Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China

Hu, Danhe; Wu, Chao; Wang, Ruichen; Yao, Xiaohui; Nie, Kai; Lv, Qing; Fu, Shihong; Yin, Qikai; Su, Wenzhe; Li, Fan; Xu, Songtao; He, Ying; Liang, Guodong; Li, Xiangdong; Wang, Huanyu

doi:10.3390/pathogens12030490

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…NS4A and NS1 must interact to effectively synthesize viral RNA, implying that NS4A serves as a docking site for the replicase complex [83,84]. NS4A also indirectly binds double-stranded RNA by associating with host cell polyubiquitin-binding proteins, stabilizing the intermediate dsRNA genome [85].…”

Section: Non-structural Proteinsmentioning

confidence: 99%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

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Duck Tembusu Virus (DTMUV) is a pathogen of the Flaviviridae family that causes infections in poultry, leading to significant economic losses in the duck farming industry in recent years. Ducks infected with this virus exhibit clinical symptoms such as decreased egg production and neurological disorders, along with serious consequences such as ovarian hemorrhage, organ enlargement, and necrosis. Variations in morbidity and mortality rates exist across different age groups of ducks. It is worth noting that DTMUV is not limited to ducks alone; it can also spread to other poultry such as chickens and geese, and antibodies related to DTMUV have even been found in duck farm workers, suggesting a potential risk of zoonotic transmission. This article provides a detailed overview of DTMUV research, delving into its genomic characteristics, vaccines, and the interplay with host immune responses. These in-depth research findings contribute to a more comprehensive understanding of the virus’s transmission mechanism and pathogenic process, offering crucial scientific support for epidemic prevention and control.

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Section: Non-structural Proteinsmentioning

confidence: 99%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

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Isolation and characterization of a novel goose ovaritis-associated cluster 3 Tembusu virus

et al. 2023

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Detection and Genetic Analysis of Songling Virus in Haemaphysalis concinna near the China-North Korea Border

Li,

Wang

et al. 2024

Zoonoses

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Background: Songling virus (SGLV) is a spherical, enveloped, fragmented, negative-stranded RNA virus belonging to the genus Orthonairovirus in the Nairoviridae family. SGLV is transmitted by ticks and can cause disease in humans. This study identified and characterized SGLV in Haemaphysalis concinna ticks collected in 2023 in the Yanbian Korean Autonomous Prefecture (China) near the China-North Korea border. Methods: A real-time quantitative polymerase chain reaction (RT-qPCR) was used to screen for SGLV nucleic acid in ticks. Baby hamster kidney (BHK-21) and African green monkey kidney (Vero) cells were used to isolate strains of SGLV from nucleic acid-positive samples through three successive passages. Next-generation sequencing and phylogenetics methods were used to characterize the SGLVs. Results: Of the 1659 ticks collected from 6 towns in the Yanbian Korean Autonomous Prefecture near the China-North Korea border, positive SGLV nucleic acid results were identified in 19 H. concinna tick pools from Helong and Longjing towns. This discovery led to the extraction of 17 SGLV genome sequences. Homology analysis that compare the newly discovered L, M, and S segments of SGLV strain HLJ1202 revealed nucleotide similarities ranging from 95.5%–97.1%, 91.9%–98.9%, and 98.3%–99.2%, respectively, and amino acid similarities ranging from 95.7%–97.4%, 97.1%–98.8%, and 98.2%–98.9%, respectively. Six distinct clades, characterized by specific geographic locations and host organisms, were identified on the Maximum Likelihood tree of the L segment. The YB129 and YB150 isolates demonstrated SGLV nucleic acid replication across three successive passages in Vero cells, as evidenced by the decrease in RT-qPCR Ct values. Conclusion: This study marks the initial identification of SGLV in H. concinna within the Yanbian Korean Autonomous Prefecture.

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Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China

Cited by 3 publications

References 46 publications

Advancements in Research on Duck Tembusu Virus Infections

Advancements in Research on Duck Tembusu Virus Infections

Isolation and characterization of a novel goose ovaritis-associated cluster 3 Tembusu virus

Detection and Genetic Analysis of Songling Virus in Haemaphysalis concinna near the China-North Korea Border

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