Permethrin Resistance Mechanisms in the Beet Armyworm (Spodoptera exigua (Huebner))

Shimada, Kei; Natsuhara, Katsuya; Oomori, Yasuhiro; Miyata, Tadashi

doi:10.1584/jpestics.30.214

Cited by 14 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Abamectin, lambda‐cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb have been extensively applied to control S. exigua in China, and very high resistance has been reported in field populations from Guangdong province in South China (Su & Sun, ; Tian et al ., ). The enhanced breakdown of insecticides in resistance populations partially mediated these resistances, and increased P450 activity was the primary cause for metabolic resistance in this pest (Shimada et al ., ; Tian et al ., ; Wang et al ., , ). However, the molecular mechanism for multiple resistances of S. exigua to different insecticides remains to be illuminated, the genes for insecticide detoxification in this pest are also less well reported.…”

Section: Introductionmentioning

confidence: 99%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Zhang

Ren

et al. 2017

Insect Science

View full text Add to dashboard Cite

Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can increase the expression of detoxification enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells; eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression; more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In conclusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification.

show abstract

Section: Introductionmentioning

confidence: 99%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Zhang

Ren

et al. 2017

Insect Science

View full text Add to dashboard Cite

show abstract

“…S. exigua damaged more than 30,000 km 2 only in Henan and Shandong provinces in 1999 (Luo et al 2000) and their management is confounded by their migratory dispersal and rapidly evolving resistance to conventional pesticides (Adamczyk et al 2003;Shimada et al 2005;Wu et al 1995;Wang et al 2001).The increasing resistance in the insects can be due to the increasing metabolic capability of detoxiWcation systems and/or reducing xenobiotic target site sensitivity (Li et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Biological impact of harmaline, ricinine and their combined effects with Bacillus thuringiensis on Spodoptera exigua (Lepidoptera: Noctuidae)

Rizwan-ul-Haq

Mei

et al. 2009

J Pest Sci

View full text Add to dashboard Cite

The history of botanical pesticides reveals that their study did mainly focus on the determination of acute median lethal dose or concentration. In the current situation, it is the dire need to understand the sublethal eVects of the botanical extracts along with the traditional studies of lethal concentrations in order to comprehensively investigate the future role of the botanical extracts as pesticides. This study reveals the eVects of traditionally used medicinal plant extracts harmaline (H) and ricinine (R) either individually or in combination with Bacillus thuringiensis (Bt) on the acute toxicity and sublethal eVects on the nutrition and enzyme system of Spodoptera exigua. Harmaline and ricinine caused reduction in the growth of neonate larvae up to 93.12 and 84.31%. The EC 50 values of harmaline against fourth and Wfth instars were 0.24 and 0.27 mg/ml, but these values remained 0.49 and 0.54 mg/ml against fourth and Wfth instars after being treated with ricinine. The combination of harmaline and ricinine with Bt resulted in the increased eYciency of these chemicals as the mortality percentages signiWcantly increased up to 96 and 87.82% in signiWcantly less exposure time in case of H + Bt and R + Bt respectively, as compared to individual treatments. The nutritional analysis revealed the increased toxicity of harmaline and ricinine in combination with Bt, but H + Bt2 showed the higher eYciency with minimal relative consumption rate 2.50 mg/mg/day, relative growth rate 1.16 mg/mg/day and eYciency of conversion of ingested food 29.66% of control, respectively. Changes in antioxidant enzymes such as superoxide dismutase (SOD) and catalases (CAT) were noticed to some extent over diVerent exposure times at all the treatments. The highest SOD (+37.29%) and CAT (+29.27%) activity was observed at the 6th day of treatment with H + R + Bt2. The study clearly shows the signiWcantly increased eYciency of harmaline and ricinine in combination with Bt against S. exigua. This phenomenon can be helpful in order to develop better control strategies against diVerent notorious pests.

show abstract

“…S. exigua damaged more than 30,000 km 2 only in Henan and Shandong provinces during 1999 (Luo et al, 2000). This insect is not only the major agricultural pest, particularly in the southern USA and Asia, but its management is confounded by its migratory dispersal and rapidly evolving resistance to conventional pesticides (Adamczyk et al, 2003;Shimada et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Study of destruxin B and tea saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

Rizwan-ul-Haq¹,

Hu²,

Mei³

et al. 2009

Appl. Entomol. Zool.

View full text Add to dashboard Cite

This study reveals the individual and synergistic effects of destruxin B (DB) (mycotoxin from Metarhizium anisoplae), tea saponin (Ts) and Bacillus thuringiensis (Bt) against Spodoptera exigua. DB, Ts and Bt reduced the growth of neonate larvae by up to 91.30, 89.17 and 77.17%. EC 50 values of DB against 4th and 5th instars were 0.17 and 0.22 mg ml Ϫ1, 0.35 and 0.41 mg ml Ϫ1 against 4th and 5th instars for Ts and 0.0031 and 0.0035 mg ml Ϫ1 after being treated with Bt. The synergism of DB and Ts with Bt resulted in the increased efficiency of these chemicals as mortality percentages significantly increased up to 94.2% with DBϩTsϩBt followed by DBϩBt with 91.99% and were significantly higher than individual treatments of Bt 65.81% and Ts 76.66%. Nutritional analysis revealed the increased toxicity of DB and Ts in combination with Bt, but DBϩBt showed higher efficiency with minimal relative consumption rate (RCR) 2.69 mg/mg/day, relative growth rate (RGR) 1.33 mg/mg/day and efficiency of conversion of ingested food (ECI) 35.77%, respectively. Changes in antioxidant enzymes, superoxidase dismutase (SOD) and catalases (CAT), were noticed to some extent over different exposure times in all treatments. The highest SOD activity was observed in individual DB treatment, while individual Bt treatment caused the highest variation in CAT values. In all treatments, SOD and CAT values fell sharply on the final day except for individual Bt treatment, with which the values were relatively stable on the final day.

show abstract

Permethrin Resistance Mechanisms in the Beet Armyworm (Spodoptera exigua (Huebner))

Cited by 14 publications

References 33 publications

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Biological impact of harmaline, ricinine and their combined effects with Bacillus thuringiensis on Spodoptera exigua (Lepidoptera: Noctuidae)

Study of destruxin B and tea saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

Contact Info

Product

Resources

About