Permeation measurement of gestodene for some biodegradable materials using Franz diffusion cells

Abstract

Biodegradable poly(d,l-lactide) (PDLLA), Poly(trimethylene carbonate) (PTMC), polycaprolactone (PCL), poly(caprolactone-co-d,l-lactide) (PCDLLA) and poly(trimethylene carbonate-co-caprolactone) (PTCL) are recently used for clinical drug delivery system such as subcutaneous contraceptive implant capsule due to their biodegradable properties that they could possess long-term stable performance in vivo without removal, however their permeation rate is unknown. In the work, biodegradable material membranes were prepared by solvent evaporation using chloroform, and commercial silicone rubber membrane served as a control. Gestodene was used as a model drug. Gestodene has high biologic progestational activity which allows for high contraceptive reliability at very low-dose levels. The permeation rate of gestodene for several biodegradable materials was evaluated. In vitro diffusion studies were done using Franz diffusion cells with a diffusion area of 1.33 cm(2). Phosphate buffer solution (PBS, pH 7.4), 10% methanol solution and distilled water were taken in donor and receiver chambers at temperature of 37 °C respectively. The in vitro experiments were conducted over a period of 24 h during which samples were collected at regular intervals. The withdrawn samples were appropriately diluted and measured on UV-vis spectrophotometer at 247 nm. Conclusion data from our study showed that permeation rate of PCDLLA with CL ratio more than 70% could be more excellent than commercial silicone rubber membrane. They may be suitable as a candidate carrier for gestodene subcutaneous contraceptive implants in contraceptive fields.

Hydrogels as Drug Delivery Systems: A Review of Current Characterization and Evaluation Techniques

“…As such, it has been the gold standard method to study the topical drug permeation of skin [153][154][155]. The technique could also be applied to synthetic membranes and hydrogel drug delivery systems that are two-dimensional like membranes for transdermal drug administration [156,157]. The device is composed of an upper donor chamber where the drug-loaded hydrogel is placed and a lower acceptor chamber filled with a solution that is agitated via a magnetic stirrer and comporting a sampling port.…”

Challenges and Opportunities in the Oral Delivery of Recombinant Biologics

“…Franz diffusion cells, one of the widely used model to study skin permeation for transdermal drug administration [ 186 , 187 ], is also an approach considered more and more as an intestinal permeability tool. Indeed, similarly to the Ussing chamber system, Franz diffusion cells have two temperature-controlled compartments separated by a fresh human or animal intestinal segment (usually without the underlying serosa and muscle layers): the donor compartment with agitation performed by gas bubbling, and the receptor chamber where a magnetic stirrer adequately homogenizes the solution [ 188 , 189 ] ( Figure 10 ). Contrary to the Ussing chambers having an equal volume in their two compartments, Franz diffusion cells have donor chambers filled with a smaller volume than the receptor ones.…”

“…However, sampling can also be easily performed at different time intervals during the experiments. ers): the donor compartment with agitation performed by gas bubbling, and the receptor chamber where a magnetic stirrer adequately homogenizes the solution [188,189] (Figure 10). Contrary to the Ussing chambers having an equal volume in their two compartments, Franz diffusion cells have donor chambers filled with a smaller volume than the receptor ones.…”

Silica Dioxide Nanofiber Based Drug Delivery System With Sustained Release

“…Franz diffusion cell based experiments are emerging as a generally accepted in vitro release testing method for semisolid formulations [14]. These open chambre based studies can simulate a topical application of the tested formulation by the mean of synthetic membrane, a tissue construct or a biological construct placed between the sample and the acceptor medium.…”