Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest

Rahube, Teddie O.; Marti, Romain; Scott, Andrew; Tien, Yuan Ching; Murray, Roger; Sabourin, Lyne; Duenk, Peter; Lapen, David R.; Topp, Edward

doi:10.1139/cjm-2016-0034

Cited by 43 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human and animal waste is often recycled and used to fertilise agricultural land; this can contain high concentrations and varieties of ARGs which can be passed on to people (Meek, Vyas and Piddock 2015 ; Rahube et al. 2016 ). One study performed in Canada found in the first year vegetables grown above, on and below the surface of soil treated with sewage contained significantly more ARGs than non-treated soil (Rahube et al.…”

Section: Introductionmentioning

confidence: 99%

Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing

Buckner

Ciusa

Piddock

2018

FEMS Microbiology Reviews

146

103

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a global problem hindering treatment of bacterial infections, rendering many aspects of modern medicine less effective. AMR genes (ARGs) are frequently located on plasmids, which are self-replicating elements of DNA. They are often transmissible between bacteria, and some have spread globally. Novel strategies to combat AMR are needed, and plasmid curing and anti-plasmid approaches could reduce ARG prevalence, and sensitise bacteria to antibiotics. We discuss the use of curing agents as laboratory tools including chemicals (e.g. detergents and intercalating agents), drugs used in medicine including ascorbic acid, psychotropic drugs (e.g. chlorpromazine), antibiotics (e.g. aminocoumarins, quinolones and rifampicin) and plant-derived compounds. Novel strategies are examined; these include conjugation inhibitors (e.g. TraE inhibitors, linoleic, oleic, 2-hexadecynoic and tanzawaic acids), systems designed around plasmid incompatibility, phages and CRISPR/Cas-based approaches. Currently, there is a general lack of in vivo curing options. This review highlights this important shortfall, which if filled could provide a promising mechanism to reduce ARG prevalence in humans and animals. Plasmid curing mechanisms which are not suitable for in vivo use could still prove important for reducing the global burden of AMR, as high levels of ARGs exist in the environment.

show abstract

Section: Introductionmentioning

confidence: 99%

Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing

Buckner

Ciusa

Piddock

2018

FEMS Microbiology Reviews

146

103

View full text Add to dashboard Cite

show abstract

“…These bacteria have proven particularly difficult to treat for a few key reasons; one is that the genes coding for ESBLs and carbapenemases are most often carried on plasmids, most of which are conjugative. These AMR plasmids and ARGs are found within, and shared between, bacteria colocalizing the same niche, such as intestinal tracts (9)(10)(11); environments, including wastewater and river sediments (12); agricultural soil (13)(14)(15); and hospital surfaces (16,17). Furthermore, ARGs frequently located on plasmids have been acquired by people traveling to areas of the world with high levels of AMR (18)(19)(20).…”

mentioning

confidence: 99%

HIV Drugs Inhibit Transfer of Plasmids Carrying Extended-Spectrum β-Lactamase and Carbapenemase Genes

