Persistence of a carbapenem-resistant Acinetobacter baumannii (CRAB) International Clone II (ST2/IC2) sub-lineage involved with outbreaks in two Brazilian clinical settings

Fonseca, Érica Lourenço; Morgado, Sérgio Mascarenhas; Freitas, Fernanda; Oliveira, Priscila P.C.; Monteiro, Priscila M.; Lima, Lorena S.; Santos, Bianca P.; Sousa, Maria Aparecida R.; Assunção, Adriana O.; Mascarenhas, Luís Affonso; Vicente, Ana Carolina Paulo

doi:10.1016/j.jiph.2023.08.014

Cited by 2 publications

(2 citation statements)

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“…Most literary data report the ST2 as the crucial carbapenem-resistance OXA gene carrier. According to this evidence, our results perfectly match the global epidemiological condition (Osman et al, 2020;Rezaei et al, 2020;Fonseca et al, 2023). Two analyzed strains revealed the detection of OXA-66, described as an OXA-51 family member (Gupta et al, 2022).…”

Section: Discussionsupporting

confidence: 85%

The molecular detection of carbapenem markers with a two-levels amplification screening protocol: epidemiological and resistome insights

Calvo,

Migliorisi,

Maugeri

et al. 2024

Front. Microbiol.

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ObjectivesCarbapenem-resistance is a challenging healthcare concern and require specific stewardship programs. Monitoring workflows include the identification from surveillance samples, such as rectal swabs. Although culture assays represent the gold standard, data report a significant effectiveness in detecting carbapenemases genes directly from rectal swabs. The aim of this study was to evaluate the REALQUALITY Carba-Screen kit (AB ANALITICA, Padova, Italy) in detecting carbapenemases genes directly from rectal swabs, also comparing its effectiveness to culture assays results. A next-generation sequencing (NGS) was performed to investigate the positive samples about resistance markers and sequence type (ST).MethodsA number of 136 rectal swabs were collected from the University Hospital Policlinico of Catania critical wards. The samples simultaneously underwent culture and molecular assays (REALQUALITY Carba-Screen kit). The molecular method included two-steps. The first step (1 h and 6 min) rapidly excluded negative samples, while the second one (1 h and 6 min) included only positive samples for a resistance confirmation. All the positive culture samples underwent NGS analysis.ResultsStatistical evaluations demonstrated high sensitivity (100%) and detection rates (92.6%) for the REALQUALITY Carba-Screen kit, which mostly correlated to the standard workflow. All the culture positive results matched the positive molecular results, which were mainly confirmed by the NGS resistome analysis. The identified ST appeared to be diversified and different from the clinically significative strains of the same setting, furnishing interesting epidemiological evidence.ConclusionThe molecular detection allowed a coordinate approach in a high-prevalence multi-drug-resistance area. The rapid identification with a multi-step procedure accelerated the infection control procedures, while the preliminary negative results reduced the overtreatment episodes. The molecular method efficacy was confirmed through the NGS. In conclusion, the molecular screening could initially lead to a more conservative approach, which may be reevaluated after a culture result about the microorganisms’ identification and susceptibility profile.

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Section: Discussionsupporting

confidence: 85%

The molecular detection of carbapenem markers with a two-levels amplification screening protocol: epidemiological and resistome insights

Calvo,

Migliorisi,

Maugeri

et al. 2024

Front. Microbiol.

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“…These IC 4 and IC 5 correspond to clonal complexes CC15 Past /CC103 OXF and CC79 Past /CC227 OXF defined by Pasteur ( Past ) and Oxford ( OXF ) Multilocus Sequence Type (MLST) schemes [49][50][51]. Other ICs have been observed in Latin regions, such as IC 1 (CC1 Past / CC109 OXF ), IC 2 (CC2 Past /CC92 OXF ), IC 6 (CC78 Past /CC944 OXF ) and IC7 (CC25 Past / CC110 OXF ) [50,[52][53][54][55].…”

Section: Crab In Latin Americamentioning

confidence: 99%

Carbapenem-resistant Acinetobacter baumannii in Latin America

Pavoni Gomes Chagas,

Rangel,

Giovanni De-Simone

2024

Acinetobacter Baumannii - The Rise of a Resistant Pathogen

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Acinetobacter baumannii is an important bacterial pathogen associated with healthcare-associated infections (HAIs), especially in critically ill patients admitted to Intensive Care Units (ICU). Its ability to acquire antibiotic resistance determinants has propelled its clinical relevance. The rise in Acinetobacter infections and hospital outbreaks have been extensively described worldwide and are usually caused by carbapenem-resistant isolates. To compound the problem, Carbapenem-resistant A. baumannii (CRAb) isolates are also resistant to a wide range of other antibiotics, representing a serious threat to public health. Since 2017, A. baumannii has been listed as a critical priority pathogen that poses a great threat to human health, according to the World Health Organization (WHO). The carbapenem-resistant rates in A. baumannii are notorious around the world. However, Latin America has one of the highest in the world. Carbapenem resistance in A. baumannii is due mainly to the presence of horizontally acquired OXA-type carbapenem resistance genes, including blaOXA-23, in most regions. Thus, this review aims to summarize the distribution of CRAb and its major carbapenem resistance mechanisms in Latin America.

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Persistence of a carbapenem-resistant Acinetobacter baumannii (CRAB) International Clone II (ST2/IC2) sub-lineage involved with outbreaks in two Brazilian clinical settings

Cited by 2 publications

References 36 publications

The molecular detection of carbapenem markers with a two-levels amplification screening protocol: epidemiological and resistome insights

The molecular detection of carbapenem markers with a two-levels amplification screening protocol: epidemiological and resistome insights

Carbapenem-resistant Acinetobacter baumannii in Latin America

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