Persistence of donor cells in neonates after fetal and exchange transfusion

Hutchinson, Donald L.; Turner, John T.; Schlesinger, Edward R.

doi:10.1016/0002-9378(71)90876-3

Cited by 68 publications

(29 citation statements)

References 5 publications

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“…However, to our knowledge, no previous study has specifically examined longterm persistence of maternal cells in immunologically competent offspring. Supporting the possibility, in an earlier study that used cytogenetic analysis, male infants who received in utero transfusion from their mothers were found to harbor up to 4% of lymphocytes, with 2 X chromosomes at 4-5 years of age (27). In a murine model, maternal cells were found routinely in the bone marrow of immunocompetent fetuses (28).…”

Section: Discussionmentioning

confidence: 88%

Microchimerism of maternal origin persists into adult life

Maloney¹,

Smith²,

Fürst³

et al. 1999

J. Clin. Invest.

411

302

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Section: Discussionmentioning

confidence: 88%

Microchimerism of maternal origin persists into adult life

Maloney¹,

Smith²,

Fürst³

et al. 1999

J. Clin. Invest.

411

302

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“…In another study by Schechter et al, 4 the authors found circulating donor lymphocytes up to a week in adult patients who had transfusion of moderate to large amounts of blood. Hutchinson et al 5 in a study compared the presence of donor cells in exchange transfusion with donor blood from blood bank versus fetal transfusion of maternal cells in newborns. They found that 10 of the 48 infants receiving donor blood transfusion demonstrated donor cells ranging in frequency from 1 to 9%, 2-4 weeks posttransfusion.…”

Section: Discussionmentioning

confidence: 99%

Packed red cell transfusion does not compromise chromosome analysis in newborns

Kulharya

Salbert

Norris

et al. 2001

Genetics in Medicine

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Purpose: Critically ill neonates are frequently transfused with packed red cells. Some of these transfused neonates also need chromosome analysis. There is a long-standing tradition in pediatrics of not performing chromosome analysis after transfusion. We wished to determine whether transfusion with packed red cells affect the cytogenetic results in neonates. Method: The medical records of all neonates at the Medical College of Georgia who had had chromosome analysis between June 1995 and June 1998 were reviewed. Ten neonates had received transfusion prior to cytogenetic testing. Of these 10 infants, two had been transfused two or more times. Routine cytogenetic analysis of 20 metaphases at 550-band level had been performed on all 10 patients. Heteromorphic markers were compared in 10 randomly selected metaphases for any discrepancy. To determine whether there were theoretical reasons to delay chromosome analysis in transfused neonates, samples of irradiated, and/or filtered, and nonfiltered blood were obtained from the blood bank and analyzed for the presence of lymphocytes. Results: Prior transfusion did not affect karyotype results. A nonmosaic abnormal karyotype was found in 3 of the 10 patients.A fourth patient's karyotype was 45,X/47,XXX. This mosaicism was constitutive and consistent as demonstrated by a follow-up chromosome analysis. All other abnormal karyotypes were consistent with the dysmorphic phenotype. Randomly selected metaphases did not show any differences in the identifiable heteromorphic markers in all 10 patients. Although there was a 50% chance of patients receiving blood from a donor of opposite sex, there were no instances in which cells with a karyotype of the opposite sex were found in the patients' blood. The irradiated and filtered cultured donor blood samples did not show any metaphases. However, metaphases were seen in the cultures from nonfiltered and nonirradiated donor blood. Conclusions: Based on these results one does not need to delay karyotyping babies who have had blood transfusions. Packed red cell transfusion in newborns does not compromise the accuracy of chromosome analysis in our study even with multiple transfusions. Genetics in Medicine, 2001:3(4):314 -317.

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“…We used Y-chromosome DNA amplification for screening for microchimerism because of its ready applicability to female recipients receiving blood from one or more male donors of otherwise unknown genotype. Historically, the detection of microchimerism capitalized on such sex chromosome differences, primarily through cytogenetic identification of the Y chromosome 8,11 or through PCR amplification of Y-chromosome DNA fragments. 9,10 More recently, it has become possible to study other polymorphic genetic markers, such as HLA alleles, 27,34 or non-HLA, highly polymorphic, or otherwise informative markers such as are found on human globin genes.…”

Section: Survival Of Transfused Donor White Blood Cells 275mentioning

confidence: 99%

“…7,8 In immunologically intact persons, the life of such cells is usually short (6 or fewer days), 9,10 and no clinical consequences result. However, prolonged survival of donor cells, sometimes for years, has occurred after intrauterine transfusion, 11,12 and recent studies have demonstrated the asymptomatic persistence of minor populations of donor cells in a proportion of patients after massive transfusion for trauma. 13 In immunologically impaired recipients, however, such as children with severe combined immunodeficiency, the consequences of transfusing viable WBCs in blood components can include graft-versus-host disease (GVHD).…”

Section: Introductionmentioning

confidence: 99%

Survival of transfused donor white blood cells in HIV-infected recipients

Kruskall

Lee

Assmann

et al. 2001

Blood

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The appearance and expansion of donor white blood cells in a recipient after transfusion has many potential biologic ramifications. Although patients with HIV infection are ostensibly at high risk for microchimerism, transfusion-associated graft-versus-host disease (TA-GVHD) is rare. The purpose of this study was to search for sustained microchimerism in such patients. Blood samples were collected from 93 HIV-infected women (a subset from the Viral Activation Transfusion Study, an NHLBI multicenter randomized trial comparing leukoreduced versus unmodified red blood cell [RBC] transfusions) before and after transfusions from male donors. Donor lymphocytes were detected in posttransfusion specimens using a quantitative Y-chromosome-specific polymerase chain reaction (PCR) assay, and donor-specific human leukocyte antigen (HLA) alleles were identified with allele-specific PCR primers and probes. Five of 47 subjects randomized to receive nonleukoreduced RBCs had detectable male lymphocytes 1 to 2 weeks after transfusion, but no subject had detectable male cells more than 4 weeks after a transfusion. In 4 subjects studied, donorspecific HLA haplotypes were detected in posttransfusion specimens, consistent with one or more donors' cells. None of 46 subjects randomized to receive leukoreduced RBCs had detectable male lymphocytes in the month after transfusion. Development of sustained microchimerism after transfusion in HIV-infected patients is rare; HIV-infected patients do not appear to be at risk for TA-GVHD. (Blood. 2001;98:272-279)

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Persistence of donor cells in neonates after fetal and exchange transfusion

Cited by 68 publications

References 5 publications

Microchimerism of maternal origin persists into adult life

Microchimerism of maternal origin persists into adult life

Packed red cell transfusion does not compromise chromosome analysis in newborns

Survival of transfused donor white blood cells in HIV-infected recipients

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