Permissivity of Vero cells, human pneumocytes and human endometrial cells to Waddlia chondrophila

Kebbi-Beghdadi, Carole; Cissé, Ousmane; Greub, Gilbert

doi:10.1016/j.micinf.2011.01.020

Cited by 54 publications

(73 citation statements)

References 33 publications

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“…We found that W. chondrophila readily infected HeLa cells (see below) as well as various other human cell lines (data not shown) and formed big inclusions. W. chondrophila growth was visible after about 12 h; at later stages, growth was very rapid and the inclusions appeared irregular, similar to the patterns reported in other human cells (13) or an ovine (15) cell line. On top of the main inclusion formed in an infected HeLa cell, growth of the bacteria involved the appearance of cytosolic "holes" that increased in size over about 1 to 4 h before gaining access to the inclusion and being filled up by the bacteria ( Fig.…”

Section: Resultssupporting

confidence: 84%

“…Close proximity of W. chondrophila inclusions with mitochondria has been shown in bovine, monkey, and mouse cells as well as in human macrophages and Ishikawa epithelial cells (13,22). This close association with mitochondria was also seen in HeLa cells (Fig.…”

Section: Resultssupporting

confidence: 59%

“…chondrophila can grow in human cells (12)(13)(14), and experimental growth was recently also reported in an ovine trophoblast cell line (15). We here infected HeLa human cervical epithelial cells (the standard host cell line for the study of C. trachomatis) with W. chondrophila and tested for the morphology of the vacuole, association with the endolysosomal pathway, cytoskeletal recruitment, inhibition of apoptosis, cleavage of host cell proteins, the ability to compete with C. trachomatis in coinfection experiments, fragmentation of the Golgi apparatus, and transport of sphingomyelin to the Waddlia inclusion.…”

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confidence: 99%

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In Contrast to Chlamydia trachomatis, Waddlia chondrophila Grows in Human Cells without Inhibiting Apoptosis, Fragmenting the Golgi Apparatus, or Diverting Post-Golgi Sphingomyelin Transport

et al. 2015

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bThe Chlamydiales are an order of obligate intracellular bacteria sharing a developmental cycle inside a cytosolic vacuole, with very diverse natural hosts, from amoebae to mammals. The clinically most important species is Chlamydia trachomatis. Many uncertainties remain as to how Chlamydia organizes its intracellular development and replication. The discovery of new Chlamydiales species from other families permits the comparative analysis of cell-biological events and may indicate events that are common to all or peculiar to some species and more or less tightly linked to "chlamydial" development. We used this approach in the infection of human cells with Waddlia chondrophila, a species from the family Waddliaceae whose natural host is uncertain. Compared to C. trachomatis, W. chondrophila had slightly different growth characteristics, including faster cytotoxicity. The embedding in cytoskeletal structures was not as pronounced as for the C. trachomatis inclusion. C. trachomatis infection generates proteolytic activity by the protease Chlamydia protease-like activity factor (CPAF), which degrades host substrates upon extraction; these substrates were not cleaved in the case of W. chondrophila. Unlike Chlamydia, W. chondrophila did not protect against staurosporine-induced apoptosis. C. trachomatis infection causes Golgi apparatus fragmentation and redirects post-Golgi sphingomyelin transport to the inclusion; both were absent from W. chondrophila-infected cells. When host cells were infected with both species, growth of both species was reduced. This study highlights differences between bacterial species that both depend on obligate intracellular replication inside an inclusion. Some features seem principally dispensable for intracellular development of Chlamydiales in vitro but may be linked to host adaptation of Chlamydia and the higher virulence of C. trachomatis.

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Section: Resultssupporting

confidence: 84%

Section: Resultssupporting

confidence: 59%

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confidence: 99%

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In Contrast to Chlamydia trachomatis, Waddlia chondrophila Grows in Human Cells without Inhibiting Apoptosis, Fragmenting the Golgi Apparatus, or Diverting Post-Golgi Sphingomyelin Transport

et al. 2015

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“…Regarding Chlamydiales ecology, these bacteria are extremely widespread around the world, with a huge range of nonhuman hosts, including vertebrates, such as mammals, marsupials, fish, amphibians, birds, and reptiles, as well as invertebrates such as insects, molluscs, crustaceans, and protozoa (14)(15)(16)(17). This peculiar ability of most Chlamydia-related bacteria to grow within protozoa has been used to successfully isolate novel Chlamydiae by using amoebae in an innovative cell culture system called amoebal coculture (18,19).…”

