Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

Flott, Berenike E.; Moerschbacher, Bruno M.; Reisener, H. J.

doi:10.1111/j.1469-8137.1989.tb00704.x

Cited by 52 publications

(28 citation statements)

References 22 publications

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“…2). These results were in agreement with those obtained by Flott et al (1989) and Mohammadi and Kazemi (2002), who reported an increase in POX activity in susceptible wheat plants (cv. Falat) after inoculation with F. graminearum in the milk stage.…”

Section: Antioxidant Enzyme Activity In the Host Plantssupporting

confidence: 83%

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Gherbawy¹,

El-Tayeb²,

Maghraby

et al. 2012

Acta Agronomica Hungarica

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Inoculating wheat (cv. Giza 164) with various Fusarium species had different effects on the catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) activity in different plant tissues. The CAT activity induced in the shoots of wheat plants by some pathogens (F. merismoides, F. moniliforme, F. poae and F. sambucinum) was relatively higher than that induced by other pathogens. The results showed an increase in POX activity in infected shoots except those inoculated with F. sambucinum, where the POX activity decreased. Inoculating wheat plants with individual Fusarium species or with a mixture of pathogens caused an increase in APX in the shoots, except for those treated with F. poae, where the APX activity decreased. The activity of these antioxidant enzymes thus increased in most cases in the shoot system of wheat plants under Fusarium infection. Investigations on the metabolic activity of wheat plants inouculated with individual Fusarium species or with a mixture of pathogens showed higher contents of all the protein fractions, soluble sugars, total free amino acids and proline. The K + and Ca 2+concentrations decreased to various extents in different cases. The Na + content increased in wheat shoots, especially after inoculation with F. merismoides. The results signify that the most serious infection was caused by F. culmorum, F. graminearum and F. oxysporum and by a mixture of all the Fusarium species.

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Section: Antioxidant Enzyme Activity In the Host Plantssupporting

confidence: 83%

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Gherbawy¹,

El-Tayeb²,

Maghraby

et al. 2012

Acta Agronomica Hungarica

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“…Increases in activities of PO-As have been correlated with resistance in both dicots (25) and moncots (5). In both of these cases, lignification was thought to have a role in the defense response.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in PO activity has been associated with infection in numerous studies (5,6,17,26,33), and some attribute this to a general resistance response (16,(23)(24)(25)30). However, only a few reports link the accumulation of PO isoenzymes to race-specific resistance (17,26,33).…”

Section: Discussionmentioning

confidence: 99%

“…Although a role for POs in defense responses has not been clearly demonstrated, increases in PO activity have been correlated with infection in many species, including cotton (16,33), cucurbits (25), rice (26,30), and wheat (5,17,23,24). For example, after infection of cotton 'Abbreviations: PO, peroxidase; AEC, 3-amino-9-ethylcarbazole; APS, ammonium persulfate; CA, coniferyl alcohol; G6P-D, glucose-6-phosphate dehydrogenase; IEF, isoelectric focusing; PO-A, anionic peroxidase; PO-C, cationic peroxidase; pl, isoelectric point; anti-HRP, anti-horseradish peroxidase.…”

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confidence: 99%

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Increased Activity of a Cationic Peroxidase Associated with an Incompatible Interaction Between Xanthomonas oryzae pv oryzae and Rice (Oryza sativa)

Reimers¹,

Guo²,

Leach³

1992

Plant Physiol.

109

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Rice (Oryza sativa L.) cultivar Cas 209 carries the gene Xa-10 for resistance to race 2 of Xanthomonas oryzae pv oryzae, the bacterial blight pathogen. When seedling leaves of Cas 209 plants were infiltrated with bacterial cell suspensions of strain PX086RIf (race 2, incompatible), total peroxidase activity in extracts from extracellular spaces increased almost threefold between 16 and 24 hours after inoculation. The increase in total peroxidase activity in extracellular extracts was correlated with the appearance of a 43-kilodalton peroxidase isoenzyme with an isoelectric point of 8.6. Increases in the activities of two anionic peroxidase isoenzymes also were associated with the incompatible interaction. Later during the interactions, total peroxidase activities increased in both compatible (cv Cas 209 infiltrated with race 1, PXO61sm) and control (Cas 209 infiltrated with water) treatments, but final activity levels were less than that observed in the incompatible combination. Similarly, the cationic peroxidase was detected in all three treatments by 48 hours after infiltration, but at reduced levels in compatible and water-infiltrated control treatments relative to the incompatible combination. Accumulation of this peroxidase in extracellular spaces thus may play a role in the defense response in cultivar Cas 209. Rice (Oryza sativa L.) is one of the most important cereal crops worldwide, sustaining more than one-half of the global population (20). Like many plant species, rice employs a diverse array of defenses that minimize losses during pathogen attack. For example, melanin deposition and coordinated increases in peroxidase activities and phenolic compounds are associated with the colonization of rice leaves by Magnaporthe grisea, the blast pathogen (26, 30). Phenolic compounds toxic to Xanthomonas oryzae pv oryzae, the causal agent of bacterial blight, also are found in greater amounts in healthy leaves of resistant cultivars compared to healthy leaves of susceptible cultivars (9). Phytoalexins are thought

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“…Our studies indicate that suberization might be important in resistance. Higher levels of peroxidase activities have been associated with resistance to other pathogens (10,12,22,26,29). The nature of the reactions such peroxidases catalyze are not known.…”

Section: Temporal Induction Of Peroxidase Mrna In Tomato Cell Culturesmentioning

confidence: 99%

Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum

Mohan¹,

Kolattukudy²

1990

Plant Physiol.

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We tested whether the expression of the suberization-associated anionic peroxidase gene is involved in the timely appearance of the vascular suberized coating involved in the resistance of a tomato line to Verticillium albo-atrum. The mRNA for this peroxidase appeared at a higher level one day earlier in wound-healing fruits of the resistant tomato line than in a near-isogenic susceptible line. Cell cultures from the resistant line, when treated with low levels (nanograms per milliliter) of fungal elicitor, generated the peroxidase mRNA and this apparent activation of the peroxidase gene expression could be detected in minutes, whereas the cells from the susceptible line hardly responded. possess inherent differences in their ability to respond to the fungal attack. Resistance, which was found to be from a single dominant Mendelian gene (25), was shown to be associated with a timely deposition of vascular coating on the walls of the infected cells (27). The chemical composition of this vascular coating showed it to be suberin ( 18). Since suberization has been found to be regulated by a highly anionic peroxidase (17,24), it appeared possible that the resistance response might involve induction of this suberization-associated peroxidase, and the two cell lines might differ in their ability to respond to fungal signals by triggering the peroxidase gene expression. In this report, we show that wounding and fungal elicitors induce the peroxidase mRNA earlier and to a higher level in the resistant tomato line than in the susceptible line.

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Peroxidase isoenzyme patterns of resistant and susceptible wheat leaves following stem rust infection

Cited by 52 publications

References 22 publications

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infections

Increased Activity of a Cationic Peroxidase Associated with an Incompatible Interaction Between Xanthomonas oryzae pv oryzae and Rice (Oryza sativa)

Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum

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