Persistence of staphylococcal species and genotypes in the bovine udder

Mørk, Torill; Jørgensen, Hannah Joan; Sunde, Marianne; Kvitle, Bjørg; Sviland, Ståle; Waage, S.; Tollersrud, Tore

doi:10.1016/j.vetmic.2012.03.034

Cited by 38 publications

(63 citation statements)

References 34 publications

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“…In 62.3% (665/1060) of the samples, at least one bacterial species was isolated, and among them the genus Staphylococci was the most frequent (426/665, 64.1%). The colonization of the udder with coagulase-negative staphylococci is frequently reported in dairy herds, and it may cause mild subclinical mastitis (Mendonça et al 2012, Mork et al 2012). In our study, the cows were not examined for alterations in the mammary gland or the milk somatic cell counts were assessed; therefore, it is not possible to draw any conclusion about the implications of the coagulase-negative staphylococci isolation for the udder's health of the sampled animals.…”

Section: Discussionmentioning

confidence: 99%

“…Among the 17 positive herds, in 14 (82.3%) S. aureus was isolated in two or more sampling events indicating that S. aureus strains were circulating among the animals over time. Staphylococcus aureus is a frequent cause of clinical and subclinical mastitis, and chronically infected cows in a herd are an important reservoir of this pathogen (Ericsson et al 2009, Mork et al 2012. Considering the highly contagious nature of the infection, the presence of infected cows may constitute a serious risk of the perpetuation and spread of S. aureus to susceptible animals and the contamination of milk.…”

Section: Discussionmentioning

confidence: 99%

“…Later on, PFGE started to be considered the most discriminatory tool to resolve clonal relationship and proved to be superior to other tested techniques (Tenover et al 1994, Olive & Bean 1999. Using PFGE, it was demonstrated that S. aureus strains are more likely to be unique to a herd than to be found in multiple herds, and once introduced in a herd these strains may become persistent (Joo et al 2001, Mork et al 2012). In our study, the 122 S. aureus isolates digested by SmaI could be grouped into 30 PFGE clusters, among which three (P5, P8 and P9) encompassed strains originated from four to five different herds.…”

Section: Discussionmentioning

confidence: 99%

“…It was demonstrated that S. aureus intramammary infection can be widespread among cows within the same herd, and genotyping has shown that common clonal groups can be persistent in a herd over time (Mork et al 2012, Piccinini et al 2012. Among the genotyping techniques, pulsed-field gel electrophoresis has been frequently used to determine whether S.aureus isolates are close related and associated with persistent intramammary infection (Middleton et al 2002, Haveri et al 2008, Mork et al 2012.…”

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confidence: 99%

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Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

et al. 2016

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In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

et al. 2016

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“…warneri because it was rarely reported in former studies (Sampimon et al, 2009;Thorberg et al, 2009;Piessens et al, 2011). However, Mørk et al (2012) estimated a restricted survival period of Staph. warneri within the bovine udder.…”

Section: Variance Componentsmentioning

confidence: 99%

Quarter- and cow-level risk factors for intramammary infection with coagulase-negative staphylococci species in Swiss dairy cows

Dolder

Borne

Traversari

et al. 2017

Journal of Dairy Science

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Bacteriological status, evaluation of udder symmetry, udder hygiene, and teat end scores of 92 dairy cows were assessed on 3 Swiss dairy farms in a longitudinal 1-yr study to determine risk factors for intramammary infection (IMI) with coagulase-negative staphylococci (CNS) species. Farm visits were performed monthly including sterile quarter milk sampling and udder evaluation of all lactating cows. Milk samples were evaluated for the presence of staphylococci using selective agar plates. Species identification was performed using MALDI-TOF mass spectrometry. Intramammary infection was defined as milk samples having ≥100 cfu per mL of milk according to culture results. Overall, 3,151 quarter samples were included in the statistical analysis. Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus xylosus, and a Staphylococcus warneri-like species were the 4 most prevalent CNS species found. Hierarchical multivariable logistic regression models were built to evaluate risk factors for species-specific CNS IMI. Risk factors for Staph. chromogenes IMI were presence in herd B, the period from June 2014 to August 2014 and December 2014 to February 2015, and presence of udder edema. For Staph. haemolyticus, the relevant risk factor included coinfection with Staph. xylosus coinfection with other than the above-mentioned CNS species ("others") and the period from June 2014 to November 2014. Coinfection with Staph. haemolyticus and "others," the periods from June 2014 to August 2014 and December 2014 to February 2015, early phase of lactation (1-60 d in milk), and belonging to herd B were significantly associated with Staph. xylosus IMI. Mid and late lactation, coinfection with Staph. xylosus, and the period September 2014 to May 2015 were identified as significant risk factors for Staph. warneri-like IMI. For Staph. chromogenes, 60.6 and 26% of the variance was observed at the quarter and cow level, respectively, whereas for the other investigated species the highest variance was observed at the sample level. The predominant species within herds differed and was most pronounced for the Staph. warneri-like species.

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Transgenic technology: the strategy for the control and prevention of bovine staphylococcal mastitis?

Cardoso

Barbosa

Liberal

et al. 2019

Biotechnology Research and Innovation

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Persistence of staphylococcal species and genotypes in the bovine udder

Cited by 38 publications

References 34 publications

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

Quarter- and cow-level risk factors for intramammary infection with coagulase-negative staphylococci species in Swiss dairy cows

Transgenic technology: the strategy for the control and prevention of bovine staphylococcal mastitis?

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