Peroxyl-induced oxidative stress in aging erythrocytes of rat

Devi, S. Asha; Reddy, Chada S.; Subramanyam, M. V. V.

doi:10.1007/s10522-011-9323-x

Cited by 10 publications

(2 citation statements)

References 34 publications

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“…To determine the antioxidant activity of osthole on rat erythrocytes, AAPH was used to form peroxyl radicals and induce oxidation in erythrocytes. 24 Erythrocyte suspensions at 10% hematocrit were preincubated at 37°C for 10 min and subsequently mixed with 200 µl of 25 mM AAPH in PBS (pH 7.4). Then, 80 µl of different concentrations of osthole (25, 50, 100, 200, 400 µM) were added to the tubes.…”

Section: Hemolysis Assaymentioning

confidence: 99%

Protective Effects of Osthole against Free Radical-Induced Hemolysis of Erythrocytes

et al. 2020

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Background: Osthole, one of the most active components of Cnidium monnieri, has different pharmacological and biological effects such as boosting the immune system, reducing rheumatoid pain, hepatoprotective, and inhibitory effect on osteoporosis. Furthermore, it showed anti inflammatory, anti-cancer, and antioxidant properties. However, there is little information about the antioxidant effects of osthole using cell-based assays. In the current work, we used in vitro model of 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes to investigate the protective effects of osthole against oxidative damage of biological membranes. Methods: Erythrocytes were challenged with 2, 2ꞌ-azobis (2-aminopropane) dihydrochloride (AAPH) as a model oxidant in the presence and absence of osthole. The protective effects of osthole on lipid peroxidation, protein carbonyl oxidation, glutathione (GSH) content of erythrocytes were evaluated and compared with control samples. Results: It was found that osthole has protective effects on erythrocyte hemolysis induced by AAPH at different concentrations in a time-dependent manner. Osthole also suppressed lipid and protein oxidation as well as reductions in GSH content in a concentration and timedependent manner. Conclusion: Osthole showed protective effects against free radical-induced hemolysis in rat erythrocytes. Therefore, it can be considered as a supplement for the prevention or treatment of a variety of human health problems associated with oxidative stress. However, further investigations are required to illustrate other possible impacts of osthole on cells.

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Section: Hemolysis Assaymentioning

confidence: 99%

Protective Effects of Osthole against Free Radical-Induced Hemolysis of Erythrocytes

et al. 2020

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“…To induce oxidative hemolysis of erythrocytes, proxy radicals generated by the thermal decomposition of AAPH in oxygen were used [ 12 ]. RBC suspensions (10% hematocrit) were incubated with different concentrations of auraptene (25, 50, 100, 200, and 400 μM) at 37℃ for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Auraptene against Free Radical-Induced Erythrocytes Damage

et al. 2022

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Objectives: Auraptene is the most abundant natural prenyloxycoumarin. Recent studies have shown that it has multiple biological and therapeutic properties, including antioxidant properties. Erythrocytes are constantly subjected to oxidative damage that can affect proteins and lipids within the erythrocyte membrane and lead to some hemoglobinopathies. Due to the lack of sufficient information about the antioxidant effects of auraptene on erythrocytes, this study intended to evaluate the potential of this compound in protecting radical-induced erythrocytes damages. Methods: The antioxidant activity of auraptene was measured based on DPPH and FRAP assays. Notably, oxidative hemolysis of human erythrocytes was used as a model to study the ability of auraptene to protect biological membranes from free radical-induced damage. Also, the effects of auraptene in different concentrations (25-400 µM) on AAPHinduced lipid/protein peroxidation, glutathione (GSH) content and morphological changes of erythrocytes were determined. Results: Oxidative hemolysis and lipid/protein peroxidation of erythrocytes were significantly suppressed by auraptene in a time and concentration-dependent manner. Auraptene prevented the depletion of the cytosolic antioxidant GSH in erythrocytes. Furthermore, it inhibited lipid and protein peroxidation in a time and concentration-dependent manner. Likewise, FESEM results demonstrated that auraptene reduced AAPH-induced morphological changes in erythrocytes. Conclusion: Auraptene efficiently protects human erythrocytes against free radicals. Therefore, it can be a potent candidate for treating oxidative stress-related diseases.

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In‐depth analysis of cysteine oxidation by the RBC proteome: Advantage of peroxiredoxin II knockout mice

et al. 2011

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Peroxiredoxin II (Prdx II, a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Mice lacking Prdx II proteins had heinz bodies in their peripheral blood, and morphologically abnormal cells were detected in the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). In this study, a labeling experiment with the thiol-modifying reagent biotinylated iodoacetamide (BIAM) in Prdx II-/- mice revealed that a variety of RBC proteins were highly oxidized. To identify oxidation-sensitive proteins in Prdx II-/- mice, we performed RBC comparative proteome analysis in membrane and cytosolic fractions by nano-UPLC-MSE shotgun proteomics. We found oxidation-sensitive 54 proteins from 61 peptides containing cysteine oxidation, and analyzed comparative expression pattern in healthy RBCs of Prdx II+/+ mice, healthy RBCs of Prdx II-/- mice, and abnormal RBCs of Prdx II-/- mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cytoskeleton, stress-induced proteins, metabolic enzymes, signal transduction, and transporters. Furthermore, protein networks among identified oxidation-sensitive proteins were analyzed to associate with various diseases. Consequently, we expected that RBC proteome might provide clues to understand redox-imbalanced diseases.

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Peroxyl-induced oxidative stress in aging erythrocytes of rat

Cited by 10 publications

References 34 publications

Protective Effects of Osthole against Free Radical-Induced Hemolysis of Erythrocytes

Protective Effects of Osthole against Free Radical-Induced Hemolysis of Erythrocytes

Protective Effects of Auraptene against Free Radical-Induced Erythrocytes Damage

In‐depth analysis of cysteine oxidation by the RBC proteome: Advantage of peroxiredoxin II knockout mice

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