Persistence of Arcobacter butzleri CCUG 30484 on plastic, stainless steel and glass surfaces

Červenka, Libor; Kristlova, Jana; Peskova, Iva; Vytřasová, Jarmila; Pejchalová, Marcela; Brožková, Iveta

doi:10.1590/s1517-83822008000300021

Cited by 13 publications

(7 citation statements)

References 11 publications

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“…The results showed that the mean roughness of a material is not a proof of cleanability and hygienic quality. In 2008 Cervenka et al used a similar method on plastic and stainless steel to study the persistence of Arcobacter butzleri on these two surfaces (25 cm²) [40]. In this study, sonication was applied at 38 kHz for 5 minutes and the results proved that Arcobacter could survive for a long time on a surface with a low surface free energy like plastic compared to a higher surface free energy like metal.…”

Section: Destructive Recovery Methodsmentioning

confidence: 76%

“…Cervenka et al described a “coupon-printing method” which was tested only in the laboratory. Stainless steel, polypropylene and glass 1.5 × 1.5 cm chips were put on a selective agar medium for 5 minutes then aseptically recovered in order to incubate the plates and observe the viable cells [40]. The results were used to compare the persistence of bacteria on three surfaces and it was concluded that Arcobacter butzleri was more persistent on plastic.…”

Section: Non-destructive Recovery Methodsmentioning

confidence: 99%

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Methods for Recovering Microorganisms from Solid Surfaces Used in the Food Industry: A Review of the Literature

Ismaïl

Aviat

Michel

et al. 2013

IJERPH

101

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Various types of surfaces are used today in the food industry, such as plastic, stainless steel, glass, and wood. These surfaces are subject to contamination by microorganisms responsible for the cross-contamination of food by contact with working surfaces. The HACCP-based processes are now widely used for the control of microbial hazards to prevent food safety issues. This preventive approach has resulted in the use of microbiological analyses of surfaces as one of the tools to control the hygiene of products. A method of recovering microorganisms from different solid surfaces is necessary as a means of health prevention. No regulation exists for surface microbial contamination, but food companies tend to establish technical specifications to add value to their products and limit contamination risks. The aim of this review is to present the most frequently used methods: swabbing, friction or scrubbing, printing, rinsing or immersion, sonication and scraping or grinding and describe their advantages and drawbacks. The choice of the recovery method has to be suitable for the type and size of the surface tested for microbiological analysis. Today, quick and cheap methods have to be standardized and especially easy to perform in the field.

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Section: Destructive Recovery Methodsmentioning

confidence: 76%

Section: Non-destructive Recovery Methodsmentioning

confidence: 99%

Methods for Recovering Microorganisms from Solid Surfaces Used in the Food Industry: A Review of the Literature

Ismaïl

Aviat

Michel

et al. 2013

IJERPH

101

View full text Add to dashboard Cite

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“…Both characteristics have been described in some zoonotic food-borne pathogens like Listeria monocytogenes [10,21] and Yersinia enterocolitica [10,23]. In the case of Arcobacter butzleri, a few studies on attachment to inert surfaces [1,4,18] and in vitro adhesion to epithelial cells [3,11,22,28,29] are available.…”

Section: Discussionmentioning

confidence: 99%

“…First, A. butzleri adhere to HEp-2 cells and then, due to their capacity to form biofilms, interbacterial adherence can be expressed. Previous studies have demonstrated the capacity of A. butzleri to form biofilms while attaching to stainless steel, copper and glass [1,4,18].…”

Section: Discussionmentioning

confidence: 99%

Arcobacter butzleri strains isolated from different sources display adhesive capacity to epithelial cells in vitro

Fernández

Flores

Inzunza

2018

Acta Scientiae. Vet.

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Background:The genus Arcobacter, belonging to the family Campylobacteraceae, includes polar flagellated, curved or spiral rod shaped bacteria firstly described as Vibrio/Spirillum organisms and later as aerotolerant Campylobacterlike microorganisms. Currently, this genus comprises nine validated species and a candidatus. Among Arcobacter species, Arcobacter butzleri is recognized as a zoonotic agent and the most common species of the genus isolated from environmental water, food and clinical samples.Recently, this species was considered a serious hazard to human health. However, their pathological properties, potential virulence factors as well as their clinical significance remain uncertain or not completely defined. The aim of this study was to establish the ability of 78 A. butzleri strains isolated from different sources to adhere to HEp-2 cells in vitro.Materials, Methods & Results: All the strains were isolated using first an enrichment medium incubated aerobically at 26 ºc for 48 h. after that, 100 µl of the broth were streaked onto Arcobacter selective agar plates and incubated at 26 °c for 72 h. all the strains were identified phenotypically using standard assays. Definitive identification was achieved using a multiplex polymerase chain reaction. Adhesive capacity was determined infecting hep-2 cells with the strains under study following scaletsky's et al. protocol, with slight modifications. An adherent Escherichia coli isolated was included as a positive control, whereas uninoculated cell lines were used as negative controls. Adhesion results were expressed as the percentage of hep-2 cells showing adhering bacteria and the number of bacteria (± sd) adhered to cells was determined. Strains were considered as adherent, if at least 20% of hep-2 cells showed one ore more adhered bacteria. Adherence was also demonstrated by scanning electronic microscopy. All the strains showed adhesive capacity to HEp-2 cells in vitro. The lowest adhesion percentage (27%) was observed in a strain isolated from dog feces. In at least one strain per source it was possible to observe 100% adhesion. The lowest number of adhered bacteria (1.03 ± 1.53) was observed in a strain isolated from river water and the highest number (76.6 ± 5.59), in a strain isolated from duck feces.Discussion: Regardless of their isolation source, the 78 Arcobacter butzleri strains tested in the present study were able to adhere to HEp-2 cells in vitro. This property was demonstrated in different proportions by other authors in strains isolated from environmental, meat and human fecal samples. Electronic scanning microscopy shows curved bacilli adhering to HEp-2 cells forming a microcolony-like arrangement. This may occur in a stepwise mechanism. First, A. butzleri adhere to HEp-2 cells and then, due to their capacity to form biofilms, interbacterial adherence can be expressed. The capacity of A. butzleri to form biofilms attaching to stainless steel, copper and glass has been demonstrated in previous studies. Further studies are necessary to elucida...

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“…Extended survival times have been reported for A. butzleri cells on polypropylene at high humidity, particularly in soiled conditions (Cervenka et al, 2008). Extended survival times have been reported for A. butzleri cells on polypropylene at high humidity, particularly in soiled conditions (Cervenka et al, 2008).…”

Section: Controlmentioning

confidence: 97%

Arcobacter Species

Score

Phillips

2015

Food Safety

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Persistence of Arcobacter butzleri CCUG 30484 on plastic, stainless steel and glass surfaces

Cited by 13 publications

References 11 publications

Methods for Recovering Microorganisms from Solid Surfaces Used in the Food Industry: A Review of the Literature

Methods for Recovering Microorganisms from Solid Surfaces Used in the Food Industry: A Review of the Literature

Arcobacter butzleri strains isolated from different sources display adhesive capacity to epithelial cells in vitro

Arcobacter Species

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