Peroxisomes and sexual development in fungi

Peraza-Reyes, Leonardo; Berteaux‐Lecellier, Véronique

doi:10.3389/fphys.2013.00244

Cited by 52 publications

(42 citation statements)

References 135 publications

(230 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peroxisomes also possess specific functions in fungi. They participate in secondary metabolite biosynthesis that produces such as penicillins, AK-toxin and aflatoxin as well as in sexual reproduction (Bartoszewska et al, 2011;Pieuchot and Jedd, 2012;Peraza-Reyes and Berteaux-Lecellier, 2013;Stehlik et al, 2014). Remarkably, the integrity of peroxisomes is important during the infection processes of phytopathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

Chen

Zheng

et al. 2018

Environmental Microbiology

View full text Add to dashboard Cite

Peroxisomal proliferation is highly stimulated during the biosynthesis of mycotoxins and plant infection by Fusarium graminearum. Currently, the functions of the peroxisome in these cellular processes are poorly understood. In this study, we applied genetic, cell biological and biochemical analyses to investigate the functions of the peroxisomes. We constructed targeted deletion of docking machinery components, including FgPex13, FgPex14 and the filamentous fungal specific peroxin FgPex33. Our results indicated that peroxisome dysfunction resulted in a shortage of acetyl-CoA, the precursor of trichothecene biosynthesis, and subsequently decreased deoxynivalenol (DON) production. Deletion mutants of ΔFgPex13, ΔFgPex14 or ΔFgPex33 showed an increased accumulation of endogenous reactive oxygen species (ROS) and reduced phosphorylation of MAP (Mitogen-Activated Protein) kinase FgMgv1. In addition, mutants of the docking peroxin exhibited increased sensitivity toward host oxidative bursts and cell wall integrity stress agents and reduced virulence on host plants. More importantly, we found for the first time that FgPex14 is required for pexophagy in F. graminearum. Overall, our study suggests that peroxisomes play critical roles in DON biosynthesis and virulence in F. graminearum.

show abstract

Section: Introductionmentioning

confidence: 99%

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

Chen

Zheng

et al. 2018

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…In this category, we identified the PEX3 peroxisomal biogenesis factor (Pa_7_8080). The crucial role of β-oxidation and the glyoxylate cycle during sexual development has already been documented [60]. We also found a VelvetA-like-1 protein (VeA, Pa_3_6550) [61].…”

Section: Resultsmentioning

confidence: 62%

“…As such, we identified a putative cruciform DNA recognition protein (Pa_2_440), an ATPase involved in DNA repair (Pa_6_4260), a SIK1-like RNA-binding protein (Pa_5_12950) and the telomere length regulator protein rif1 (Pa_1_3890). In mammals as in yeast, Rif1 is required for checkpoint-mediated cell cycle arrest in response to DNA damage [60].…”

Section: Resultsmentioning

confidence: 99%

A RID-like cytosine methyltransferase homologue controls sexual development in the fungusPodospora anserina

