Peroxidase and chitinase isoenzyme activities during root infection of Chinese cabbage with Plasmodiophora brassicae

Abstract

W. 1994. Peroxidase and chitinase isoenzyme activities during root infection of Chinese cabbage with Plasmodiophora brassicae. -Physioi. Plant. 90: 661-670.Roots of two Chinese cabbage (Brassica campestris L. ssp. pekinensts) varieties, one tolerant and one susceptible, were inoculated with Plasmodiophora brassicae in liquid medium and in soil. Chitina.se and peroxidase activities were determined in roots and shoots 1-21 days after inoculation with resting .spores of Plasmodiophora and the enzyme activities compared with healthy tissue of the same age. In infected roots of the susceptible variety 'Granat" chitinase activity was higher than in the control 10 days after inoculation w ith spores. In the tolerant variety "Parkin' we detected an increase in chitinase activity at the same time, which was about twice that of 'Granat'. Chitinase activity in 'Granat' was also enhanced on day 13, 14 and 17 after inoculation, whereas chitinase activity in 'Parkin' was lower in the infected roots than in the controls during that period. In the shoots no correlation between chitinase activity and infection in the two varieties was observ'ed. Chitinase from Chinese cabbage was further characterized and showed a pH optimum at pH 4.5-5.5 and a temperature optimum at 35^5°C. After isoelectric focusing 7 isoenzymes were discovered, but there were almost no differences between infected and healthy root extracts. Two isoenzymes with pi 8.7 and 8.8 showed cross-reactivity w ith an antiserum against bean chitinases. The molecular mass of these isoenzymes was determined as 33 kDa. Total peroxidase activity was generally higher in root tissue of both varieties than in the shoots. Peroxidase activity was increased most prominently in infected "Granat' roots on day 13 after inoculation and of hoth varieties on day 17 compared to the controls, ln clubbed tis.sue of 'Granat' a specific peroxidase isoenzyme appeared the first time 21 days after inoculation and was most prominent 28-30 days after inoculation. This isoenzyme had a molecular mass of ca 24 kDa and a pi of ca 8.8. With respect to our results the strategy of the Plasmodiophorales for plant attack is discussed.

Purification and properties of chitinase from cabbage stems with roots

“…These results indicated that at least two chitinases exist in different tissue of cabbage. Ludwing-Muller et al (23) found that 7 chitinase isozymes exist in chinese cabbage roots as analyzed by isoelectric focusing. Two isozymes with pI 8.7 and 8.8 showed cross-reactivity with an antiserum against bean chitinases.…”

Effect of temperature on primary infection by Plasmodiophora brassicae and initiation of clubroot symptoms

“…Microscopic examination of RHI has been used to estimate the activity of P. brassicae (Samuel & Garrett, 1945) and spore density (Hwang et al. , 2011) in soil, to assess hormonal activity (Islam, 2009), enzyme activity (Ludwig‐Müller et al. , 1994), and cultivar resistance (Hwang et al.…”

Physcomitrium patens Mutants in Auxin Conjugating GH3 Proteins Show Salt Stress Tolerance but Auxin Homeostasis Is Not Involved in Regulation of Oxidative Stress Factors

“…For total peroxidase activity, significant differences after 250 mM NaCl treatment were found, which persisted over all measured time points ( Figure 9 a). Analysis of specific peroxidase isoforms using a native PAGE method [ 54 ] showed some quantitative differences between the treated WT and mutant plants, being the only detectable differences in the whole dataset ( Figure 9 b). All bands increased in intensity in the salt treated samples of WT and mutants, and an increase in a set of isoenzymes with high molecular mass (marked by asterisks) was found that was higher in the two mutant lines than in the WT.…”