Persistence of dicentrics in Chernobyl clean-up workers who suffered from low doses of radiation

Slozina, N M; Neronova, E.G.; Nikiforov, A.M.

doi:10.1016/s0969-8043(01)00059-8

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Results with longer follow-up times have been reported for clean-up workers involved in the Chernobyl accident. Slozina et al [2001] as well as others cited by them found the frequency of lymphocytes containing dicentrics and rings increased rather than decreased with time up to 12 years after exposure. Their data are in disagreement with those reports suggesting that damaged lymphocytes disappear from the peripheral blood with a half-life of 1 or 3 years, but it should not be dismissed easily especially as they come from several different laboratories.…”

mentioning

confidence: 65%

“…Similarly, the dicentric and acentric data published by Mészáros et al [2004] showed a decrease, albeit less than expected under the assumption of a lymphocyte half-life of 3 years and not significant in the case of dicentrics. On the other hand, data from the Chernobyl clean-up workers, as summarized by Slozina et al [2001], indicated an increase in unstable chromosome aberration frequency with time after exposure. As mentioned in the introduction, we think that these findings cannot easily be dismissed, even though they are not in agreement with the general thinking about the stability of the genetic damage in question.…”

Section: Discussionmentioning

confidence: 99%

“…It would therefore seem that only (or preferentially) densely ionizing radiation in particular is able to induce genomic instability. On the other hand, the persistence of dicentrics in Chernobyl clean-up workers, mentioned in the introduction [Slozina et al, 2001], speaks against such an assumption, as exposure in these cases stems mainly from sparsely ionizing radiation [Nuclear Energy Agency, 2002].…”

Section: Discussionmentioning

confidence: 99%

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Persistence of Genetic Damage in Lymphocytes from Former Uranium Miners

Zölzer

Hon²,

Skalická³

et al. 2012

Cytogenet Genome Res

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Lymphocytes from former uranium miners who finished work underground one or more decades ago were analysed with respect to possibly persisting genetic damage induced by their radiation exposure. A modified micronucleus-centromere test was used which determined the frequency of micronucleus-containing binucleate cells after cytochalasin B treatment and the percentage of centromere-free micronuclei, assessed with the help of immunofluorescence labeling of centromere protein B. Whereas the overall frequency of micronucleus-containing cells was not significantly elevated above the level found in a control group, former miners showed a greater percentage of centromere-free micronuclei, i.e. micronuclei containing only acentric fragments. Our results are in excellent agreement with those of an earlier uranium miner study and lend support to the assumption that genetic damage from alpha radiation can persist for many years after exposure, possibly due to genomic instability. The frequency of micronucleus-containing cells, but not the percentage of centromere-free micronuclei, significantly increased with time since last exposure in the mines. This can be attributed, at least in part, to the fact that miners who have finished working underground longer ago tend to be older, and there is an increase of the frequency of micronucleus-containing cells with age.

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mentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Persistence of Genetic Damage in Lymphocytes from Former Uranium Miners

Zölzer

Hon²,

Skalická³

et al. 2012

Cytogenet Genome Res

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“…In general, the dicentric chromosome assay (DCA) is considered to be the gold standard for such biodosimetry. It has been widely used to evaluate radiation doses of accidentally and occupationally exposed persons (Slozina et al 2001 ; Ramalho and Nascimento 1991 ; Suto et al 2013 ; Chung et al 1996 ), but it might be not suitable for larger- scale radiological accidents due to multiple drawbacks: it is labor-intensive, time-consuming, and requires highly skilled personnel.…”

Section: Introductionmentioning

confidence: 99%

Improving radiation dosimetry with an automated micronucleus scoring system: correction of automated scoring errors

Lee

Jin

Seong

et al. 2023

Radiat Environ Biophys

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Radiation dose estimations performed by automated counting of micronuclei (MN) have been studied for their utility for triage following large-scale radiological incidents; although speed is essential, it also is essential to estimate radiation doses as accurately as possible for long-term epidemiological follow-up. Our goal in this study was to evaluate and improve the performance of automated MN counting for biodosimetry using the cytokinesis-block micronucleus (CBMN) assay. We measured false detection rates and used them to improve the accuracy of dosimetry. The average false-positive rate for binucleated cells was 1.14%; average false-positive and -negative MN rates were 1.03% and 3.50%, respectively. Detection errors seemed to be correlated with radiation dose. Correction of errors by visual inspection of images used for automated counting, called the semi-automated and manual scoring method, increased accuracy of dose estimation. Our findings suggest that dose assessment of the automated MN scoring system can be improved by subsequent error correction, which could be useful for performing biodosimetry on large numbers of people rapidly, accurately, and efficiently.

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