Peroxidative Reactions in Red Cell Biology

Chiu, Danny; Lubin, Bertram H.; Shohet, Stephen B.

doi:10.1016/b978-0-12-566505-6.50012-0

Cited by 138 publications

(85 citation statements)

References 175 publications

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“…The reaction of a superoxide radical with peroxides in the erythrocytes produces highly reactive intermediates, such as the hydroxyl radical ('OH). These radicals in turn react with the lipid and protein components of the membrane, damaging its integrity and leading to eventual hemolysis of the cell (40). Lipid peroxidation in the erythrocyte membrane can result in accumulation of an aldehyde, which can cause a reduction in deformability (43,44) and formation of irreversibly sickled cells (45).…”

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confidence: 99%

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Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Kay¹,

Bosman²,

Shapiro³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

101

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Senescent-cell antigen is a "neo-antigen" that appears on the surface of senescent cells and initiates IgG binding and cellular removal. As an approach to evaluating oxidation as a possible mechanism for generation of senescentcell antigen, we studied erythrocytes from vitamin E-deficient rats. Vitamin E is localized primarily in cellular membranes. Its major role is the termination of free-radical chain reactions propagated by the polyunsaturated fatty acids of membrane phospholipids. Results of our studies indicate that erythrocytes of all ages from vitamin E-deficient rats behave like old erythrocytes from normal rats, as determined by their susceptibility to phagocytosis, IgG binding, anion transport ability, and glyceraldehyde-3-phosphate dehydrogenase activity. Increased breakdown products of band 3 were observed with immunoblotting in membranes of erythrocytes from vitamin E-deficient rats. Breakdown products of band 3 are known to increase as cells age in normal individuals. The data suggest that oxidation may be a possible mechanism for erythrocyte aging and generation of senescent-cell antigen in vivo.

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mentioning

confidence: 99%

“…Hemoglobin is known to catalyze lipid peroxidation as well as enhance the decomposition of lipid hydroperoxides to the corresponding free radicals (40). Autooxidation of oxyhemoglobin to methemoglobin results in the generation ofa superoxide radical (41,42).…”

mentioning

confidence: 99%

Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Kay¹,

Bosman²,

Shapiro³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

101

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“…Moreover, we also considered that much of the difference between hemolysis induced by Al-gel and by Ca-gel might be due to membrane oxidation caused by adjuvant gels. Because of the abundance of polyunsaturated fatty acids on those membranes, erythrocytes are very susceptible to peroxidation ( 1). Actually, we found that such radical scavengers as 4-aminosalicylic acid and 5-aminosalicylic acid inhibited Ca-gel-induced hemolysis but not Al-gelinduced hemolysis (unpublished data).…”

Section: Discussionmentioning

confidence: 78%

Relationship between Hemolytic Activity and Adsorption Capacity of Aluminum Hydroxide and Calcium Phosphate as Immunological Adjuvants for Biologicals

Katô

Shibano

Saito

et al. 1994

Microbiology and Immunology

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Aluminum hydroxide (Al) and calcium phosphate (Ca) gels have been used as vaccine adjuvants for many years. We investigated mechanism of the hemolytic activities of both adjuvant materials. The hemolytic activity of each gel depended on the gel dose. The adsorption capacities and the hemolytic activities of both adjuvants decreased as the concentration of phosphate increased in a gel-washing solution. A positive correlation between the hemolytic activity and the adsorption capacity was found in Al-gel. A disruptive effect of Ca-gel on membrane of erythrocytes was shown by electron microscopy. Ca-gel required more than 10 times as much pre-adsorbed ovalbumin as did Al-gel to inhibit the hemolysis. These results suggest that the hemolytic activity of both adjuvant materials depended mainly on the adsorption ability, and it may be useful to control the adsorption ability of adjuvants to reduce their hemolytic activity.

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“…Again, red cells are always under high oxygen pressure and extremely susceptible to peroxidation and the conditions that favour peroxidation, are seemingly optimal in red cells [10]. Noble et al reported that the concentrations of total fatty acids and free cholesterol in the plasma of calves are decreased following short-term exposure to heat [11].…”

Section: Introductionmentioning

confidence: 99%

Studies on Certain Heat Induced Changes of Erythrocyte Membrane with Supplementation of Ascorbic Acid and Alteration of Dietary Protein Level

Ghosh¹

2017

IJRASET

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The present study was aimed to determine the effect of ascorbic acid on certain changes of erythrocyte membrane of heat exposed rat, fed on altered dietary protein levels. Elevated cholesterol to phospholipid (C/PL) ratios (7.56 ±0.26; p<0.001 & 10.22 ±0.36; p<0.001) in erythrocyte membrane were found both adequately (18%) protein-fed rats and protein-restricted (6%) rats respectively, following chronic exposure to heat. Prior supplementation with ascorbic acid potentiated the effect of chronic heat exposure on cholesterol content (58.47±0.98; p<0.01& 58.38 ±1.35; p<0.001) of erythrocyte membrane of both adequately protein-fed rats and protein-restricted rats respectively, while it tended to restore partially phospholipid content (7.65 ±0.57; p<0.05) of the erythrocyte membrane in adequately protein-fed rats only. The increased activity of AchE was observed, unlike ATPases which showed depressed activity. Significant decrease in iodine number and increase in conjugated diene formation and increased alanine liberation were found in erythrocyte membrane of chronic heat exposed rat. Acute exposure showed the elevated formation of conjugated diene (4.96 ±0.13; p a <0.01) in rats fed a 6% protein diet and decreased in iodine number (36.39 ±1.09; p a <0.05) in adequately protein-fed rats only.

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Peroxidative Reactions in Red Cell Biology

Cited by 138 publications

References 175 publications

Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Relationship between Hemolytic Activity and Adsorption Capacity of Aluminum Hydroxide and Calcium Phosphate as Immunological Adjuvants for Biologicals

Studies on Certain Heat Induced Changes of Erythrocyte Membrane with Supplementation of Ascorbic Acid and Alteration of Dietary Protein Level

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