Peroxidative stress and antioxidant enzymes in children with β-thalassemia major

Ezzat, A.M.; Abdelmotaleb, Ghada S.; Shaheen, Adel; Ismail, Yasser; Diab, Ahmed

doi:10.1097/01.mjx.0000511315.53248.78

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…This study showed a highly significant positive correlation between serum ferritin in thalassemic patients and ALT level (P = 0.001). This was in agreement with Ezzat et al, who found a significant positive correlation between serum ferritin and liver enzymes; AST (r = 0.978, P ˂0.001), and ALT (r = 0.98, P ˂ 0.001) [21]. In our study, 11 patients had IR, two of them had DM, eight patients had IFG, and one patient did not have any glycemic abnormality, suggesting that IR precedes the glycemic abnormalities (prediabetes and DM).…”

Section: Discussionsupporting

confidence: 92%

Evaluation of glycemic abnormalities in children and adolescents with β-thalassemia major

Diab

Abdelmotaleb

Eid

et al. 2021

Egypt Pediatric Association Gaz

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Background The quality of life of B-thalassemia major (β-TM) patients has improved with the use of frequent blood transfusions. However, this leads to chronic iron overload with its sequelae, as prediabetes and diabetes mellitus. This study aimed to assess insulin resistance and glucose abnormalities in a sample of B-thalassemia major patients in Benha, Egypt. Results This case-control study included 40 B-thalassemia major patients on regular blood transfusion and iron chelation. Their ages ranged from 8 to 16 years, and 30 normal age and sex-matched controls. Thorough clinical examination was performed including weight (kg), height (m), body mass index (BMI) (kg/m2), and liver and spleen size. Laboratory investigations were done in the form of complete blood count, liver enzymes, serum ferritin, fasting plasma insulin, and fasting, and 2 h postprandial plasma glucose. Insulin resistance (IR) was calculated using the Homeostasis Model Assessment of insulin resistance (HOMA-IR) index. Insulin resistance was found in 27.5% of thalassemic patients; 18.2% of them had diabetes, 72.7% were prediabetics (with impaired fasting glycemia), and 9.1% had normal fasting and 2 h postprandial plasma glucose level. Insulin resistance increased significantly with increased blood transfusion duration, serum ferritin, liver enzymes, fasting plasma insulin, fasting plasma glucose, and 2 h postprandial plasma glucose (ROC). The curve analysis showed that the duration of blood transfusion, serum ferritin, fasting plasma insulin, fasting, and 2 h postprandial plasma glucose could significantly predict insulin resistance at a certain cut-off point. Conclusion Our data show that HOMA-IR can be used to detect insulin resistance in β-TM patients on long-term blood transfusions, especially patients with high serum ferritin and impaired liver enzymes.

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Section: Discussionsupporting

confidence: 92%

Evaluation of glycemic abnormalities in children and adolescents with β-thalassemia major

Diab

Abdelmotaleb

Eid

et al. 2021

Egypt Pediatric Association Gaz

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“…The absence of plasma MDA increase after transfusion in some subjects might be related to routine supplementation of antioxidants, which could attenuate the rise in MDA levels. A study by Ezzat, et al 23 showed that patients with thalassemia have reduced antioxidant activity and vitamin E levels. Therefore, routine supplementation of vitamin E or other antioxidants should be considered a rational approach.…”

mentioning

confidence: 99%

“…26 The sample size used is larger than similar studies conducted in Pakistan and Egypt. 23,27 Moreover, the novelty of this study is the fact the MDA levels were measured pre-and post-transfusion. This is the first study that employed serial measurements of MDA levels.…”

mentioning

confidence: 99%

Oxidative Stress (Malondialdehyde) in Adults Beta-Thalassemia Major and Intermedia: Comparison Between Before and After Blood Transfusion and Its Correlation with Iron Overload

Atmakusuma¹,

Nasution²,

Sutandyo³

2021

IJGM

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Background: Iron overload is a major problem in both transfusion-dependent (TDT) and non-transfusion-dependent thalassemia (NTDT). It has been known to increase oxidative stress. Meanwhile, blood transfusion as main therapy for thalassemia increases iron overload. One of the markers of oxidative stress is malondialdehyde (MDA). This study aims to provide data on MDA levels in adult thalassemia patients, and to compare the levels before and after transfusion in patients with TDT and NTDT. Methods: This is a cross-sectional, pre-post study in adult patients with thalassemia major and intermedia that received blood transfusion with or without iron-chelating agents in Cipto Mangunkusumo Hospital. Blood samples were taken immediately before the transfusion and one day after. Serum ferritin (SF) assays were conducted by electrochemiluminescence immunoassay method, while transferrin saturation (TS) was calculated by dividing serum iron by the binding capacity. Subsequently, plasma MDA levels assays were performed using the Wills method, and data analysis was conducted using the t-test/Mann-Whitney and Pearson/Spearman correlation test, depending on the data distribution. Results:The 63 respondents recruited consist of 51 TDT and 12 NTDT patients, and their median plasma MDA level before and after transfusion was 0.49 µmol/L and 0.45 µmol/L, respectively. Before transfusion, there was no correlation between SF and MDA, and TS and MDA levels. After the transfusion, there was no correlation between, SF and MDA, or TS and MDA levels. Conclusion:There is no significant difference in MDA levels before and after transfusion. Although blood transfusion increases the iron load in thalassemia patients, there was no increase in median MDA level after transfusion. Meanwhile, there was no correlation between markers of iron overload and MDA level in thalassemia patients both before and after transfusion.

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Effects of three months of treatment with vitamin E and N-acetyl cysteine on the oxidative balance in patients with transfusion-dependent β-thalassemia

et al. 2020

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Peroxidative stress and antioxidant enzymes in children with β-thalassemia major

Cited by 3 publications

References 9 publications

Evaluation of glycemic abnormalities in children and adolescents with β-thalassemia major

Evaluation of glycemic abnormalities in children and adolescents with β-thalassemia major

Oxidative Stress (Malondialdehyde) in Adults Beta-Thalassemia Major and Intermedia: Comparison Between Before and After Blood Transfusion and Its Correlation with Iron Overload

Effects of three months of treatment with vitamin E and N-acetyl cysteine on the oxidative balance in patients with transfusion-dependent β-thalassemia

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