Peroxidase Activity and Isoperoxidase Composition in Cultured Stem Tissue, Callus and Cell Suspensions of Actinidia chinensis

Hirsch, Anne-Marie; Fortune, David H.

doi:10.1016/s0044-328x(84)80048-3

Cited by 19 publications

(6 citation statements)

References 14 publications

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“…Gene expression and activity of isoenzymes respond to environmental changes [25,28,37] and the question is whether environmentally induced changes in isoenzyme activity might be persistent. Persistence of the differences between peroxidase isoenzymes of flax genotrophs in tissue culture [14] and callus production of isoperoxidases related to sex differentiation (which is under hormonal control) in the whole plant [22] give a positive answer. The present results similarly confirm that mechanically (through rubbing) induced changes in isoperoxidase pattern in an organized organ, the internode, are transmittable to callus.…”

Section: Discussionmentioning

confidence: 99%

“…Callus anatomical and biochemical characteristics certainly can be affected by the culture media and the environmental conditions since they can be changed by chemical and physical manipulations [8,38]. There are, however, some indications that calli may continue to express biochemical traits of the mother plants [14,22,36,40]. The extent of such a biochemical inheritance has been investigated here using calli originated from internodes (rubbed and non-rubbed) of Bryonia dioica.…”

Section: Introductionmentioning

confidence: 99%

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Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

Bourgeade

Boyer

Jaegher

et al. 1989

Plant Cell Tiss Organ Cult

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Calli have been initiated in vitro from young internodes (control and rubbed) of Bryonia dioica, where previously it had been shown, using intact plants, that rubbing induced limited growth through enhanced lignification. Calli derived from rubbed internodes were somewhat more compact and showed biochemical changes, i.e. enhanced activity of total peroxidase and isoperoxidases, enhanced production of l-aminocyclopropane-l-carboxylic acid (ACC) and ethylene, enhanced tissue capacity to convert ACC into ethylene, enhanced activity of phenylalanine ammonia-lyase (PAL) and higher content of lignin, which characterized rubbed internodes. Differences in ethylene metabolism between the two types of calli tended to fade from the third week onwards of initial culture, whereas lignin content, peroxidase activity and peroxidase isoenzyme pattern appeared to be more persistant rubbinginduced markers for several subcultures. The results point to the persistance of environmentally induced changes in gene expression.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

Bourgeade

Boyer

Jaegher

et al. 1989

Plant Cell Tiss Organ Cult

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“…The release of peroxidase from cultured plant cells into the surrounding culture medium has been reported for several species (MISAWA & MARTIN 1972;GAM-BURG et al 1977;MADER et al 1981; STEPHAN & VAN HUYSTEE 1981;HIRSCH & FORTUNE 1984). The predominant part of the peroxidase in the medium appears to be the result of an active secretion (STICHER et al 1981;KEVERS et al 1982; VAN HUYSTEE & LoBARZEWSKI 1982).…”

Section: Introductionmentioning

confidence: 96%

Release of Peroxidases by Cultured Potato Cells

Bredemeijer¹,

Burg²,

Ramulu³

et al. 1985

Acta Botanica Neerlandica

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Electrophoretic analysis of peroxidase isoenzymes and other proteins present in the culture medium of potato (Solanum tuberosum L. cv. Bintje) cells in suspension culture was carried out by isoelectric focusing and starch gel electrophoresis. The results showed that during the exponential growth phase, the number of peroxidase isoenzymes as well as their activities increased. In addition, several other proteins have increased in the culture medium.The high specific activity of medium peroxidase as compared to that of cellular peroxidase and differences in the release of various peroxidase isoenzymes suggest that the peroxidase was released by specific secretion.

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“…Hirsch & Fortune (1984) showed that male and female kiwifruit plants are characterized by a different isoperoxidase composition as also found in Ricinus cornmunis (Jaiswal & Kumar, 1983), Cannabis sativa (Sriram & Mohan Ram, 1984), Dioscorea composita (Bhargava & Koul, 1985), and Carica papaya (Sriprasertsak et al, 1988).…”

Section: Discussionmentioning

confidence: 92%

Sex modification in Actinidia deliciosa var. deliciosa

1992

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The aim of the trial was to determine whether growth regulators and injuries could affect sex expression in the cultivated kiwifruit. Growth substances (NAA, BAP, GA, ethephon, ABA, TIBA, and CCC) were applied at 1% concentration in lanolin (w/w) to shoots of 17-year old plants of the staminate clone Matua and the pistillate clone Hayward. Injuries consisted in shoot defoliation or removal of a ring of phloematic tissue from the laterals. Both the application of growth regulators and injuries were done about 5 weeks before anthesis. In the pistillate clone, no treatment resulted in overcoming pollen grain sterility; ABA and GA increased the stigma-style number as well as the density of papillae on the stigma surface. A positive correlation was found between stigma-style number and pedicel length (r = 0.660). In the staminate clone, pollen grain viability was significantly altered by treatment; the highest (79.2%) and lowest (10.3%) values were recorded for ABA and GA, respectively. A negative correlation was found between pollen grain viability and pedicel length (r--0.648). As a general rule, treatments which depressed pollen grain viability allowed the development of a variable number of styles. About 20 styles were consistently produced when GA was applied; the gibberellin-caused feminization of kiwifruit was confirmed in a repeated trial. The data collected also suggest that sex expression can be slightly changed by injuries; contrariwise, rather good results can be achieved by using a growth regulator earlier in the season or before flower initiation.

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Peroxidase Activity and Isoperoxidase Composition in Cultured Stem Tissue, Callus and Cell Suspensions of Actinidia chinensis

Cited by 19 publications

References 14 publications

Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

Release of Peroxidases by Cultured Potato Cells

Sex modification in Actinidia deliciosa var. deliciosa

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