Peroxisome proliferator-activated receptor-γ ligand inhibition of RANTES production by human endometriotic stromal cells is mediated through an upstream promoter element

Peroxisome Proliferator-activated Receptor-γ Down-regulates Chondrocyte Matrix Metalloproteinase-1 via a Novel Composite Element

“…Fig. 2A shows that in the presence of IL-1␤ alone total 35 S-sulfated PG were decreased by 35 Ϯ 10%, from 6.35 Ϯ 0.56 10 4 dpm/10 g DNA in control conditions to 4.12 Ϯ 0.39 10 4 dpm/10 g DNA (p Ͻ 0.01). Addition of Rtz reproducibly induced a dose-dependent stimulation of 35 S-sulfated PG and fully restored control values at doses as low as 1 M, being stable at 10 M. These results show that low concentrations (Յ1 M) of Rtz counteract the detrimental effect of IL-1␤ on proteoglycans production.…”

“…Activities, and MMP1 Production-Chondrocytes were incubated for 18 h in sulfate-free medium in the presence of 35 S-radiolabeled sulfate with or without effectors. 35 S-Sulfate incorporation into cetylpyridinium chloride-precipitable PG was measured in the culture medium.…”

“…35 S-Sulfate incorporation into cetylpyridinium chloride-precipitable PG was measured in the culture medium. Fig.…”

“…Rabbit articular chondrocytes were cultured at high density and incubated for 18 h in 2% FCS/DMEM with or without 10 ng/ml IL-1␤ alone or combined with increasing concentrations of Rtz from 0.5 to 10 M. A, production of total 35 S-radiolabeled proteoglycans. Chondrocytes were cultured as described above.…”

“…Activated PPAR-␥ also acts through DNA binding interaction with its cognate PPRE sequences (direct repeats with single spacing nucleotide, DR1) located in the promoter of target genes. For example, the activated PPAR-␥ inhibits regulated on activation normal T cell expressed and secreted (RANTES) transcription and protein production in endometriotic stromal cells through a specific PPRE at Ϫ334 to Ϫ322 upstream from the RNA polymerase start site (35). We have identified a degenerated DR1 sequence (AAAGCATGAGTCA) located at Ϫ83 to Ϫ71 in the rabbit MMP1 promoter.…”