Persistence and ecological implications of maize-expressed transgenic endo-1,4-β-D-glucanase (E1) in agricultural soils

Abstract

Biofeedstocka plant-based material used in biorefineries as an energy source.Dissipationthe biotic or abiotic inactivation of a biomolecule that prevents detection and functionality.EEC (estimated environmental concentration)a realistic, conservative exposure estimate based on the expression level or concentration (exudate) of the protein, mass of residue or volume loaded, and the applicable quantity of receiving environment (soil).Endogenousproduced from within, native.Enzyme Activity Assaya method for detecting functional enzymes by incubating with a substrate and quantifying some assessment endpoint of catalysis (e.g. colorimetric, dye, fluorogenic, viscosity, mass-balance).Exogenousadded from a foreign source.Mesophiliccharacterized as developing or functioning at moderate environmental temperatures where most organisms exist (generally 20-45 o C).Persistencedetection of a biomolecule over time in a given environmental system (related to dissipation).Thermophilic -"heat-loving"characterized as developing or functioning at high temperatures not sustainable to most organisms (generally 45-120 o C).Transgenican organism that expresses a heterologous gene, often contrasted with traditional breeding.