Persistence of E2A/PBX1 transcripts in t(1;19) childhood acute lymphoblastic leukemia: Correlation with chemotherapy intensity and clinical outcome

Lucchesi, Carlo; Gottardi, Enrico; Gaïdano, Gianluca; Vivenza, C; Parziale, Adele; Perfetto, Fatima; Fornaci, Marinella; Barisone, Elena; Madon, E; Basso, Giuseppe; Saglio, Giuseppe

doi:10.1016/0145-2126(95)00115-8

Cited by 16 publications

(6 citation statements)

References 4 publications

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“…The incidence of the E2A/PBX1 fusion was 8.6%, which coincides with other published study results [22]. Some clinical features, such as age and immunophenotype (T-ALL in one child), found in our patients, differ from literature reports.…”

Section: Discussionsupporting

confidence: 69%

Clinical features of the most common fusion genes in childhood acute lymphoblastic leukemia

Lazić

Tos̆ić²,

Dokmanović

et al. 2009

Med Oncol

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Contemporary protocols ensure high-remission rate and long-term free survival in children with acute lymphoblastic leukemia (ALL), but small percentage of patients is still incurable. Molecular genetic methods helped to establish submicroscopic classification as well as minimal residual disease follow-up, considered to be responsible for relapse. Our study enrolled 70 pediatric patients with de novo ALL, analyzed using reverse transcriptase-polymerase chain reaction for the presence of four major risk-stratifying translocations (BCR/ABL, MLL/AF4, TEL/AML1, and E2A/PBX1). Bone marrow samples were collected at diagnosis, at the end of induction phase, and after intensive chemotherapy with the aim to establish the correlation between chromosomal aberration, clinical features, and treatment response. Presenting the results of this study, we offer another evidence of variable incidence and clinical characteristics of ALL subtypes.

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Section: Discussionsupporting

confidence: 69%

Clinical features of the most common fusion genes in childhood acute lymphoblastic leukemia

Lazić

Tos̆ić²,

Dokmanović

et al. 2009

Med Oncol

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“…integrity a control amplification was performed, as described elsewhere. 13 Positive and negative controls were included 4997-5001. throughout. this rearrangement in DS individuals with ALL (P Ͼ 0.05; Fish-…”

Section: Patients and Methods Cases Tested This Data May Be Suggestimentioning

confidence: 99%

The common TEL/AML1 rearrangement does not represent a frequent event in acute lymphoblastic leukaemia occurring in children with Down syndrome

et al. 1997

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“…[65][66][67] Several studies using RT-PCR amplification of E2A-PBX1 FG transcripts to assess MRD have been reported. [68][69][70][71][72] All of them were performed with a qualitative assay showing a detection threshold of up to 10 À4/À5 . In none of these reports does the presence or absence of E2A-PBX1 transcripts during follow-up predict treatment outcome.…”

Section: Introductionmentioning

confidence: 99%

Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia – A Europe Against Cancer Program

et al. 2003

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Detection of minimal residual disease (MRD) has proven to provide independent prognostic information for treatment stratification in several types of leukemias such as childhood acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML) and acute promyelocytc leukemia. This report focuses on the accurate quantitative measurement of fusion gene (FG) transcripts as can be applied in 35-45% of ALL and acute myeloid leukemia, and in more than 90% of CML. A total of 26 European university laboratories from 10 countries have collaborated to establish a standardized protocol for TaqManbased real-time quantitative PCR (RQ-PCR) analysis of the main leukemia-associated FGs within the Europe Against Cancer (EAC) program. Four phases were scheduled: (1) training, (2) optimization, (3) sensitivity testing and (4) patient sample testing. During our program, three quality control rounds on a large series of coded RNA samples were performed including a balanced randomized assay, which enabled final validation of the EAC primer and probe sets. The expression level of the nine major FG transcripts in a large series of stored diagnostic leukemia samples (n ¼ 278) was evaluated. After normalization, no statistically significant difference in expression level was observed between bone marrow and peripheral blood on paired samples at diagnosis. However, RQ-PCR revealed marked differences in FG expression between transcripts in leukemic Correspondence: Professor J

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Persistence of E2A/PBX1 transcripts in t(1;19) childhood acute lymphoblastic leukemia: Correlation with chemotherapy intensity and clinical outcome

Cited by 16 publications

References 4 publications

Clinical features of the most common fusion genes in childhood acute lymphoblastic leukemia

Clinical features of the most common fusion genes in childhood acute lymphoblastic leukemia

The common TEL/AML1 rearrangement does not represent a frequent event in acute lymphoblastic leukaemia occurring in children with Down syndrome

Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia – A Europe Against Cancer Program

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