Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway

Zhang, Ge; Zhu, Wei; Peng, Jinming; Deng, Xin; Li, Chunmei

doi:10.1016/j.jff.2016.02.033

Cited by 24 publications

(17 citation statements)

References 44 publications

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“…Cholesterol metabolism is a particularly complex regulatory process, which is the result of multi‐regulation. In the cholesterol efflux pathway, PPAR/LXR/RXR‐ABCA1/ABCG1‐CYP7A1 is one of the most important nuclear receptor signal pathways . Cholesterol‐lowering foods may affect the cholesterol metabolism by decomposing cholesterol into bile acid, regulating transporter factors such as the upstream genes, including liver X receptor (LXR α ), peroxisome proliferator‐activated receptor γ (PPAR γ ) and their downstream genes ATP‐binding cassette transporter A1 (ABCA1), ATP‐binding cassette transporter G1 (ABCG1) and cholesterol 7 α hydroxylase (CYP7A1) .…”

Section: Introductionmentioning

confidence: 99%

Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Luo

Wang

et al. 2019

J Sci Food Agric

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BACKGROUND: Silkworm pupa oil polyunsaturated fatty acid (SPO PUFA) has been confirmed to have a cholesterol-lowering function. METHODS AND RESULTS:The effect of SPO PUFA and its main component, -linolenic acid (ALA), on the metabolism of cholesterol and its regulation was investigated. The model of lipid denatured cells were constructed to carry out lipid accumulation, cholesterol metabolism and transformation. Real-time PCR and western blots were also used to analyze the expression levels of related genes and proteins to investigate the cholesterol efflux regulation mechanism. The data indicated that SPO PUFA and ALA dose-dependently decreased intracellular total cholesterol (TC) and enhanced total bile acid (TBA). They could also promote cholesterol removal by enhancing bile acid secretion and by upregulating genes LXR , PPAR , ABCA1, ABCG1, and CYP7A1, which were regulated by LXR /PPAR -ABCA1/ABCG1-CYP7A1 nuclear receptor signal pathways. CONCLUSIONS: This study is of great significance in maintaining the balance of cholesterol and lipid metabolism, and in reducing the risk of steatohepatitis.The effect of SPO PUFAs and ALA on related gene expression in L02 cells Total cellular RNA was lysed and harvested using the total RNA kit (Omega Bio Tek Inc., Norcross, GA, USA) according to the

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Section: Introductionmentioning

confidence: 99%

Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Luo

Wang

et al. 2019

J Sci Food Agric

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“…As shown in Figure a, pure methanol produced the highest tannin yield among several solvents under the same condition (200 W, 55 °C, 30 min, 25:1 mL:g). Besides, after elaborate purification process, persimmon tannin extracted by methanol with 1% HCl has been applied in mice to explore its anti‐hyperlipidemic effect and regulatory mechanism (Ge et al, ; Zou et al, ), confirming the safety of final product for food and medicinal application. Therefore, methanol was selected as the extraction solvent for both tannin yield and quality.…”

Section: Resultsmentioning

confidence: 99%

“…Distilled water was first used to remove sugar, then ethanol aqueous solution (10%) was used to remove low phenolic compounds. Next, ethanol aqueous solution (95%) was selected to elute objective tannin at a flow rate of 2 mL/min (Ge, Zhu, Peng, Deng, & Li, ). Finally, the eluate was evaporated to near dryness and lyophilized to obtain purified tannin.…”

Section: Methodsmentioning

confidence: 99%

Optimization of ultrasonic-assisted extraction of antioxidant tannin from young astringent persimmon (Diospyros kaki L.) using response surface methodology

Liu

Wang

Yang

et al. 2018

J Food Process Preserv

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Ultrasonic‐assisted extraction (UAE) was applied for tannin extraction from young persimmon (Diospyros kaki L.) and extraction conditions were optimized using response surface methodology to compare with traditional water bath extraction (WBE). The maximum yield (33.96 mg/g) extracted by UAE was obtained under conditions of ultrasonic power 207 W, time 30.9 min, L:S ratio 21.1:1 (mL:g) and 55.0 °C, whereas tannin yield reached 32.39 mg/g under parameters of 73.5 °C, time 63.0 min, and L:S ratio 32.4:1 (mL:g) when using WBE. Comparatively, higher tannin was extracted under optimal UAE condition than WBE method, mainly associated with its great damage on original structure of persimmon pulp and peel based on morphological analysis. While there was no significant difference for UV and FT‐IR spectra among tannin extracted by different methods. Moreover, tannin possessed excellent DPPH radical scavenging and ferric reducing activities in a dosage‐dependent manner, showing potential application in medicinal and food industry. Practical applications Young astringent persimmon possesses abundant functional tannin that is beneficial to human health. Tannin extraction is the most fundamental process for its further research and application. Traditional methods for bioactive compounds extraction are generally conducted with high temperature, large solvent consumption and long time. This study illuminated that ultrasonic‐assisted extraction method had great potential to replace traditional water bath to extract antioxidant tannin from young persimmon.

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“…1) The fruit is highly nutritious, and contains various types of tannins, polyphenols, carotenoids, potassium, vitamins, and dietary fiber. 2,3) Several studies have demonstrated that consumption of persimmon helps to ameliorate various diseases, including cardiovascular disease, 4) diabetes, [5][6][7][8] obesity, [9][10][11][12][13][14] and hypertension. 15) Despite its health-promoting potential, persimmon has several properties that prevent its more widespread consumption.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, fermented legumes have greater antioxidant activity than fresh legumes. 21,22) Although dietary supplementation with persimmon extracts can lower the plasma triglycerides (TGs) and cholesterol levels of laboratory animals with experimentally induced obesity, [9][10][11][12][13][14] the underlying mechanisms are yet to be elucidated. Moreover, the effects of fermented persimmon extracts (FPE) on the metabolic parameters and the underlying mechanisms of these effects are essentially unknown.…”

Section: Introductionmentioning

confidence: 99%

Anti-obesity Effect of Fermented Persimmon Extracts <i>via</i> Activation of AMP-Activated Protein Kinase

Song

Yang

Hoa

et al. 2020

Biological & Pharmaceutical Bulletin

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There is significant cultivation of persimmon (Diospyros kaki) in East Asia, a plant whose fruit has abundant nutrients, including vitamins, polyphenols, and dietary fiber. Persimmon dietary supplements can benefit health by amelioration of diabetes, cardiovascular disease, and obesity. There are also persimmonbased beverages produced via fermentation, such as wines and vinegars, and increasing consumption of these products in East Asia. Although there is great interest in functional foods, the health effects of fermented persimmon extract (FPE) are completely unknown. We examined the effects of FPE on the metabolic parameters of mice fed a high-fat diet (HFD). Our results indicated that FPE supplementation led to an approx. 15% reduction of body weight, reduced abdominal and liver fat, and reduced serum levels of triglycerides, total cholesterol, and glucose. FPE also blocked the differentiation of murine 3T3-L1 pre-adipocyte cells into mature adipocytes. We suggest that gallic acid is a major bioactive component of FPE, and that AMPactivated protein kinase mediates the beneficial effects of FPE and gallic acid.

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Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway

Cited by 24 publications

References 44 publications

Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L‐02

Optimization of ultrasonic-assisted extraction of antioxidant tannin from young astringent persimmon (Diospyros kaki L.) using response surface methodology

Anti-obesity Effect of Fermented Persimmon Extracts <i>via</i> Activation of AMP-Activated Protein Kinase

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