Peroxisome proliferator activated receptor‐, leptin and tumor necrosis factor‐ mRNA expression during very low calorie diet in subcutaneous adipose tissue in obese women

Bastard, Jean‐Philippe; Hainque, Bernard; Dusserre, Eric; Bruckert, Éric; Robin, D.; Vallier, P.; Perche, Stéphanie; Robin, Pierre; Turpin, G; Jardel, Claude; Laville, Martine; Forest, Claude; Vidal, Hubert

doi:10.1002/(sici)1520-7560(199903/04)15:2<92::aid-dmrr21>3.3.co;2-w

Cited by 25 publications

(38 citation statements)

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“…Therefore, our collective results indicate that many known and novel adipose tissue regulatory factors are indeed expressed by pig adipose tissue where they may be involved in development and metabolism. Several studies have demonstrated a positive association between adipose tissue leptin gene expression and C/EBPa (Miller et al, 1996;Krempler et al, 2000) and PPARg (Bastard et al, 1999) gene expression or C/EBPa protein expression (Chen et al, 1999). In this study, expression of C/EBPa in adipose tissue was not associated with leptin gene expression, whereas expression of PPARg and several other transcription factors were associated with leptin expression.…”

Section: Discussioncontrasting

confidence: 40%

“…Transcription related genes were nearly all positively associated with leptin expression, that is, TCF7L1, nuclear receptor co-repressors (NCOR1), HSF1, PPARg, GTF12, LRP6 and REA, whereas HIPK3 was negatively associated with leptin expression. The positive association of negative acting transcription related regulatory factors, that is, NCOR1, HSF1, GTF12, LRP6 and prohibitin with leptin expression was unexpected and unique, whereas a positive association of PPARg expression with leptin expression has been reported (Bastard et al, 1999).…”

Section: Discussionmentioning

confidence: 96%

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Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Hausman

Barb

Dean

2011

Animal

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The expression of many genes encoding secreted and non-secreted factors have been studied in human and rodent adipose tissue with cDNA microarrays, but few such studies in adipose tissue from growing pigs have been reported. Total RNA was collected at slaughter from outer subcutaneous adipose tissue (OSQ) and middle subcutaneous adipose tissue (MSQ) samples from gilts at 90, 150 and 210 days (n 5 5/age). Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing about 600 pig genes involved in growth and reproduction. Gene expression intensity ratios changed little with age for 100 transcription factors, nuclear receptors, enzymes and other regulatory proteins in OSQ and MSQ from pigs between 90 and 210 days of age. However, the relative expression of 13 genes distinguished OSQ and MSQ depots in growing pigs. The expression of several genes were influenced by age including an increase in CCND3, HSF1 and PTGR1 expression in MSQ and a decrease in UCP2 and REA (prohibitin-2) expression in OSQ. These studies demonstrate for the first time the expression of several key regulatory genes in pig adipose tissue. Simple linear regression analysis showed that leptin gene expression was associated with expression of some of these regulatory genes. Negative associations between expression of some regulatory factors and leptin gene expression indicated that local leptin may decrease or antagonize adipogenesis.

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Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 96%

Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Hausman

Barb

Dean

2011

Animal

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“…[48][49][50][51] There were no clear differences with LC n-3 PUFA supplementation in spite of the known anti-inflammatory effect of LC n-3 PUFA. 52 There are some limitations to this study.…”

Section: Discussionmentioning

confidence: 93%

Additive benefits of long-chain n-3 polyunsaturated fatty acids and weight-loss in the management of cardiovascular disease risk in overweight hyperinsulinaemic women

et al. 2006

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Background: Obesity, inflammation, insulin resistance and cardiovascular disease (CVD) risk are inter-related. Both weight-loss and long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) are independently known to reduce metabolic risk, but the combined effects are unclear.Objective: This study examines whether addition of LC n-3 PUFA to a low fat/high carbohydrate weight-loss programme results in greater improvements in inflammation, insulin sensitivity and CVD risk, than weight-loss alone. Design: One hundred and sixteen overweight insulin-resistant women entered a 24-week randomised intervention study. Thirty-nine women were randomised to a weight-loss programme, with LC n-3 PUFA (WLFO), 38 to a weight-loss programme with placebo oil (WLPO), and 39 to receive placebo oil, with no weight-loss programme (control). Results: Ninety-three women completed the study (35 WLFO, 32 WLPO and 26 control), with significant weight-loss in WLFO (10.871.0%) and WLPO (12.471.0%) compared to the control group (Po0.0001). The WLFO, but not WLPO or control group, showed significant increases in adipose tissue LC n-3 PUFA (0.3470.20 vs 0.1770.10 and 0.1670.10 %DHA, Po0.0001). Weight-loss showed significant improvements in insulin sensitivity (Po0.001), lipid profile (triglycerides Po0.05) and inflammation (sialic acid Po0.05). Time * group effects showed significant decreases in triglycerides (Po0.05) and increases in adiponectin (Po0.01) with LC n-3 PUFA, in the WLFO vs WLPO groups. Conclusions: Weight-loss improved risk factors associated with CVD, with some additional benefits of LC n-3 PUFA on triglycerides and adiponectin. Given the current low dietary intake of LC n-3 PUFA, greater attention should be given to increase these fatty acids in the treatment of obesity.

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“…Lessons learned from the PPARg+/-mouse model, as mentioned above, as well as from clinical observations of obese patients after weight loss, which correlated with reduced PPARg levels, suggest that a moderate decrease in PPARg activity might have beneficial effects on the TG content of WAT, liver and skeletal muscle. In fact, diminution of PPARg transcriptional activity reduces the expression of the lipogenic program and promotes the β-oxidation pathway in the liver and muscle, with an improvement of plasma lipidic parameters as well as insulin sensitivity [179,208,209].…”

Section: Pparg As a Therapeutic Target In Fat Related Diseasesmentioning

confidence: 99%

Fat poetry: a kingdom for PPARγ

2007

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Adipose tissue is not an inert cell mass contributing only to the storage of fat, but a sophisticated ensemble of cellular components with highly specialized and complex functions. In addition to managing the most important energy reserve of the body, it secretes a multitude of soluble proteins called adipokines, which have beneficial or, alternatively, deleterious effects on the homeostasis of the whole body. The expression of these adipokines is an integrated response to various signals received from many organs, which depends heavily on the integrity and physiological status of the adipose tissue. One of the main regulators of gene expression in fat is the transcription factor peroxisome proliferatoractivated receptor g (PPARg), which is a fatty acid-and eicosanoid-dependent nuclear receptor that plays key roles in the development and maintenance of the adipose tissue. Furthermore, synthetic PPARg agonists are therapeutic agents used in the treatment of type 2 diabetes.This review discusses recent knowledge on the link between fat physiology and metabolic diseases, and the roles of PPARg in this interplay via the regulation of lipid and glucose metabolism. Finally, we assess the putative benefits of targeting this nuclear receptor with still-to-be-identified highly selective PPARg modulators.

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Peroxisome proliferator activated receptor‐, leptin and tumor necrosis factor‐ mRNA expression during very low calorie diet in subcutaneous adipose tissue in obese women

Cited by 25 publications

References 0 publications

Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Additive benefits of long-chain n-3 polyunsaturated fatty acids and weight-loss in the management of cardiovascular disease risk in overweight hyperinsulinaemic women

Fat poetry: a kingdom for PPARγ

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