Grognet

Timpano

Carlier

et al. 2019

Preprint

View full text Add to dashboard Cite

21DNA methyltransferases are ubiquitous enzymes conserved in bacteria, plants and 22 opisthokonta. These enzymes, which methylate cytosines, are involved in numerous 23 biological processes, notably development. In mammals and higher plants, methylation 24 patterns established and maintained by the cytosine DNA methyltransferases (DMTs) are 25 essential to zygotic development. In fungi, some members of an extensively conserved 26 fungal-specific DNA methyltransferase class are both mediators of the Repeat Induced Point 27 mutation (RIP) genome defense system and key players of sexual reproduction. Yet, no DNA 28 methyltransferase activity of these purified RID (RIP deficient) proteins could be detected in 29 vitro. These observations led us to explore how RID-like DNA methyltransferase encoding 30 genes would play a role during sexual development of fungi showing very little genomic DNA 31 methylation, if any. 32To do so, we used the model ascomycete fungus P. anserina. We identified the 33 PaRid gene, encoding a RID-like DNA methyltransferase and constructed knocked-out 34ΔPaRid defective mutants. Crosses involving P. anserina ΔPaRid mutants are sterile. Our 35 results show that, although gametes are readily formed and fertilization occurs in a ΔPaRid 36 background, sexual development is blocked just before the individualization of the dikaryotic 37 cells leading to meiocytes. Complementation of ΔPaRid mutants with ectopic alleles of 38 PaRid, including 39 demonstrated that the catalytic motif of the putative PaRid methyltransferase is essential to 40 ensure proper sexual development and that the expression of PaRid is spatially and 41 temporally restricted. A transcriptomic analysis performed on mutant crosses revealed an 42 overlap of the PaRid-controlled genetic network with the well-known mating-types gene 43 developmental pathway common to an important group of fungi, the Pezizomycotina. 44 45 46 3 Author Summary 47Sexual reproduction is considered to be essential for long-term persistence of 48 eukaryotic species. Sexual reproduction is controlled by strict mechanisms governing which 49 haploids can fuse (mating) and which developmental paths the resulting zygote will follow. In 50 mammals, differential genomic DNA methylation patterns of parental gametes, known as 51 'DNA methylation imprints' are essential to zygotic development, while in plants, global 52 genomic demethylation often results in female-sterility. Although animal and fungi are 53 evolutionary related, little is known about epigenetic regulation of gene expression and 54 development in multicellular fungi. Here, we report on a gene of the model fungus Podospora 55 anserina, encoding a protein called PaRid that looks like a DNA methyltrasferase. We 56 showed that expression of the catalytically functional version of the PaRid protein is required 57 in the maternal parental strain to form zygotes. By establishing the transcriptional profile of 58PaRid mutant strain, we identified a set of PaRid direct and/or indirect target genes. Half o...

show abstract

“…In most fungi, β-oxidation of fatty acids and biotin biosynthesis occurs only in peroxisomes. Additionally, peroxisomes are involved in homeostasis of reactive oxygen species and in sexual development [51][52][53].…”

Section: Smnbr1-dependent Pexophagy Occurs In S Macrosporamentioning

confidence: 99%

NBR1 is involved in selective pexophagy in filamentous ascomycetes and can be functionally replaced by a tagged version of its human homolog

et al. 2018

View full text Add to dashboard Cite

Macroautophagy/autophagy is a conserved degradation process in eukaryotic cells involving the sequestration of proteins and organelles within double-membrane vesicles termed autophagosomes. In filamentous fungi, its main purposes are the regulation of starvation adaptation and developmental processes. In contrast to nonselective bulk autophagy, selective autophagy is characterized by cargo receptors, which bind specific cargos such as superfluous organelles, damaged or harmful proteins, or microbes, and target them for autophagic degradation. Herein, using the core autophagy protein ATG8 as bait, GFP-Trap analysis followed by liquid chromatography mass spectrometry (LC/MS) identified a putative homolog of the human autophagy cargo receptor NBR1 (NBR1, autophagy cargo receptor) in the filamentous ascomycete Sordaria macrospora (Sm). Fluorescence microscopy revealed that SmNBR1 colocalizes with SmATG8 at autophagosome-like structures and in the lumen of vacuoles. Delivery of SmNBR1 to the vacuoles requires SmATG8. Both proteins interact in an LC3 interacting region (LIR)-dependent manner. Deletion of Smnbr1 leads to impaired vegetative growth under starvation conditions and reduced sexual spore production under non-starvation conditions. The human NBR1 homolog partially rescues the phenotypic defects of the fungal Smnbr1 deletion mutant. The Smnbr1 mutant can neither use fatty acids as a sole carbon source nor form fruiting bodies under oxidative stress conditions. Fluorescence microscopy revealed that degradation of a peroxisomal reporter protein is impaired in the Smnbr1 deletion mutant. Thus, SmNBR1 is a cargo receptor for pexophagy in filamentous ascomycetes.

show abstract

Peroxisomes and sexual development in fungi

Cited by 52 publications

References 135 publications

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

A RID-like cytosine methyltransferase homologue controls sexual development in the fungusPodospora anserina

NBR1 is involved in selective pexophagy in filamentous ascomycetes and can be functionally replaced by a tagged version of its human homolog

Contact Info

Product

Resources

About