Peroxidase immobilized on acrylic copolymer beads

Cremonesi, P.; Mazzola, G.; Focher, B.; Vecchio, Giuseppe

doi:10.1002/apmc.1975.050480102

Cited by 8 publications

(2 citation statements)

References 7 publications

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“…The oxidation leads to the presence of one more characteristic band of OCMC in 880 cm -1 regions. The broad band at 880 cm -1 can be assigned to the hemiacetal and hydrated form of the dialdehyde cellulose [21][22][23][24][25][26][27][28][29], the sharp band at 1,728 cm -1 is characteristic of carbonyl groups stretching, bands at 2,928 and 2,718 cm -1 show the C-H stretching of aldehyde group. FT-IR analysis of both CMC and OCMC is complicated owing to the high affinity of the samples with water.…”

Section: Oxidation Of Carboxymethyl Cellulosementioning

confidence: 99%

“…Periodate oxidation is a highly specific reaction to convert 1,2-dihydroxyl (glycol) groups to paired aldehyde groups without significant side reactions and is widely used in structural analysis of carbohydrates [16][17][18][19][20][21][22]. When applied to glucose in the carboxymethyl cellulose chain, this reaction cleaves the C2-C3 bond, according to the mechanism of Malaprade reaction [23,24]. The resulting compound is the dialdehyde cellulose (DAC) [25,26].…”

Section: Introductionmentioning

confidence: 99%

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Structural characterization of chitosan and oxidized carboxymethyl cellulose based freeze-dried films

2010

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The conversion of dihydroxyl groups to dialdehyde by periodate oxidation is a useful method widely used in derivatization of cellulose. Periodate oxidation is a highly specific reaction to convert 1,2-dihydroxyl groups to paired aldehyde groups without significant side reactions. This reaction cleaves the C2-C3 bond; the resulting compound is the dialdehyde cellulose (DAC). These aldehyde groups of carboxymethyl cellulose interact with amino group of chitosan and their interaction results in the formation Schiff's base with enhanced properties of both the polymers, i.e., of chitosan and carboxymethyl cellulose. Interaction of chitosan and oxidized carboxymethyl cellulose (OCMC) has been carried out with three combinations, i.e., 5, 10, and 15 wt% OCMC with rest of chitosan in 2% lactic acid solution. This new compound is covalently crosslinked which has been analyzed by various techniques like FTIR, TGA, XRD, and SEM.

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Section: Oxidation Of Carboxymethyl Cellulosementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structural characterization of chitosan and oxidized carboxymethyl cellulose based freeze-dried films

2010

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Reaction between glycidylmethacrylate and enzymes and copolymerization of the products obtained

D'Angiuro¹,

Mazzola²,

Cremonesi³

et al. 1978

Angew. Makromol. Chem.

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The reaction between glycidylmethacrylate and proteins (enzymes) leads to products which were separated by gel filtration into different fractions, which were examined by means of various analytical techniques.Spectroscopic analysis gives evidence of a perturbation of the chromophore groups of the protein as a consequence of a strong interaction with the bifunctional monomer. This perturbation is caused by reaction under formation of a covalent bond as verified especially by electrophoretic and amino acid analysis and secondarily by IR and NMR spectra.The competitive reactivity of epoxy ring and double bond of the monomer can be established by the comparative reaction between monofunctional monomers, as methylacrylate and methylmethacrylate, and the protein. The effect of the reaction with glycidylmethacrylate on the stability of the enzymes is a decrease of the specific activity from 5 to 30% for reaction periods ranging from 12 to 120 h.The copolymerization of the reaction products produces materials having activities dependent on the degree of substitution of the fractions, and recoveries of activity higher than SO%,. ZUSAMMENFASSUNG:Die Reaktion von Glycidylmethacrylat mit Proteinen fihrt zu einem Mischprodukt, aus dem durch Gel-Filtration einige Fraktionen isoliert werden konnen. Letztere wurden mit Hilfe verschiedener Methoden untersucht. Die spektroskopische Untersuchung zeigt cine Storung der chromophoren Gruppen des Proteins, die von der Wirkung des bifunktionellen Monomeren verursacht wird. Die anderen Untersuchungsmethoden zeigen, daB cine Reaktion unter Bildung von kovalenten Bindungen eingetreten ist, was erstens durch die elektrophoretische Analyse der Aminosiiuren, und zweitens durch IR-und NMR-Untersuchungen bestatigt wid. Die Reaktionsfahigkeit des Epoxyringes und der Doppelbindung geht aus der Reaktion der monofunktionellen Verbindungen (Methylacrylat, Methylmethacrylat) mit den Proteinen hervor. Die Reaktion von Glycidylmethacrylat bewirkt eine Vermindcrung der spezifischen Aktivitat der Enzyme um 5 30% innerhalb 31 L. D'Angiuro, G. Mauola, P. Cremonesi, and €3. E'ocher einer Reaktionszeit von 12-120 h. Die Copolymerisation dcr Reaktionsproduktc liefert Matcrialien. dercn Aktivitat vom Substitutionsgrad der Fraktionen abhlngig ist. Die Wiedergewinnung der Aktivitat betragt ca. 50%.

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Continuous monitoring in enzyme immobilization

Bovara¹,

Pasta²,

Cremonesi³

1976

Biotech & Bioengineering

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Peroxidase immobilized on acrylic copolymer beads

Abstract: Die Angewandte Makromolekulare Chemie 48 ( 1 975) 17-27 (Nr. 697) Laboratorio di Chimica degli Ormoni, Milano and Stazione Sperimentale per la Cellulosa, Carta e Fibre tessili vegetali ed artificiali, Milano, Italy

Cited by 8 publications

References 7 publications

Structural characterization of chitosan and oxidized carboxymethyl cellulose based freeze-dried films

Structural characterization of chitosan and oxidized carboxymethyl cellulose based freeze-dried films

Reaction between glycidylmethacrylate and enzymes and copolymerization of the products obtained

Continuous monitoring in enzyme immobilization

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