Persistence and decay of the intestinal microbiota's DNA in glacier mummies from the Alps

Rollo, Franco; Luciani, Stefania; Marota, Isolina; Olivieri, Cristina; Ermini, Luca

doi:10.1016/j.jas.2006.10.019

Cited by 25 publications

(23 citation statements)

References 49 publications

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“…The first studies to extract ancient DNA from coprolites and amplify a portion of the 16S rRNA gene (rDNA) found sequences that were generally consistent with the families and genera expected for gut bacteria. However, because these studies rarely examined more than a few dozen sequence clones per sample (Ubaldi et al, 1998; Cano et al, 2000; Poinar et al, 2001; Luciani et al, 2006; Rollo et al, 2007), there were simply too few data to draw community-level conclusions.…”

Section: Ancient Microbiome Researchmentioning

confidence: 99%

“…However, despite gross preservation at a macroscopic scale, mummified tissues are still susceptible to the same microscopic and molecular taphonomic processes as other ancient tissues, and attempts to date to analyze microbiota from these sources have yielded mixed results. In analyzing the microbiota of the Tyrolean Ice Man, for example, Rollo and colleagues (2000, 2007) found that bacteria collected from the skin and stomach were consistent with environmental contamination, while bacteria obtained from the colon yielded 16S rRNA gene sequences consistent with human gut bacteria. Meanwhile, Castillo-Rojas and colleagues (2008) analyzed gastric samples of a pre-Columbian mummy in Chihuahua, Mexico and successfully PCR-amplified genetic markers for Helicobacter pylori , the causative agent of stomach ulcers.…”

Section: Ancient Microbiome Researchmentioning

confidence: 99%

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Ancient human microbiomes

Warinner

Speller

Collins

et al. 2015

Journal of Human Evolution

135

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Very recently, we discovered a vast new microbial self: the human microbiome. Our native microbiota interface with our biology and culture to influence our health, behavior, and quality of life, and yet we know very little about their origin, evolution, or ecology. With the advent of industrialization, globalization, and modern sanitation, it is intuitive that we have changed our relationship with microbes, but we have little information about the ancestral state of our microbiome, and therefore, we lack a foundation for characterizing this change. High-throughput sequencing has opened up new opportunities in the field of paleomicrobiology, allowing us to investigate the evolution of the complex microbial ecologies that inhabit our bodies. By focusing on recent coprolite and dental calculus research, we explore how emerging research on ancient human microbiomes is changing the way we think about ancient disease and how archaeological studies can contribute to a medical understanding of health and nutrition today.

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Section: Ancient Microbiome Researchmentioning

confidence: 99%

Section: Ancient Microbiome Researchmentioning

confidence: 99%

Ancient human microbiomes

Warinner

Speller

Collins

et al. 2015

Journal of Human Evolution

135

View full text Add to dashboard Cite

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“…Retrieval of sequences belonging to endospore-forming Firmicutes from ancient samples was expected, as Firmicutes endospores are well-known for their persistence under adverse environmental conditions and have been isolated from a variety of materials from hundreds to several thousand years of age (reviewed in Kennedy et al 1994, Rollo et al 2007. Firmicutes have not been found to be abundant in Dry Valley mineral soils (Moodley 2004, Aislabie et al 2006, Smith et al 2006, but are abundant in other Antarctic habitats, including coastal ornithogenic soils (Aislabie et al 2008), microbial mats (Brambilla et al 2001), and sediments (Bowman et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of ancient algal mats from the McMurdo Dry Valleys, Antarctica

Antibus

Leff

Hall³

et al. 2011

Antarctic Science

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Abstract:The McMurdo Dry Valleys possess a cold and dry climate which favours biomolecular preservation, and present the possibility for preservation of biological materials over long timescales. We examined patterns of bacterial DNA abundance and diversity in algal mats from 8-26 539 years of age. Bacterial DNA abundance was inferred from extractable DNA quantity and quantitative polymerase chain reaction targeting the bacterial 16S rRNA gene. Because damage to bacterial DNA could limit its availability for polymerase chain reaction, the efficacy of DNA repair by a commercially available kit was also examined. Polymerase chain reaction amplicons of the bacterial 16S rRNA gene were obtained from seven of eight samples. Bulk DNA abundance and bacterial 16S rRNA gene copy number of template DNA declined with increasing sample age consistent with expectations of accumulation of DNA damage in ancient materials. Clone libraries revealed age related patterns of abundance for some bacterial phylogenetic groups. For example, Firmicutes and several other lineages were abundant in ancient samples, but Cyanobacteria were absent. This points to a biased persistence of bacterial lineages that change over time since photosynthesis was active.

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“…Although most of these bacteria are benign to the host, there are some of bacteria are pathogenic and may cause chronic disorders. 10 Functional oligosaccharides can only be consumed by a limited number of bacteria due to their chemical structure. The bifidobacteria and lactobacilli are considered as the only microorganisms able to benefit the host's health.…”

Section: Definition and Sources Of Functional Oligosaccharidesmentioning

confidence: 99%

Biological Activities and Potential Therapeutic Applications of Functional Oligosaccharides

Lai

Yang

Han

et al. 2017

MOJFPT

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There is an increased interest on researching the functions of oligosaccharides due to their biological activities offer positive effects on our health. The functional oligosaccharides have been widely applied in various branches, such as pharmacological supplements and food ingredients. They are widely found in plants, algae, bacteria, and higher fungi. Pectic oligosaccharides have been regarded as a new kind of prebiotics capable of exerting a number of health-promoting effects, it can protect colonic cells against Shiga toxins, prevent the adhesion of uropathogenic microorganisms, etc. Milk oligosaccharides can promote the growth of the beneficial bacterial flora in the intestines and develop resistance to bacterial and viral infections. Keeping in this view, this article summarized here to discuss the sources, benefit effects, and synthesis of the functional oligosaccharides.

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Persistence and decay of the intestinal microbiota's DNA in glacier mummies from the Alps

Cited by 25 publications

References 49 publications

Ancient human microbiomes

Ancient human microbiomes

Molecular characterization of ancient algal mats from the McMurdo Dry Valleys, Antarctica

Biological Activities and Potential Therapeutic Applications of Functional Oligosaccharides

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