated by IVF using oocytes recovered from ovarian follicles and matured in vitro [15][16][17][18][19][20]. In contrast, oocytes from the excised ovaries of wild felid counterparts generally experience lower in vitro maturation/fertilization success [21] possibly due to the age and health status of the donors.…”

Section: Introductionmentioning

confidence: 99%

Improved felid embryo development by group culture is maintained with heterospecific companions

Spindler

Crichton²,

Ağca

et al. 2006

Theriogenology

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Section: Introductionmentioning

confidence: 99%

Improved felid embryo development by group culture is maintained with heterospecific companions

Spindler

Crichton²,

Ağca

et al. 2006

Theriogenology

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“…vitro embryo development [1][2][3][4][5][6][7][8][9]. In previous studies, we used TCM-199 [10] as a culture medium for cloned cat embryos.…”

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confidence: 99%

Effect of Culture Medium and Prostaglandin I2 (PGI2) Analogue on In Vitro Development of Parthenogenetically Activated Cat Oocytes

Yin

Lee

Jeon

et al. 2007

Journal of Reproduction and Development

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Abstract.A method of reliably producing developmentally competent cat embryos in vitro is a prerequisite for study of the physiology of early development and application of assisted reproductive techniques. Oocytes were collected and then cultured in TCM-199 + 10% FBS for 4 h. The matured oocytes were activated with a 20 µsec electric pulse at 1.2 kV/mm. The activated oocytes were incubated in 2 mM of 6-dimethylaminopurine (6-DMAP) for 4 h and were then divided randomly among the treatment groups. In experiment 1, we compared the effects of three culture systems (TCM-199, CR1-aa and Tyrode's) on the in vitro development of parthenogenetically activated cat oocytes. In experiment 2, we investigated the effect of addition of Iloprost (a stable prostaglandin I2 analogue) to Tyrode's medium on in vitro development of parthenogenetically activated oocytes. As a control, we recovered in vivo produced blastocysts and determined their average cell number. In experiment 1, the cleavage frequency of the oocytes cultured in TCM199, CR1-aa and Tyrode's media were similar (74, 72 and 83%, respectively). However, the incidence of in vitro development to the blastocyst stage was significantly higher in Tyrode's medium (20.4%) than in TCM-199 (2.4%) or CR1-aa (11.1%). Likewise, the average cell number of in vitro activated blastocysts was higher in Tyrode's than in CR1-aa or TCM-199 (106.5 ± 45.2 vs. 68.3 ± 25.4 and 35.0 ± 7.7, respectively; P<0.05). In experiment 2, the percentage of parthenogenetically activated oocytes that underwent in vitro blastocyst development was significantly improved by addition of Iloprost to the culture medium (33.6 vs. 19.1%; P<0.05). The average cell number of in vivo blastocysts (909.0 ± 226.4) was significantly higher than those of in vitro blastocysts cultured in Tyrode's medium supplemented with or without Iloprost (103.2 ± 31.3 and 112.2 ± 39.3, respectively; P<0.05). This result indicated that the current culture method for cat pathenogenetically activated oocytes requires further improvement. Key words: Activation, Cat, Culture media, Prostaglandin I2 analogue (Iloprost), Oocyte (J. Reprod. Dev. 53: [1053][1054][1055][1056][1057][1058] 2007) he last decade has seen a tremendous increase in the number of studies published concerning in vitro production of cat embryos. As the number of laboratories conducting research on in vitro fertilization (IVF) and in vitro culture (IVC) of cat embryos continues to increase, so too does the variety of culture media being used. Composition of the culture medium (complex vs. simple), supplementation of hormones and gonadotropins and sources of proteins and macromolecules are among the critical components affecting the efficiency of in

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“…Johnston et al [14] demonstrated that fertilization success in vitro of matured feline follicular oocytes was high and most embryos developed equally well to the morula stage in two simple media supplemented with BSA, modified KRB (mKRB) [34] and modified Tyrode's solution (TALP) without phosphate or glucose [2], but blastocyst formation was very low in both media. Likewise, Swanson et al [32] evaluated the impact of the presence or absence of glucose and glutamine in mKRB, but the IVF-derived embryos also failed to develop into blastocysts (2-5%) under any culture conditions applied. Therefore, mKRB and TALP are inadequate as basal media for IVF and IVC in the cat.…”

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confidence: 99%

“…Inadequate culture conditions contribute to this low success rate of IVF-ET. Despite a high rate of fertilization and successful growth to morulae, IVF-derived feline embryos show a partial but highly significant in vitro developmental block at the morula-to-blastocyst stage [10,14,15,[26][27][28]32]. Moreover, conventional changes in culture conditions such as incubation temperature [15], gas atomosphere [15] and oviductal cell monolayer co-culture [27,32] have all failed to circumbent the block.…”

mentioning

confidence: 99%

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Development of in vitro Fertilized Feline Embryos in a Modified Earle's Balanced Salt Solution: Influence of Protein Supplements and Culture Dishes on Fertilization Success and Blastocyst Formation.

Kanda

Miyazaki

Kanda

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of protein supplements and culture dish type on in vitro fertilization (IVF) and embryo development in culture were examined in the domestic cat. In Experiment I, follicular oocytes were fertilized and cultured in either 1) modified Earle's balanced salt solution, designated MK-1, supplemented with one of the following: 10% human serum (HS), 10% FCS or 0.4% BSA, or 2) Medium 199 (M-199) supplemented with 10% FCS. Fertilization rates were lower (P<0.01) in MK-1 + BSA (74.4%), MK-1 + FCS (56.1%), and M-199 + FCS (51.4%) than in MK-1 + HS (94.7%). A greater (P<0.01) percentage of blastocysts was obtained in MK-1 + HS (50.0%) than in other treatment groups (range, 4.3-17.2%). In Experiment II, the effect of dish type (tissue culture dish, TCD, versus suspension culture dish, SCD) on embryo development was evaluated in MK-1 supplemented with either HS or BSA. Significantly higher proportions of IVF-derived embryos developed to blastocysts at 120 and 144 hr post-insemination, respectively, when cultured in HS/SCD (47.2 and 71.7%) than in BSA/SCD (11.4 and 27.3%) or BSA/TCD (10.4 and 25.0%). At 120 hr post-insemination, there was a lower (P<0.01) percentage of blastocysts in HS/TCD (22.2%) than in HS/SCD. In Experiment III, six embryos per cat were transferred to the uterine horns of 17 recipients at 144 hr after hCG treatment. Five of 7 recipients which received late morulae cultured in MK-1 + BSA (SCD) for 120 hr became pregnant (71.4%). Eight of 10 recipients which received early blastocysts cultured in MK-1 + HS (SCD) for 120 hr became pregnant (80.0%). We conclude that MK-1 containing HS is highly beneficial for overcoming the in vitro developmental block of IVF-derived feline embryos and increasing the success rate of IVF/ET. -KEY WORDS: developmental block, feline (domestic), human serum, in vitro culture, IVF-ET.

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Persistence of the developmental block of in vitro fertilized domestic cat embryos to temporal variations in culture conditions

Cited by 36 publications

References 37 publications

Improved felid embryo development by group culture is maintained with heterospecific companions

Improved felid embryo development by group culture is maintained with heterospecific companions

Effect of Culture Medium and Prostaglandin I2 (PGI2) Analogue on In Vitro Development of Parthenogenetically Activated Cat Oocytes

Development of in vitro Fertilized Feline Embryos in a Modified Earle's Balanced Salt Solution: Influence of Protein Supplements and Culture Dishes on Fertilization Success and Blastocyst Formation.

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