Persistence of plasmid RP4 in Pseudomonas putida and loss of its expression of antibiotic resistance in a groundwater microcosm

Bengtsson, Göran; Fossum, Anna; Lindqvist, Roland

doi:10.1016/j.soilbio.2004.02.015

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…We subsequently showed that the plasmid pB10 and the Pseudomonas putida host adapted during 1000 generations of growth in serial batch cultures to decrease plasmid cost and instability, thereby expanding or shifting the plasmid's range of favorable hosts. The instability of IncP-1 plasmids in a strain of Pseudomonas putida is in contrast to previous studies, where IncP-1 plasmids were stably maintained in Pseudomonas putida strains KT2440 and ATCC 12633 T for at least 50 or 68 generations in serial batch cultures (Schmidhauser & Helinski, 1985;Siddique & Figurski, 2002), in Pseudomonas putida mt-2(pWW0) for 56 days in contaminated groundwater (Jain et al, 1987), in Pseudomonas putida KT2442 for up to 26 days in aquifer (Bengtsson et al, 2004), and in Pseudomonas putida UWC1 for 20 days in soil (Götz & Smalla, 1997). A parallel study in our laboratory showed that plasmid pB10 was stable in strain Pseudomonas putida UWC1 for at least 210 generations (L. De Gelder et al, unpublished data).…”

Section: Discussioncontrasting

confidence: 77%

Frequent conjugative transfer accelerates adaptation of a broad-host-range plasmid to an unfavorable Pseudomonas putida host

Heuer

Fox

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2007

FEMS Microbiology Ecology

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IncP-1 plasmids are known to be promiscuous, but it is not understood if they are equally well adapted to various species within their host range. Moreover, little is known about their fate in bacterial communities. We determined if the IncP-1beta plasmid pB10 was unstable in some Proteobacteria, and whether plasmid stability was enhanced after long-term carriage in a single host and when regularly switched between isogenic hosts. Plasmid pB10 was found to be very unstable in Pseudomonas putida H2, and conferred a high cost (c. 20% decrease in fitness relative to the plasmid-free host). H2(pB10) was then evolved under conditions that selected for plasmid maintenance, with or without regular plasmid transfer (host-switching). When tested in the ancestral host, the evolved plasmids were more stable and their cost was significantly reduced (9% and 16% for plasmids from host-switched and nonswitched lineages, respectively). Our findings suggest that IncP-1 plasmids can rapidly adapt to an unfavorable host by improving their overall stability, and that regular conjugative transfer accelerates this process.

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Section: Discussioncontrasting

confidence: 77%

Frequent conjugative transfer accelerates adaptation of a broad-host-range plasmid to an unfavorable Pseudomonas putida host

Heuer

Fox

Top

2007

FEMS Microbiology Ecology

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Persistence Mechanisms of Conjugative Plasmids

Bahl

Hansen

Sørensen

2009

Methods in Molecular Biology

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Are plasmids selfish parasitic DNA molecules or an integrated part of the bacterial genome? This chapter reviews the current understanding of the persistence mechanisms of conjugative plasmids harbored by bacterial cells and populations. The diversity and intricacy of mechanisms affecting the successful propagation and long-term continued existence of these extra-chromosomal elements is extensive. Apart from the accessory genetic elements that may provide plasmid-harboring cells a selective advantage, special focus is placed on the mechanisms conjugative plasmids employ to ensure their stable maintenance in the host cell. These importantly include the ability to self-mobilize in a process termed conjugative transfer, which may occur across species barriers. Other plasmid stabilizing mechanisms include the multimer resolution system, active partitioning, and post-segregational-killing of plasmid-free cells. Finally, various molecular adaptations of plasmids to better match the genetic background of their bacterial host cell will be described.

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Changes in Antibiotic Resistance Profile of Soil Bacterial Community in Association with Land Degradation

Doi¹,

Kaeoniam²,

Placksanoi³

et al.

Soil Mineral Microbe-Organic Interactions

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Persistence of plasmid RP4 in Pseudomonas putida and loss of its expression of antibiotic resistance in a groundwater microcosm

Cited by 4 publications

References 42 publications

Frequent conjugative transfer accelerates adaptation of a broad-host-range plasmid to an unfavorable Pseudomonas putida host

Frequent conjugative transfer accelerates adaptation of a broad-host-range plasmid to an unfavorable Pseudomonas putida host

Persistence Mechanisms of Conjugative Plasmids

Changes in Antibiotic Resistance Profile of Soil Bacterial Community in Association with Land Degradation

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