Peroxisomes, Reactive Oxygen Metabolism, and Stress-Related Enzyme Activities

Rı́o, Luis A. del; Sandalio, Luisa M.; Palma, José M.; Corpas, Francisco J.; López‐Huertas, Eduardo; Romero‐Puertas, María C.; McCarthy, Iva

doi:10.1007/978-94-015-9858-3_7

Cited by 10 publications

(13 citation statements)

References 126 publications

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“…H 2 O 2 is inactivated through the action of catalase which is abundant in peroxisomes (Fig 2). Under conditions of stress, not only can β-oxidation increase in activity, but superoxide, nitric oxide and peroxynitrite are also produced in the peroxisome and these are reported to inhibit catalase activity 24,26 . (Fig 2).…”

Section: Downstream Metabolic Integrationmentioning

confidence: 99%

Chewing the fat: β-oxidation in signalling and development

Baker

Graham

Holdsworth

et al. 2006

Trends in Plant Science

233

195

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Abstract.Peroxisomal β-oxidation is involved not only in fatty acid catabolism and lipid housekeeping but also in metabolism of hormones and amino acids in plants. Recent research in model species has led to new insights into the roles of this pathway in signalling and development, in particular regarding the involvement of β-oxidation in jasmonic acid biosynthesis. Analysis of associated processes, such as the glyoxylate cycle and redox metabolism has also highlighted the importance of integration of β-oxidation with cytosolic and mitochondrial metabolism.Mutations that disrupt β-oxidation can have extremely pleiotropic effects, indicating important and varied roles for this pathway throughout the plant life cycle and making this an exciting topic for future research.2

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Section: Downstream Metabolic Integrationmentioning

confidence: 99%

Chewing the fat: β-oxidation in signalling and development

Baker

Graham

Holdsworth

et al. 2006

Trends in Plant Science

233

195

View full text Add to dashboard Cite

show abstract

“…The distribution of an enzyme system in several organelles indicates that different topogenic signals direct its targeting to different cell compartments, and this is relevant from the viewpoint of the differential regulation of the distinct isozymes under plant stress conditions. Peroxisomes are subcellular organelles bound by a single membrane with an essentially oxidative metabolism (del Río et al ., 2002a,b, 2003). These organelles with their intricate ROS‐machinery and antioxidants, including GR, have been demonstrated to be involved in leaf senescence (del Río et al ., 1998; Jiménez et al ., 1998) and in the response to several abiotic stresses (Romero‐Puertas et al ., 1999; McCarthy et al ., 2001a,b; del Río et al ., 2002b, 2003; Mittova et al ., 2003, 2004).…”

Section: Introductionmentioning

confidence: 99%

Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme

Romero‐Puertas¹,

Corpas²,

Sandalio³

et al. 2006

New Phytol

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The glutathione reductase (GR; EC 1.6.4.2) isozyme present in peroxisomes has been purified for the first time, and its unequivocal localization in these organelles, by immunogold electron microscopy, is reported. • The enzyme was purified c . 21-fold with a specific activity of 9523 units mg − 1 protein, and a yield of 44 µg protein kg − 1 leaves was obtained. The subunit size of the peroxisomal GR was 56 kDa and the isoelectric point was 5.4. The enzyme was recognized by a polyclonal antibody raised against total GR from pea ( Pisum sativum ) leaves.• The localization of GR in peroxisomes adds to chloroplasts and mitochondria where GR isozymes are also present, and suggests a multiple targeting of this enzyme to distinct cell compartments depending on the metabolism of each organelle under the plant growth conditions. • The expression level of GR in several organs of pea plants and under different stress conditions was investigated. The possible role of peroxisomal GR under abiotic stress conditions, such as cadmium toxicity, high light, darkness, high temperature, wounding and low temperature, is discussed.

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“…Peroxisomes are subcellular organelles bound by a single membrane with an essentially oxidative metabolism (del Río et al ., 2002a(del Río et al ., ,b, 2003. These organelles with their intricate ROS-machinery and antioxidants, including GR, have been demonstrated to be involved in leaf senescence Jiménez et al ., 1998) and in the response to several abiotic stresses (Romero-Puertas et al ., 1999;McCarthy et al ., 2001a,b;del Río et al ., 2002bdel Río et al ., , 2003Mittova et al ., 2003Mittova et al ., , 2004. In this work, the purification of peroxisomal GR was carried out for the first time and some of its molecular properties are reported.…”

Section: Introductionmentioning

confidence: 99%

Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme

Romero‐Puertas¹,

Corpas²,

Sandalio³

et al. 2006

New Phytol

Self Cite

150

View full text Add to dashboard Cite

The glutathione reductase (GR; EC 1.6.4.2) isozyme present in peroxisomes has been purified for the first time, and its unequivocal localization in these organelles, by immunogold electron microscopy, is reported. The enzyme was purified c. 21-fold with a specific activity of 9523 units mg(-1) protein, and a yield of 44 microg protein kg(-1) leaves was obtained. The subunit size of the peroxisomal GR was 56 kDa and the isoelectric point was 5.4. The enzyme was recognized by a polyclonal antibody raised against total GR from pea (Pisum sativum) leaves. The localization of GR in peroxisomes adds to chloroplasts and mitochondria where GR isozymes are also present, and suggests a multiple targeting of this enzyme to distinct cell compartments depending on the metabolism of each organelle under the plant growth conditions. The expression level of GR in several organs of pea plants and under different stress conditions was investigated. The possible role of peroxisomal GR under abiotic stress conditions, such as cadmium toxicity, high light, darkness, high temperature, wounding and low temperature, is discussed.

show abstract

Peroxisomes, Reactive Oxygen Metabolism, and Stress-Related Enzyme Activities

Cited by 10 publications

References 126 publications

Chewing the fat: β-oxidation in signalling and development

Chewing the fat: β-oxidation in signalling and development

Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme

Glutathione reductase from pea leaves: response to abiotic stress and characterization of the peroxisomal isozyme

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