Peroxidase as a Marker for Rooting Improvement of Cynara scolymus L. Cultured in vitro

Anatomical and biochemical changes during root formation in oak and apple shoots cultured in vitro

“…Again, Zhou et al (1992) (Moncousin and Gaspar, 1983;Moncousin et al, 1988;Gaspar, 1989), the peroxidase activity peak marks the end of the "induction" stage of root formation. In Sequoiadendron giganteum the peroxidase peak is reached on d 17, when the meristemoids are formed (Berthon et al, 1987); in oak, the maximum peroxidase activity is reached on d 6, when root primordia have already been formed (apparently a couple of days after the formation of meristemoids).…”

Effects of cutting size and exogenous hormone treatment on rooting of shoot cuttings in Norway spruce [Picea abies (L.) Karst.]

“…Applying IBA may have an indirect influence by enhancing the speed of translocation and movement of carbohydrates to the base of cuttings and consequently stimulate rooting (Davies et al 1990;Aminah et al 1995), so the rooting rate may be enhanced by the IBA treatment (Aminah et al 1995). This may also be related to total phenolic content and peroxidase activity, which are higher in IBA-treated cuttings, particularly during the initiation and expression phases (Moncousin and Gaspar 1983;Rout 2006). Similar to IBA, NAA treatment promotes rooting in conifer stem cuttings (Copes and Mandel 2000;Ragonezi et al 2010), but in the present study we found that NAA may be an inhibitor, which is in accordance with a study on apples (De Klerk et al 1997).…”

Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots

“…Continuous darkness instead of a 16-8 day-night cycle during elongation and root initiative phases of apple shoots favoured successive increase and decrease of peroxidase activity, and resulted in higher percentages of rooted plantlets (Druart et al, 1982). On the contrary, enhancement of peroxidase activity of globe artichoke shoots was favoured by continuous light, whereas a reduced photoperiod or darkness was necessary to achieve the decrease in peroxidase activity and to obtain rooting (Moncousin and Gaspar, 1983 (Gaspar et al, 1977). A simple classification between auxin synergists and antagonists thus no longer holds true (Gaspar, 1989 figure 3 allow the following conclusions to be made (Kevers et al, 1990Kevers and Gaspar, 1992 (Berthon et al, 1992).…”