Persistence of SARS-CoV-2–Specific IgG in Children 6 Months After Infection, Australia

Comparison of Seroconversion in Children and Adults With Mild COVID-19

“…We used a modified, 2-step enzyme-linked immunosorbent assay (ELISA) based on the previously described Mount Sinai Laboratory method to measure SARS-CoV-2-specific IgG responses. 26,27 Briefly, 96-well high-binding plates were coated with receptor-binding domain or S1 (Sino Biological) antigen diluted in PBS at 2 μg/mL. Serum samples were first screened with receptor-binding domain antigen; potential seropositive samples were then confirmed with S1 antigen.…”

“…Nevertheless, for the serological responses, the 3 assays used in this study are established assays for measuring SARS-CoV-2 serology and IgG, which is known to strongly correlate with neutralizing antibodies. 26,60,61…”

Hide and seek in a pandemic: review of SARS‐CoV‐2 infection and sequelae in children

“…The Children that have minimal or no symptoms of COVID-19 maintain seropositivity for at least 6 months, with no statistically significant difference compared to SARS-CoV-2 positive adults (Toh et al, 2021).…”

The COVID-19 pandemic in children and young people during 2020-2021: Learning about clinical presentation, patterns of spread, viral load, diagnosis and treatment

“…An interesting question was the duration of humoral immune response in children. Some authors detected specific IgGs 6 months after infection in children who were either asymptomatic or had mild symptoms [ 34 ]. However, other studies suggested waning of antibody responses in children 4-6 after SARS-CoV-2 infection, while parents had somewhat higher levels, but still waning [ 35 , 36 ].…”