Peroxidase-Linked Assay for Detection of Antibodies Against Bovine Leukosis Virus

Castro, Clarissa Caetano de; Nunes, Cristina Freitas; Finger, Paula Fonseca; Siedler, Bianca Sica; Dummer, Luana Alves; Lima, Marcelo de; Fischer, Geferson; Vargas, Gilberto D’Ávila; Hübner, Sílvia de Oliveira

doi:10.1080/15321819.2012.747442

Journal of Immunoassay and Immunochemistry

2013

DOI: 10.1080/15321819.2012.747442

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Peroxidase-Linked Assay for Detection of Antibodies Against Bovine Leukosis Virus

Clarissa Caetano de Castro

Cristina Freitas Nunes

Paula Fonseca Finger

et al.

Abstract: A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate tha… Show more

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Evaluation of a Voluntary Control Program for the Detection of Bovine Leukemia Virus Antibodies Based on Agar Gel Immunodiffusion Test in Dairy Farms in Costa Rica

Dolz¹,

Huijsen²,

Jiménez³

et al. 2015

OJVM

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Cattle from 20 dairy farms were serologically tested over a five-year period using agar gel immunodiffusion test (AGIDT) as part of a voluntary Bovine Leukemia Virus (BLV) control program. After five years of removing infected animals from the herds based on BLV-AGIDT serological status, blood samples from 332 cattle in these farms were collected and analyzed side by side by AGIDT and enzyme-linked immunosorbent assay (ELISA) to detect antibodies against BLV. AGIDT detected 29.2% (97) and 16.0% (53) of the animals as positive and weak positive respectively, whereas ELISA detected 58.2% (193) cattle as positive. The prevalence of BLV-antibodies determined with AGIDT in the dairy farms oscillated between 0% and 86%, whereas prevalence determined by ELISA ranged between 28% and 100% in the same farms. Although both techniques showed similarly results in farms with high BLV-prevalence, ELISA detected a larger proportion of BLVpositive, especially in farms with low or no BLV-prevalence based on AGIDT, leading to wrong assumptions in terms of farm level control efforts. Our results strongly suggest that AGIDT alone is inadequate to implement BLV control programs and ELISA is a more adequate test for BLV surveillance and control programs.

show abstract

Evaluation of a Voluntary Control Program for the Detection of Bovine Leukemia Virus Antibodies Based on Agar Gel Immunodiffusion Test in Dairy Farms in Costa Rica

Dolz¹,

Huijsen²,

Jiménez³

et al. 2015

OJVM

View full text Add to dashboard Cite

show abstract

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Peroxidase-Linked Assay for Detection of Antibodies Against Bovine Leukosis Virus

Cited by 1 publication

References 19 publications

Evaluation of a Voluntary Control Program for the Detection of Bovine Leukemia Virus Antibodies Based on Agar Gel Immunodiffusion Test in Dairy Farms in Costa Rica

Evaluation of a Voluntary Control Program for the Detection of Bovine Leukemia Virus Antibodies Based on Agar Gel Immunodiffusion Test in Dairy Farms in Costa Rica

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