Persistence of O⁶-guanine DNA adducts in styrene-exposed lamination workers determined by ³²P-postlabelling

Abstract

A modified 32P-postlabelling method was used for the detection of styrene-specific DNA adducts in lamination workers. The persistence of O6-styrene DNA adducts was studied in DNA from lymphocytes and granulocytes of an exposed and a control group. We compared O6-adduct levels obtained from a sampling prior to vacation, after 2 weeks of vacation and after an additional 1 month of work. In granulocytes, there was no significant difference in adduct levels between the control and the exposed groups in any individual samplings. In lymphocytes of laminators the detected adduct levels were significantly higher (5.4 adducts/10(8) nucleotides) than those in the controls (1.0 adduct/10(8) nucleotides). The 2 week interruption of exposure did not influence the total O6-adduct level (4.9 adducts/10(8) nucleotides in the first sampling versus 5.1 adducts/10(8) nucleotides in the second), indicating very slow removal of the specific O6-styrene adducts from DNA.

New aspects in the biomonitoring of occupational exposure to styrene

“…Various biomarkers of styrene genotoxicity, relationships to the exposure and time of employment By evaluating all available data from our previous studies on various markers of styrene genotoxicity (Vodicka et al 1993(Vodicka et al , 1994(Vodicka et al , 1995Somorovska´et al 1999) we obtained interesting results. Only O 6 -styrene guanine DNA adducts and CA correlated with workplace styrene concentration as well as with the duration of employment.…”

“…Various biomarkers of styrene genotoxicity, relationships to exposure, and time of employment Analyses of O 6 -styrene guanine DNA adducts, N-terminal valine adducts in haemoglobin, SBs in DNA, CA and the mutant frequency in the HPRT gene were reported by us as separate studies earlier (Vodicka et al 1993(Vodicka et al , 1994(Vodicka et al , 1995Somorovska´et al 1999). Statistical calculations were based on following individual values from all our previous population studies: haemoglobin adducts, n=16, O 6 -styrene guanine DNA adducts, n=38, SBs in DNA, n=41, CA, n=56 and HPRT MF, n=35.…”

“…The levels of O 6 -and N 2 -guanine DNA adducts of styrene in white blood cells of lamination workers have been previously detected by the 32 P-post-labelling method (Horvath et al 1994;Vodicka et al 1994). Most recently, bN1-SO-adenine adducts were detected in workers exposed to styrene, at a level of 0.08 adducts/10 8 nucleotides ).…”

Syntheses of O6‐Alkyl‐ and Arylguanine Derivatives: Nucleobase Adducts Derived from Styrene 7,8‐ and 3,4‐Oxides

“…[3,4] The residues of modified DNA − DNA adducts − can be determined in various tissues as nucleotide adducts or in urine as modified nucleobases. [5,6] In our previous work we described syntheses of 7-alkylguanines derived from styrene 7,8-oxide (1), a carcinogenic metabolite of styrene. [7] To extend the range of nucleobase adducts that can be used as markers of exposure to styrene, we hereby present synthetic routes leading to O 6 -alkylguanines derived from styrene 7,8-oxide (1) and O 6 -arylguanines derived from arene oxide metabolites of styrene, such as styrene 3,4-oxide (2), 4-(2-hydroxyethyl)benzene 1,2-oxide (3) and 4-carboxymethylbenzene 1,2-oxide (4).…”

The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis

“…DNA adducts induced by styrene and SO have been consistently identified in human cells, experimental animals, and occupationally exposed individuals [3,[57][58][59]. In vitro studies with human lymphocytes have demonstrated SO ability to form DNA adducts and to cause single-strand breaks and cytogenetic alterations in a dose-related manner [60,61].…”