Buckner

Ciusa

Meek

et al. 2020

mBio

View full text Add to dashboard Cite

Antimicrobial-resistant (AMR) infections pose a serious risk to human and animal health. A major factor contributing to this global crisis is the sharing of resistance genes between different bacteria via plasmids. The WHO lists Enterobacteriaceae, such as Escherichia coli and Klebsiella pneumoniae, producing extended-spectrum β-lactamases (ESBL) and carbapenemases as “critical” priorities for new drug development. These resistance genes are most often shared via plasmid transfer. However, finding methods to prevent resistance gene sharing has been hampered by the lack of screening systems for medium-/high-throughput approaches. Here, we have used an ESBL-producing plasmid, pCT, and a carbapenemase-producing plasmid, pKpQIL, in two different Gram-negative bacteria, E. coli and K. pneumoniae. Using these critical resistance-pathogen combinations, we developed an assay using fluorescent proteins, flow cytometry, and confocal microscopy to assess plasmid transmission inhibition within bacterial populations in a medium-throughput manner. Three compounds with some reports of antiplasmid properties were tested; chlorpromazine reduced transmission of both plasmids and linoleic acid reduced transmission of pCT. We screened the Prestwick library of over 1,200 FDA-approved drugs/compounds. From this, we found two nucleoside analogue drugs used to treat HIV, abacavir and azidothymidine (AZT), which reduced plasmid transmission (AZT, e.g., at 0.25 μg/ml reduced pCT transmission in E. coli by 83.3% and pKpQIL transmission in K. pneumoniae by 80.8% compared to untreated controls). Plasmid transmission was reduced by concentrations of the drugs which are below peak serum concentrations and are achievable in the gastrointestinal tract. These drugs could be used to decolonize humans, animals, or the environment from AMR plasmids. IMPORTANCE More and more bacterial infections are becoming resistant to antibiotics. This has made treatment of many infections very difficult. One of the reasons this is such a large problem is that bacteria are able to share their genetic material with other bacteria, and these shared genes often include resistance to a variety of antibiotics, including some of our drugs of last resort. We are addressing this problem by using a fluorescence-based system to search for drugs that will stop bacteria from sharing resistance genes. We uncovered a new role for two drugs used to treat HIV and show that they are able to prevent the sharing of two different types of resistance genes in two unique bacterial strains. This work lays the foundation for future work to reduce the prevalence of resistant infections.

show abstract

“…Antimicrobial residues are components of animal manure, human sewage, and aquaculture alongside fecal bacteria and resistance genes (Ruuskanen et al, 2016;Wang et al, 2018). Waste treatment and manure composting decrease levels of certain but not all antimicrobials and microorganisms that are brought to the soil after land use of person and animal biosolids (Rahube et al, 2016). Antibiotic residues can contribute to the development of antibiotic resistance in bacteria through selective pressure (Laxminarayan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Effects of Bee Venom From (Apis Mellifera) on Multidrug-Resistant Bacteria (Mdrb)

Fadl

2018

Al-Azhar Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

The prevalence of (MDRB) is increasing worldwide; therefore, this study aimed to identify the most common MDRB in clinical specimens and meet the urgent need to develop new antibacterial drugs to control their intractable infection. Additionally, due to the confrontation of the infection associated with bacterial biofilms, which are difficult to treat, and cause problems to public health, which require real solutions. Bee Venom produced by the glands of (Apis mellifera) is a complex mixture of active peptides, enzymes, and amines. So, it is considered a fertile environment for research to achieve the goal of this study. The results of the specimen's examination showed that, from a total of 500 clinical specimens, there are 224 specimens exhibited no growth, while 276 were positive. From 276 positive cultures, 317 isolates were obtained. Out of the 317 bacterial isolates, 169 (53.3%) were Gram-negative bacteria (GNB), and 148 (46.7%) were Grampositive (GP). It was of this number 124 (39.1%) were multidrug-resistant (MDR) isolates of which 89 (71.77%) were Gram-negative type, including Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and Pseudomonas aeruginosa and 35 (28.23%) were Gram-positive including Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Staphylococcus aureus, Staphylococcus haemolyticus and Enterococcus faecalis. Antibacterial assays showed that Bee Venom possesses strong potential effect against MDR isolates including both GNB and GPB. with a wide range of MICs and MLCs concentration-spacing between 3.125-50 μg/mL and 6.25-100 μg/mL, respectively against all MDR-GNB and GPB. It was found that GPB was more sensitive at lower concentrations of Bee Venom than GNB. In addition, Bee Venom sub-MICs values against the most biofilm bacterial produces namely; E. faecalis, P. aeruginosa, S. aureus, VRSA, S. haemolyticus and E. faecalis exhibited sharp reduction in their biofilms ranged between (63.8-92%) especially at ½ MICs according to each bacterium, exclude E. faecalis biofilm was moderately affected (39%). While, at another tested sub-MICs showed moderate, weak, and no antibiofilm effects.

show abstract

Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest

Cited by 43 publications

References 48 publications

Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing

Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing

HIV Drugs Inhibit Transfer of Plasmids Carrying Extended-Spectrum β-Lactamase and Carbapenemase Genes

Antibacterial and Antibiofilm Effects of Bee Venom From (Apis Mellifera) on Multidrug-Resistant Bacteria (Mdrb)

Contact Info

Product

Resources

About