Permethrin inhibits tube formation and viability of endothelial cells

Lee, Hee-Seop; Song, Hee‐Jung; Park, Yeonhwa; Smolensky, Dmitriy; Lee, Seong‐Ho

doi:10.1002/jsfa.11757

Cited by 1 publication

(1 citation statement)

References 36 publications

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“…Rotenone exposure also inhibited the migration of neural stem cells derived from the rat E16 mesencephalon in a dose-dependent manner [53]. The migration and tube formation capabilities of human umbilical vein endothelial cells were severely repressed in a dose-dependent manner when exposed to permethrin-a synthetic pyrethroid insecticide, facilitated possibly by elevated reactive oxygen species production [54]. Taken together, the biomechanical changes induced by exposure to these pesticide compounds might have contributed to the compromise in cell migration patterns in vitro.…”

Section: Cell Migrationmentioning

confidence: 99%

Phenotypic and Biomechanical Characteristics of Human Fetal Neural Progenitor Cells Exposed to Pesticide Compounds

Sarsfield,

Vasu,

Abuoun

et al. 2023

Biophysica

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Various forms of pesticides have been reported to be among the environmental toxicants, which are detrimental to human health. The active ingredients of these formulations can enter the human body through air, food, or water. Epidemiological studies suggest that these compounds strongly affect the developing brain in fetal and infant stages due to their ability to breach the underdeveloped blood–brain barrier. Since neural progenitor stem cells (NPCs) in the developing brain are the most vulnerable to these compounds, the mechanisms by which NPCs experience toxicity upon exposure to these chemicals must be investigated. Here, we assessed the viability of human fetal NPCs in 2D cultures in the presence of the active ingredients of six widely used pesticides using Live/Dead® and Hoechst staining. The IC50 values ranged from 4.1–201 μM. A significant drop in cell viability with increasing toxicant concentration (p < 0.01) was noted, with the order of toxicity being malathion < 4-aminopyridine < methoprene < prallethrin < temephos < pyriproxyfen. Changes in cellular biomechanical characteristics (Young’s modulus, tether force, membrane tension, and tether radius) were quantified using atomic force microscopy, whereas cell migration was elucidated over 48 h using a customized wound-healing assay. The Young’s modulus of fetal NPCs exposed to IC50/2 doses of these compounds was reduced by 38–70% and that of those exposed to IC50 doses was reduced by 71–80% (p < 0.001 vs. controls for both; p < 0.01 for IC50 vs. IC50/2 for each compound). Similar patterns were noted for tether forces and membrane tension in fetal NPCs. NPC migration was found to be compound type- and dose-dependent. These results attest to the significant detrimental effects of these compounds on various aspects of the human fetal NPC phenotype, and the utility of cell mechanics as a marker to assess developmental neurotoxicity.

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Section: Cell Migrationmentioning

confidence: 99%