Peroxidase‐Mimicking Nanozyme with Enhanced Activity and High Stability Based on Metal–Support Interactions

Abstract

Peroxidase-mimicking nanozymes offer unique advantages in terms of high stability and low cost over natural peroxidase for applications in bioanalysis, biomedicine, and the treatment of pollution. However, the design of high-efficiency peroxidase-mimicking nanozymes remains a great challenge. In this study, we adopted a structural-design approach through hybridization of cube-CeO and Pt nanoparticles to create a new peroxidase-mimicking nanozyme with high efficiency and excellent stability. Relative to pure cube-CeO and Pt nanoparticles, the as-hybridized Pt/cube-CeO nanocomposites display much improved activities because of the strong metal-support interaction. Meanwhile, the nanocomposites also maintain high catalytic activity after long-term storage and multiple recycling. Based on their excellent properties, Pt/cube-CeO nanocomposites were used to construct high-performance colorimetric biosensors for the sensitive detection of metabolites, including H O and glucose. Our findings highlight opportunities for the development of high-efficiency peroxidase-mimicking nanozymes with potential applications such as diagnostics, biomedicine, and the treatment of pollution.

Tuning Atomically Dispersed Fe Sites in Metal–Organic Frameworks Boosts Peroxidase-Like Activity for Sensitive Biosensing

“…Recently, nanozymes, a series of nanomaterials with enzyme-mimicking characteristics, have been selected as the substitutes for natural enzymes and applied in various fields because of their high stability, low-cost and mass production [7][8][9][10][11]. However, the catalytic activities of nanozymes are still much lower than those of natural enzymes [12][13][14]. Given the electronic and geometrical structures of enzymes are two key factors toward catalytic activities, accurately designing and tuning the electronic and geometrical structure of active sites at the atomic scale are important for the development of advanced nanozymes [15][16][17][18].…”

CeO2‐Encapsulated Hollow Ag–Au Nanocage Hybrid Nanostructures as High‐Performance Catalysts for Cascade Reactions

“…The peaks at 2θ = 28.5°, 47.4°, and 56.4° can be ascribed to the characteristic (111), (220), and (311) reflections of face‐centered cubic structure of CeO 2 nanocrystals (JCPDS No. 43‐1002) …”

“…Used Ag–Au NC‐30@CeO 2 as an example, the doublets ( V 0 , U 0 ), ( V 2 , U 2 ), and ( V 3 , U 3 ) were identified as the different states of Ce 4+ species, while the doublets ( V 1 , U 1 ) were identified as the Ce 3+ ions . Besides, the two peaks of 83.2 and 86.9 eV were correspond to the Au 4f 7/2 and 4f 5/2 spin–orbit peaks and the doublets of 367.4 and 373.3 eV were index to Ag 3d 5/2 and 3d 3/2 spin–orbit peaks, respectively (Figure D) . However, in the full XPS spectra of Ag–Au NC‐30@CeO 2 , the signals of both Au and Ag were weak (Figure A).…”

“…Since Ag–Au NC@CeO 2 core–shell structures were composed of different catalytic active species, in which nano‐Au had GOx‐like activity and all the element possessed peroxidase‐like activity . We considered that Ag–Au NC@CeO 2 may owned by the ability of catalyzing multistep reaction.…”

Sensitive Colorimetric Assay Based on Peroxidase‐Like Activity of CeO2 Nanoparticles Supported on SBA‐15 Mesoporous Silica to Determination of H2O2

“…The authors verified that the nanoceria was cooperatively modulated with the aid of adenosine triphosphate and results demonstrates the improving the analytical performance of such self‐regulated bioassays. Li et al . verified how different structure of CeO 2 NPs (cube and nanorods) combinates with metal nanoparticles can be improve the catalytic activity to oxidation TMB by H 2 O 2 decomposition.…”