Peroxidase and catalase activity in leaves of Halimione portulacoides exposed to salinity

Abstract

1984. Peroxidase and catalase activity in leaves of Halimione portuiacoides exposed to salinity. -Physiol. Plant. 62; 238-244.The effect of high NaCl concentrations on the activity of catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7) and malate dehydrogenase (NAD+-linked; EC 1.1.1.37) from leaves of Halimione portuiacoides (L.) Aelien was studied. The plants were exposed to high salinity during growth and enzyme activity was measured either in the absence or in the presence of various concentrations of NaCl. Increasing salinity in vitro induced three types of effects; (1) an increase in activity (peroxidase); (2) a decrease in activity (catalase); (3) stimulation by low salt concentration but inhibition by higher concentrations (malate dehydrogenase). Salinity in vivo induced a marked decrease in catalase and maiate dehydrogenase activities. However, peroxidase in vivo showed an optimum curve of activity vs external NaCl concentration, with an optimum at ca 1 JW NaCI. Exposure of plants to salinity induced changes in the properties of the enzyme proteins; they precipitated at a higher (NH4)2SO4 concentration, were eluted later during Sephadex G-200 filtration, and showed a shift in the maximal, minimal and optimal temperatures. These data are interpreted as evidence for conformational changes in the enzymes due to prolonged exposure to high salinity stress; such changes could be disruption into monomers (catalase and malate dehydrogenase), or changes in molecular shape (in the peroxidase).Additional key words -Malate dehydrogenase.

Response of Rice under Salinity Stress: A Review Update

“…The active role of the anti oxidative system has also been observed in roots of rice cultivars differing in salt tolerance [113][114][115][116]. Sese and Tobita [109] reported a significant decrease of SOD activity in the salt-susceptible varieties whereas an increase in SOD activity in the salt-tolerant variety in response to salt treatment [115]. An increase in peroxidase activity in salt-tolerant cultivars under salt stress has been recorded by various researchers [117][118][119].…”

“…The oxidative damage caused is a measure of poise between the formation of ROS and its subsequent removal by the antioxidative scavenging system [109,112]. The active role of the anti oxidative system has also been observed in roots of rice cultivars differing in salt tolerance [113][114][115][116]. Sese and Tobita [109] reported a significant decrease of SOD activity in the salt-susceptible varieties whereas an increase in SOD activity in the salt-tolerant variety in response to salt treatment [115].…”

Salinity Dependent Photosynthetic Response and Regulation of Some Enzymes in Halophytes from Indian Sundarbans

“…They observed that NaCl increased peroxidase activity which could be related to regulation of membrane permeability, cell wall formation and oxidation of accumulated substances due to salt stress. It was also proved that peroxidases are enzymes related to polymer synthesis in cell wall (Bowles, 1990), as well as with prevention of oxidation of membrane lipids (Kalir et al, 1984). Biosynthesis of Esterase (EST) revealed that in all five species it is in higher amount in the in-situ taxa investigated.…”

Influence of temperature on biomass, iron metabolism and some related bioindicators in tomato and watermelon plants

“…Extraction and assay of GPX activity were carried out as described Kalir et al (1984) and Ruiz et al (1998) ) at 470 nm. Extraction and assay of CAT activity were determined as described Badiani et al (1990) ) at 240 nm for three minutes.…”