Peroxidase and Catalase in Saliva.,

Nickerson, John F.; Kraus, Frederick W.; Perry, William I.

doi:10.3181/00379727-95-23236

Cited by 34 publications

(11 citation statements)

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“…Westergren, 1979;Tanzer et al, 2001a). Furthermore, previous reports of high peroxidase levels in saliva of rats and humans (Nickerson et al, 1957;Morioka et al, 1969;Ihalin et al, 2006) also suggest the impotence of S. gordonii-produced peroxide to inhibit S. mutans. Recent observations on advanced caries lesions in young human teeth, using bacterial 16S sequence analysis methods, are consistent with the present data, and indicate that S. gordonii diminishes greatly in caries-associated plaque biofilm, while S. mutans persists (Gross et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Streptococcus mutans Out-competes Streptococcus gordonii in vivo

et al. 2012

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Streptococcus gordonii and Streptococcus mutans avidly colonize teeth. S. gordonii glucosyltransferase (GtfG) and amylase-binding proteins (AbpA/AbpB), and S. mutans glucosyltransferase (GtfB), affect their respective oral colonization abilities. We investigated their interrelationships and caries association in a rat model of human caries, examining the sequence of colonization and non- vs. high-sucrose diets, the latter being associated with aggressive decay in humans and rats. Virulence-characterized wild-types of both species and well-defined mutants of S. gordonii with interrupted abpA and gtfG genes were studied. While both S. gordonii and S. mutans were abundant colonizers of rat’s teeth in the presence of either diet, if inoculated singly, S. mutans always out-competed S. gordonii on the teeth, independent of diet, strain of S. mutans, simultaneous or sequential inoculation, or presence/absence of mutations of S. gordonii’s abpA and gtfG genes known to negatively or positively affect its colonization and to interact in vitro with S. mutans GtfB. S. mutans out-competed S. gordonii in in vivo plaque biofilm. Caries induction reflected S. mutans or S. gordonii colonization abundance: the former highly cariogenic, the latter not. S. gordonii does not appear to be a good candidate for replacement therapy. These results are consistent with human data.

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Section: Discussionmentioning

confidence: 99%

Streptococcus mutans Out-competes Streptococcus gordonii in vivo

et al. 2012

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“…Enzymes. Whether carbohydrate-containing or not, numerous enzymes are produced by the gland cells, including amylase, renin, kallikrein, nerve growth factor and other peptidases, peroxidase (Nickerson, Kraus and Perry, 1957;Morrison and Allen, 1963). ribonucleases (Eichel, Conger and Chernick, 1964;Mayner andAckerman.…”

Section: (Ii) Fntrinsieproteinsmentioning

confidence: 99%

Composition of Saliva in Mammalia

Schneyer

1981

Aust J Exp Biol Med

150

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“…One ml. treated saliva was inoculated into [15][16][17][18][19][20] ml. broth and incubated aerobically at 370 C. for 72 hours before results were recorded.…”

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Sterilization and Storage of Saliva

Williams

Kraus

1963

J Dent Res

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and Veterans Administration Hospital, Birmingham, Alabama Studies of nutrient and antibacterial properties of saliva require a saliva that is free of viable microorganisms. In the past, sterile saliva has been produced either by one of the methods used to sterilize bacteriologic media or by aseptic collection from the ducts of the salivary glands. The changes in salivary components caused by sterilization have been mentioned in very few reports. A number of investigators'-8 have characterized growth-inhibitory constituents of saliva as being heat-stable or heat-labile, filterable or non-filterable, and present in sediment or supernatant fluid. However, the emphasis was not on changes brought about in saliva by the process of sterilization. Jackson and Williams9 have sterilized whole saliva by the addition of ethylene oxide (0.6-2.0 per cent incubated with whole saliva for 18-24 hours at 370 C.) and reported that amylolytic activity was reduced by 20 per cent.Separate salivary secretions, which can be collected sterile or with few bacteria, lack some of the constituents of whole saliva. Parotid or submaxillary secretions obtained by aseptic cannulation have been used in studies of antibacterial factors'0 and of substances1' supporting the growth of bacteria. Submaxillary and sublingual secretions collected with the aid of a sterile Schneyer segregator were sterilized by brief exposure to 2537 A ultraviolet light.12' 13 This treatment did not significantly reduce their amylolytic activity.The present report deals with the application of 5 sterilization methods to whole saliva and with the effects of these procedures on some salivary components. The principal goal was reproducible sterility of saliva. In addition, the sterile saliva should have suffered a minimum loss of bacterial metabolites and host materials such as enzymes and plasma proteins. Sterile and unsterile saliva were compared for concentrations of amylase, catalase, and peroxidase. The effect of sterilization upon other proteins in saliva was determined by precipitin tests with specific antisera. Similar tests were also applied in evaluating the effects of various conditions of storage.Materials and Methods SALIVA.-Paraffin-stimulated saliva was collected from employees of a research laboratory and of a dental clinic. Samples were chilled immediately following collection. Food particles, paraffin, mammalian cells, and clumps of bacteria were removed by filtering through gauze or washed glass wool.* The saliva was pooled and stored in Erlenmeyer flasks at 40 C. for 1-2 hours before sterilization.

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Peroxidase and Catalase in Saliva.,

Cited by 34 publications

References 0 publications

Streptococcus mutans Out-competes Streptococcus gordonii in vivo

Streptococcus mutans Out-competes Streptococcus gordonii in vivo

Composition of Saliva in Mammalia

Sterilization and Storage of Saliva

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