Persistence of Ca2+-Sensing Receptor Expression in Functionally Active, Long-Term Human Parathyroid Cell Cultures

Roussanne, M C; Gogusev, Jean; Hory, B; Duchambon, Patricia; Souberbielle, Jean Claude; Nabarra, B; D, Pierrat; Sarfati, E.; Drüeke, Tilman B.; Bourdeau, A.

doi:10.1359/jbmr.1998.13.3.354

Cited by 40 publications

(25 citation statements)

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“…One possible explanation for a direct effect on set-points would be increased expression of calcium receptors, CaR and/or LRP2/megalin. Even though LRP2/megalin expression was down-regulated, CaR expression was maintained in human parathyroid cells cultured by Roussanne et al (1998). Our own and other authors' previous data from bovine parathyroid cells indicate that vitamin D 3 , atRA and 9cRA do not affect CaR or LRP2/megalin mRNA expression (Rogers et al 1995, Brown et al 1996, Hellman et al 1998.…”

Section: +mentioning

confidence: 66%

“…Many attempts to establish parathyroid cells in culture and parathyroid cell lines have been undertaken, but only occasional reports have indicated success (Brandi et al 1986, Roussanne et al 1998 and it has been difficult to repeat some of these data. Most attempts were performed utilizing bovine parathyroid cells, whereas our aim was to culture human parathyroid cells obtained from parathyroid adenomas.…”

Section: Discussionmentioning

confidence: 99%

“…The previous method of Roussanne et al (1998) utilized cells derived from patients with uremic hyperparathyroidism, and highphosphate serum containing medium, which also resulted in proliferating human parathyroid cells. Thus, it is possible to obtain dividing human parathyroid cell cultures, which may be used for a number of interesting studies.…”

Section: +mentioning

confidence: 99%

“…There is only one report documenting successful long-term human parathyroid cultures, and the only cell line reported to be derived from parathyroid cells is of rat origin and does not produce PTH (Sakaguchi et al 1987, Roussanne et al 1998. In this report we describe one model that enables studies of cell proliferation and calcium sensing in human parathyroid cells derived from adenomas cultured in a serum-free medium.…”

Section: Introductionmentioning

confidence: 97%

“…However, success with continuous culture of bovine cells has in our (and many other) hands been limited by the overgrowth of fibroblasts after 5-10 days, although there are reports in the literature describing successful culture involving an impressive number of doublings (Brandi et al 1986). Also, there seems to be a difference between bovine and human parathyroid cells, since CaR expression in the latter is retained in culture (Roussanne et al 1998). …”

Section: Introductionmentioning

confidence: 99%

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Differentiation of human parathyroid cells in culture

Liu¹,

Ridefelt²,

Åkerström³

et al. 2001

Journal of Endocrinology

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Continuous culture of parathyroid cells has proven difficult, regardless from which species the cells are derived. In the present study, we have used a defined serum-free low calcium containing medium to culture human parathyroid cells obtained from patients with parathyroid adenomas due to primary hyperparathyroidism. No fibroblast overgrowth occurred, and the human parathyroid chief cells proliferated until confluent. After the first passage the cells ceased to proliferate, but still retained their functional capacity up to 60 days, demonstrated by Ca 2+ -sensitive changes in the release of parathyroid hormone (PTH) and as adequate cytoplasmic calcium ([Ca 2+ ] i ) responses to changes in ambient calcium as measured by microfluorimetry. Low calcium concentrations enhanced, and vitamin D 3 and retinoic acids (RA) dose-dependently inhibited cell proliferation during the first passage, as determined by [ 3 H]thymidine incorporation, immunohistochemistry for proliferating cell nuclear antigen and cell counting. Signs of differentiation were present as the set-points, defined as the external calcium concentration at which half-maximal stimulation of [Ca 2+ ] i (set-point c ), or half-maximal inhibition of PTH release (set-point p ) occur, were higher in not proliferating compared with proliferating cells in P0. Inhibition of cell proliferation was accompanied by signs of left-shifted set-points, indicating a link between proliferation and differentiation.The results demonstrate that human parathyroid chief cells cultured in a defined serum-free medium can be kept viable for a considerable time, and that signs of differentiation occur after proliferation has ceased. The low calcium stimulated cell proliferation may also be inhibited by vitamin D and RA.

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Section: +mentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differentiation of human parathyroid cells in culture

Liu¹,

Ridefelt²,

Åkerström³

et al. 2001

Journal of Endocrinology

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An Overview of Extracellular Calcium Homeostasis and the Roles of the CaR in Parathyroid and C-cells

Mithal¹,

Brown²

2003

Endocrine Updates

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Culture of Parathyroid Cells

Björklund

Hellman

2011

Methods in Molecular Biology

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The parathyroid cells are highly differentiated with more or less their only function to secrete parathyroid hormone in response to the extracellular calcium level. Tumours from the parathyroid glands are >99% benign, and have a slow proliferation rate. Culture of parathyroid cells is known to be very difficult most likely due to the high differentiation level. This chapter reveals some details in order how to get parathyroid cells to survive in culture after dispersion of normal bovine parathyroid glands or pathological human parathyroid tumours. Detailed protocols describing cell dispersion with collagenase, short-term cultures, and establishment of long-term cultures are presented.

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Persistence of Ca2+-Sensing Receptor Expression in Functionally Active, Long-Term Human Parathyroid Cell Cultures

Abstract: ABSTRACT

Cited by 40 publications

References 39 publications

Differentiation of human parathyroid cells in culture

Differentiation of human parathyroid cells in culture

An Overview of Extracellular Calcium Homeostasis and the Roles of the CaR in Parathyroid and C-cells

Culture of Parathyroid Cells

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