Peroxisome proliferator-activated receptor gamma signaling in human sperm physiology

Liu, Li‐Li; Xian, Hua; Cao, Jingchen; Zhang, Chong; Zhang, Yonghui; Chen, Miaomiao; Qian, Yi; Jiang, Ming

doi:10.4103/1008-682x.150253

“…PPARG has a broad ligand binding spectrum, including unsaturated fatty acids, eicosanoids, and the prostaglandin PGJ 2 43 . In the human testis, PPARG protein is found in Sertoli cells and in germ cells (spermatocytes and spermatozoa) 44 . However, information available in the human Protein Atlas database 45 also indicates interstitial/extratubular PPARG/ Pparg expression.…”

Section: Discussionmentioning

confidence: 99%

Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells

Crespo

¹

,

Assis

²

,

Zhang

³

et al. 2021

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Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig cell-derived growth factor found in all vertebrates. Little information is available regarding Insl3 function in adult spermatogenesis. The Insl3 receptors Rxfp2a and 2b were expressed by type A spermatogonia and Sertoli and myoid cells, respectively, in zebrafish testis tissue. Loss of insl3 increased germ cell apoptosis in males starting at 9 months of age, but spermatogenesis appeared normal in fully fertile, younger adults. Insl3 changed the expression of 409 testicular genes. Among others, retinoic acid (RA) signaling was up- and peroxisome proliferator-activated receptor gamma (Pparg) signaling was down-regulated. Follow-up studies showed that RA and Pparg signaling mediated Insl3 effects, resulting in the increased production of differentiating spermatogonia. This suggests that Insl3 recruits two locally active nuclear receptor pathways to implement pituitary (Fsh) stimulation of spermatogenesis.

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“…The expression of CB1 and PLIN1 in the Shandong black cattle was significantly higher than it was in the Luxi cattle, but the PPARγ2 and HSL expression levels were significantly lower than they were in the Luxi cattle. Previous studies have shown that the rate-limiting enzyme of triacylglycerol (tag) catabolism in hormone-sensitive lipase (HSL) tissue, together with triacylglycerol lipase and monoacylglycerol lipase, regulates the storage of fat in muscle ( Liu et al, 2015 ). PPARγ2 plays an important role in regulating lipid metabolism, adipogenesis, insulin sensitivity, inflammatory response, cell growth and differentiation.…”

Section: Discussionmentioning

confidence: 99%

A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

Liu

¹

,

Liu

²

,

Bai

³

et al. 2021

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Fat metabolism is closely related to the economic characteristics of beef cattle. Therefore, regulating fat deposition and increasing intramuscular fat deposition are among the main goals of breeders. In this study, we aim to explore the regulatory role of CB1 gene on PPARγ2/PLIN1/HSL pathway in fat metabolism, and to further explore the differential expression of regulatory factors of this pathway in Shandong black cattle and Luxi cattle. In this study, CB1 overexpression stimulated lipid synthesis in adipocytes to some extent by increasing the levels of FASN and ACSL1. CB1 inhibitors reduce the lipid content in adipocytes and reduce the expression of GLUT1 and Insig1. In addition, overexpression of CB1 decreased the expression of PPARγ2 and led to an increase in PLIN1 expression and a decrease in HSL expression in adipocytes. We also found that the CB1/PPARγ2/PLIN1/HSL was differentially expressed in the different breeds of cattle and was involved in the regulation of fat metabolism, which affected the fatty acid content in the longissimus dorsi muscle of the two breeds. In short, CB1 participates in lipid metabolism by regulating HSL in the PPARγ2 and PLIN1 pathways, and improves lipid formation in adipocytes. In conclusion, CB1/PPARγ2/PLIN1/HSL pathway may be involved in the regulation of lipid metabolism.

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“…PPARG has a broad ligand binding spectrum, including unsaturated fatty acids, eicosanoids and the prostaglandin PGJ2 41 . In the human testis, PPARG protein is found in Sertoli cells and in germ cells (spermatocytes and spermatozoa) 42 . However, information available in the human Protein Atlas database 43 also indicates interstitial/extratubular PPARG/Pparg expression.…”

Section: Insl3 and Ppargmentioning

confidence: 99%

Insulin-like 3 Affects Zebrafish Spermatogenic Cells Directly and via Sertoli Cells

Crespo

¹

,

Assis

²

,

Zhang

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig cell-derived growth factor found in all vertebrates. Little information is available regarding Insl3 function in adult spermatogenesis. The Insl3 receptors Rxfp2a and 2b were expressed by type A spermatogonia and Sertoli and myoid cells, respectively, in zebrafish testis tissue. Loss of insl3 increased germ cell apoptosis in males starting at 9 months of age, but spermatogenesis appeared normal in fully fertile, younger adults. Insl3 changed the expression of 409 testicular genes. Among others, retinoic acid (RA) signaling was up- and peroxisome proliferator-activated receptor gamma (Pparg) signaling was down-regulated. Follow-up studies showed that RA and Pparg signaling mediated Insl3 effects, resulting in the increased production of differentiating spermatogonia. This suggests that Insl3 recruits two locally active nuclear receptor pathways to implement pituitary (Fsh) stimulation of spermatogenesis.

show abstract

Peroxisome proliferator-activated receptor gamma signaling in human sperm physiology

Cited by 38 publications

References 113 publications

Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells

Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells

A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

Insulin-like 3 Affects Zebrafish Spermatogenic Cells Directly and via Sertoli Cells

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