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confidence: 99%

The High Prevalence and Diversity of Chlamydiales DNA within Ixodes ricinus Ticks Suggest a Role for Ticks as Reservoirs and Vectors of Chlamydia-Related Bacteria

Pilloux

Aeby

Gäumann

et al. 2015

Appl Environ Microbiol

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bThe Chlamydiales order is composed of nine families of strictly intracellular bacteria. Among them, Chlamydia trachomatis, C. pneumoniae, and C. psittaci are established human pathogens, whereas Waddlia chondrophila and Parachlamydia acanthamoebae have emerged as new pathogens in humans. However, despite their medical importance, their biodiversity and ecology remain to be studied. Even if arthropods and, particularly, ticks are well known to be vectors of numerous infectious agents such as viruses and bacteria, virtually nothing is known about ticks and chlamydia. This study investigated the prevalence of Chlamydiae in ticks. Specifically, 62,889 Ixodes ricinus ticks, consolidated into 8,534 pools, were sampled in 172 collection sites throughout Switzerland and were investigated using pan-Chlamydiales quantitative PCR (qPCR) for the presence of Chlamydiales DNA. Among the pools, 543 (6.4%) gave positive results and the estimated prevalence in individual ticks was 0.89%. Among those pools with positive results, we obtained 16S rRNA sequences for 359 samples, allowing classification of Chlamydiales DNA at the family level. A high level of biodiversity was observed, since six of the nine families belonging to the Chlamydiales order were detected. Those most common were Parachlamydiaceae (33.1%) and Rhabdochlamydiaceae (29.2%). "Unclassified Chlamydiales" (31.8%) were also often detected. Thanks to the huge amount of Chlamydiales DNA recovered from ticks, this report opens up new perspectives on further work focusing on whole-genome sequencing to increase our knowledge about Chlamydiales biodiversity. This report of an epidemiological study also demonstrates the presence of Chlamydia-related bacteria within Ixodes ricinus ticks and suggests a role for ticks in the transmission of and as a reservoir for these emerging pathogenic Chlamydia-related bacteria.

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“…This was partially demonstrated for some Chlamydia-related bacteria, such as Waddlia chondrophila. W. chondrophila can grow not only in amoebae but also in several cell types such as mammalian epithelial cells, macrophages, and fish cell lines [16][17][18] . The amoebal coculture also appears relevant for detecting intracellular bacteria in clinical samples 19,20 , including stools which are heavily contaminated with different bacterial species …”

Section: Introductionmentioning

confidence: 99%

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

Jacquier

Aeby

Lienard

et al. 2013

JoVE

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Intracellular pathogens such as legionella, mycobacteria and Chlamydia-like organisms are difficult to isolate because they often grow poorly or not at all on selective media that are usually used to cultivate bacteria. For this reason, many of these pathogens were discovered only recently or following important outbreaks. These pathogens are often associated with amoebae, which serve as host-cell and allow the survival and growth of the bacteria. We intend here to provide a demonstration of two techniques that allow isolation and characterization of intracellular pathogens present in clinical or environmental samples: the amoebal coculture and the amoebal enrichment. Amoebal coculture allows recovery of intracellular bacteria by inoculating the investigated sample onto an amoebal lawn that can be infected and lysed by the intracellular bacteria present in the sample. Amoebal enrichment allows recovery of amoebae present in a clinical or environmental sample. This can lead to discovery of new amoebal species but also of new intracellular bacteria growing specifically in these amoebae. Together, these two techniques help to discover new intracellular bacteria able to grow in amoebae. Because of their ability to infect amoebae and resist phagocytosis, these intracellular bacteria might also escape phagocytosis by macrophages and thus, be pathogenic for higher eukaryotes. Video LinkThe video component of this article can be found at

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Permissivity of Vero cells, human pneumocytes and human endometrial cells to Waddlia chondrophila

Cited by 54 publications

References 33 publications

In Contrast to Chlamydia trachomatis, Waddlia chondrophila Grows in Human Cells without Inhibiting Apoptosis, Fragmenting the Golgi Apparatus, or Diverting Post-Golgi Sphingomyelin Transport

In Contrast to Chlamydia trachomatis, Waddlia chondrophila Grows in Human Cells without Inhibiting Apoptosis, Fragmenting the Golgi Apparatus, or Diverting Post-Golgi Sphingomyelin Transport

The High Prevalence and Diversity of Chlamydiales DNA within Ixodes ricinus Ticks Suggest a Role for Ticks as Reservoirs and Vectors of Chlamydia-Related Bacteria

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

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