Permeation kinetics of dimethyl sulfoxide in porcine corneoscleral discs

Diffusion kinetics and perfusion times in tissue models obtained by bioorthogonal Raman μ-spectroscopy

“…Thus, DMSO diffusion may be accelerated in the necrotic core of spheroids with diameters >500 μ m, and the average diffusion coefficient is increased. The diffusion coefficients of DMSO in NSC spheroids herein are on the same order of magnitude as values reported for DMSO in macroscopic tissues determined by osmolarity measurement (53 μ m 2 s −1 in porcine corneoscleral discs at 0°C, ( 38 )) or attenuated total reflection Fourier transform infrared analyses (302 μ m 2 s −1 in porcine heart valve tissue at 22°C, ( 13 )).…”

“…Accordingly, we introduced bioorthogonal Raman microspectroscopy to determine the perfusion times of CPA into 3D cell aggregates. Knowledge of perfusion times is the key to appropriate compound application and tissue cryopreservation ( 12 , 38 ). In particular, we investigated the penetration of deuterated dimethyl sulfoxide (DMSO-d6) as the most common and potent permeable CPA.…”