Peroxidases Produced by New Ligninolytic
Bacillus strains Isolated from Marsh and Grassland
Decolourized Anthraquinone and Azo Dyes

A B S T R A C TThis study sought to determine the process conditions for optimum peroxidase production by a Bacillus species (Bacillus sp. FALADE-1-KX640922) isolated from Hogsback forest reserve in South Africa and characterize the peroxidase gene in the bacteria. We optimized peroxidase production by manipulating the environmental and nutritional parameters under submerged fermentation. Subsequently, the gene encoding heme-peroxidase was determined through nested polymerase chain reaction and Sanger DNA sequencing. The studied bacteria had maximum peroxidase production at pH 8, 30 C and 150 rpm. The addition of guaiacol to lignin fermentation medium enhanced peroxidase production by over 100 % in the studied bacteria. However, the other lignin monomers (veratryl alcohol, vanillin, vanillic acid and ferulic acid) repressed the enzyme activity. Modification of the fermentation medium with ammonium sulphate gave the maximum peroxidase yield (8.87 U mL À1 ). Under the predetermined culture conditions, Bacillus sp. FALADE-1 expressed maximum specific peroxidase activity at 48 h (8.32 U mg À1 ). Interestingly, a search of the sequenced gene in PeroxiBase showed 100% similarity to Sporotrichum thermophile catalase-peroxidase gene (katG), as well, the deduced protein sequence clustered with bacterial catalase-peroxidases and had a molecular weight of about 11.45 kDa with 7.01 as the estimated isoelectric point. Subsequently, the nucleotide sequence was deposited in the National Center for Biotechnology Information (NCBI) repository with the accession number MF407314. In conclusion, Bacillus sp. FALADE-1 is a promising candidate for improved peroxidase production.

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“…Subsequently, the bacteria was identified as a Bacillus sp. using 16S rDNA partial sequence analysis (Falade et al., 2019a).…”

Section: Methodsmentioning

confidence: 99%

Studies on peroxidase production and detection of Sporotrichum thermophile-like catalase-peroxidase gene in a Bacillus species isolated from Hogsback forest reserve, South Africa

Falade

Mabinya

Okoh

et al. 2019

Heliyon

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“…determined showed peroxidase activity, with the highest potential for peroxidase production exhibited with 6.53 U mL −1 . The obtained results rendered the isolated ligninolytic Bacillus species as promising potentials for peroxidase production [53] . The ligninolytic potential of the isolates was further confirmed by assessing their decolorizing abilities of two ligninolytic indicator dyes, RBBR and CR, with different arene substituent attachment positions.…”

Section: Recent Discoveries Of Peroxidase Isoforms From Different Soumentioning

confidence: 93%

“…The ligninolytic potential of the isolates was further confirmed by assessing their decolorizing abilities of two ligninolytic indicator dyes, RBBR and CR, with different arene substituent attachment positions. Out of 13 isolates, 5 isolates showed 38.46% decolorization of RBBR while 11 isolated showed up to 84.62% decolorization of CR within 72 hours of incubation [53] .…”

Section: Recent Discoveries Of Peroxidase Isoforms From Different Soumentioning

confidence: 98%

“…Various ligninolytic bacteria were isolated from marsh and grassland in Hogsback located in Raymond Mhlaba Municipality, Eastern Cape, South Africa [53] . The isolates were presumed to be ligninolytic due to their ability to utilize alkali lignin as the sole carbon source in an enrichment medium.…”

Section: Recent Discoveries Of Peroxidase Isoforms From Different Soumentioning

confidence: 99%

“…They are promising candidates for bioremediation, and delignification of feedstock for production of bioethanol. They play a huge role in the valorization of lignocellulosic biomass into other value-added products [53] .…”

Section: Recent Discoveries Of Peroxidase Isoforms From Different Soumentioning

confidence: 99%

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Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin

et al. 2020

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Peroxidases are classified as oxidoreductases and are the second largest class of enzymes applied in biotechnological processes. These enzymes are used to catalyze various oxidative reactions using hydrogen peroxide and other substrates as electron donors. They are isolated from various sources such as plants, animals and microbes. Peroxidase enzymes have versatile applications in bioenergy, bioremediation, dye decolorization, humic acid degradation, paper and pulp, and textile industries. Besides, peroxidases from different sources have unique abilities to degrade a broad range of environmental pollutants such as petroleum hydrocarbons, dioxins, industrial dye effluents, herbicides and pesticides. Ironically, unlike most biological catalysts, the function of peroxidases varies according to their source. For instance, manganese peroxidase (MnP) of fungal origin is widely used for depolymerization and demethylation of lignin and bleaching of pulp. While, horseradish peroxidase of plant origin is used for removal of phenols and aromatic amines from waste waters. Microbial enzymes are believed to be more stable than enzymes of plant or animal origin. Thus, making microbially-derived peroxidases a well-sought-after biocatalysts for versatile industrial and environmental applications. Therefore, the current review article highlights on the recent breakthroughs in the discovery and use of peroxidase isoforms of microbial origin at a possible depth.

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Mixed Consortium of Salt-Tolerant Phosphate Solubilizing Bacteria Improves Maize (Zea mays) Plant Growth and Soil Health Under Saline Conditions

Chauhan

Upadhyay

2023

Mol Biotechnol

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Peroxidases Produced by New Ligninolytic Bacillus strains Isolated from Marsh and Grassland Decolourized Anthraquinone and Azo Dyes

Cited by 13 publications

References 27 publications

Studies on peroxidase production and detection of Sporotrichum thermophile-like catalase-peroxidase gene in a Bacillus species isolated from Hogsback forest reserve, South Africa

Studies on peroxidase production and detection of Sporotrichum thermophile-like catalase-peroxidase gene in a Bacillus species isolated from Hogsback forest reserve, South Africa

Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin

Mixed Consortium of Salt-Tolerant Phosphate Solubilizing Bacteria Improves Maize (Zea mays) Plant Growth and Soil Health Under Saline Conditions

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