Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii

Sobotta, Katharina; Bonkowski, Katharina; Liebler-Tenorio, Elisabeth M.; Germon, Pierre; Rainard, Pascal; Hambruch, Nina; Pfarrer, Christiane; Jacobsen, Ilse D.; Menge, Christian

doi:10.1186/s13567-017-0430-9

Cited by 21 publications

(18 citation statements)

References 44 publications

(70 reference statements)

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“…In contrast, Arricau-Bouvery et al [40] detected C. burnetii in caprine faeces after 25 days of subcutaneous infection and just before the goats aborted. In a recently published study, Sobotta et al [41] infected bovine epithelial cells from the intestine with C. burnetii. The intestinal epithelial cells allowed moderate invasion of the pathogen but there was little further propagation of bacterial numbers within these cells.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

et al. 2020

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The main reservoir of Coxiella (C.) burnetii are ruminants. They shed the pathogen through birth products, vaginal mucus, faeces and milk. A direct comparison of C. burnetii excretions between naturally infected sheep and goats was performed on the same farm to investigate species-specific differences. The animals were vaccinated with an inactivated C. burnetii phase I vaccine at the beginning of the study period for public health reasons. Vaginal and rectal swabs along with milk specimens were taken monthly during the lambing period and once again at the next lambing season. To estimate the environmental contamination of the animals’ housings, nasal swabs from every animal were taken simultaneously. Moreover, dust samples from the windowsills and straw beddings were collected. All samples were examined by qPCR targeting the IS1111 gene and the MLVA/VNTR typing method was performed. Whole genome sequencing was applied to determine the number of IS1111 copies followed by a calculation of C. burnetii genome equivalents of each sample. The cattle-associated genotype C7 was detected containing 29 IS1111 copies. Overall, goats seem to shed more C. burnetii through vaginal mucus and in particular shed more and for longer via the rectal route than sheep. This is supported by the larger quantities of C. burnetii DNA detected in caprine nasal swabs and environmental samples compared to the ovine ones. Transmission of C. burnetii from cattle to small ruminants must also be considered.

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Section: Discussionmentioning

confidence: 99%

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

et al. 2020

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“…The effective control of these and other pathogens in cattle has significant economic implications, and is important for food security as many also have zoonotic potential. Few physiologically-relevant in vitro systems are available to accurately study the interactions between enteric pathogens and the bovine intestinal epithelium, and most data have been derived from the analysis of 2D monolayers of epithelial cell lines or primary epithelial cells [ 27 , 28 ]. In the current study we describe the development of a 3D in vitro culture system that is more representative of the cellular diversity within the bovine intestinal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Development of in vitro enteroids derived from bovine small intestinal crypts

Hamilton

Young

Jayaraman

et al. 2018

Vet Res

116

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Cattle are an economically important domestic animal species. In vitro 2D cultures of intestinal epithelial cells or epithelial cell lines have been widely used to study cell function and host–pathogen interactions in the bovine intestine. However, these cultures lack the cellular diversity encountered in the intestinal epithelium, and the physiological relevance of monocultures of transformed cell lines is uncertain. Little is also known of the factors that influence cell differentiation and homeostasis in the bovine intestinal epithelium, and few cell-specific markers that can distinguish the different intestinal epithelial cell lineages have been reported. Here we describe a simple and reliable procedure to establish in vitro 3D enteroid, or “mini gut”, cultures from bovine small intestinal (ileal) crypts. These enteroids contained a continuous central lumen lined with a single layer of polarized enterocytes, bound by tight junctions with abundant microvilli on their apical surfaces. Histological and transcriptional analyses suggested that the enteroids comprised a mixed population of intestinal epithelial cell lineages including intestinal stem cells, enterocytes, Paneth cells, goblet cells and enteroendocrine cells. We show that bovine enteroids can be successfully maintained long-term through multiple serial passages without observable changes to their growth characteristics, morphology or transcriptome. Furthermore, the bovine enteroids can be cryopreserved and viable cultures recovered from frozen stocks. Our data suggest that these 3D bovine enteroid cultures represent a novel, physiologically-relevant and tractable in vitro system in which epithelial cell differentiation and function, and host–pathogen interactions in the bovine small intestine can be studied.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0547-5) contains supplementary material, which is available to authorized users.

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“…Mice and guinea pigs are also obligate nasal breathers and as such possess a greater surface area in their upper respiratory tract as well as a much higher percentage of olfactory epithelium than humans (27,28). This can lead to much higher rates of nasopharyngeal colonization following intranasal inoculation, prior to reaching the lower pulmonary tract, and potential infection of epithelial cells via nasal-associated lymphoid tissue, resulting in extra pulmonary complications (29,30). Finally, the inhaled dose received by animals under whole-body or nose/face delivery methods is often dependent on the estimated respiratory rate of the recipient animals, which can vary and lead to inaccuracy and variability of the received dose (31).…”

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Coxiella burnetii Intratracheal Aerosol Infection Model in Mice, Guinea Pigs, and Nonhuman Primates

et al. 2019

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Coxiella burnetii, the etiological agent of Q fever, is a Gram-negative bacterium transmitted to humans by inhalation of contaminated aerosols. Acute Q fever is often self-limiting, presenting as a febrile illness that can result in atypical pneumonia. In some cases, Q fever becomes chronic, leading to endocarditis that can be life threatening. The formalin-inactivated whole-cell vaccine (WCV) confers long-term protection but has significant side effects when administered to presensitized individuals. Designing new vaccines against C. burnetii remains a challenge and requires the use of clinically relevant modes of transmission in appropriate animal models. We have developed a safe and reproducible C. burnetii aerosol challenge in three different animal models to evaluate the effects of pulmonary acquired infection. Using a MicroSprayer aerosolizer, BL/6 mice and Hartley guinea pigs were infected intratracheally with C. burnetii Nine Mile phase I (NMI) and demonstrated susceptibility as determined by measuring bacterial growth in the lungs and subsequent dissemination to the spleen. Histological analysis of lung tissue showed significant pathology associated with disease, which was more severe in guinea pigs. Infection using large-particle aerosol (LPA) delivery was further confirmed in nonhuman primates, which developed fever and pneumonia. We also demonstrate that vaccinating mice and guinea pigs with WCV prior to LPA challenge is capable of eliciting protective immunity that significantly reduces splenomegaly and the bacterial burden in spleen and lung tissues. These data suggest that these models can have appreciable value in using the LPA delivery system to study pulmonary Q fever pathogenesis as well as designing vaccine countermeasures to C. burnetii aerosol transmission.

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Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii

Cited by 21 publications

References 44 publications

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

Comparison of Coxiella burnetii Excretion between Sheep and Goats Naturally Infected with One Cattle-Associated Genotype

Development of in vitro enteroids derived from bovine small intestinal crypts

Coxiella burnetii Intratracheal Aerosol Infection Model in Mice, Guinea Pigs, and Nonhuman Primates

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