Peroxidase mimics of platinum-group metals for in vitro diagnostics: opportunities and challenges

Abstract

Platinum-group metal (PGM) nanostructures with peroxidase-like catalytic activities (i.e., peroxidase mimics) have been actively developed and applied to in vitro diagnostics in recent years. This article provides our viewpoints on...

Bio-conjugated carbon dots for the bimodal detection of prostate cancer biomarkers via sandwich fluorescence and electrochemical immunoassays

“…33 In HRP, the active site heme is buried deep in the polypeptide macromolecules, which forms an insulating layer and eventually impedes direct electron transfer between the enzyme and electrode. 34,35 However, freely diffusing electron transfer mediators, such as hydroquinone, can shuttle electrons between the active site of the enzyme and the electrode surface during the electro-catalytic reaction between HRP and H 2 O 2 . 36 Hence, to elaborate on the role of the mediator, the electro-catalytic property of HRP-modified OCDs (OCDs@HRP) over PNP SPE (SPE-PNPs-OCDs@HRP) was evaluated by cyclic voltammetry in the presence of H 2 O 2 with and without redox mediator HQ (Fig S5, ESI†).…”

Iridium Nanoparticles as Highly Effective Peroxidase Mimics: Synthesis, Characterization, and Application in Biosensing

“…[2a,5] Because peroxidases are responsible for the production of detection signal, they greatly determine the performance of a diagnostic kit, including detection sensitivity and reproducibility. [6] Nevertheless, the inherent drawbacks of peroxidases, such as poor stability, low catalytic efficiency, and high cost of isolation and purification, [4,7] significantly restrict further advancement of associated diagnostic technologies.…”

“…Among various peroxidase mimics, those made of platinumgroup metals (e. g., Pd, Pt, Ir, and Rh) have drawn increasing attention because of their outstanding catalytic efficiencies. [6,12] In a recent study, we have systematically compared the catalytic efficiencies of peroxidase mimics made of various platinumgroup metals. [13] We found that Ir is the most catalytically active one among all other platinum-group metals.…”