Permeable packings and perfusion chromatography in protein separation

Performance and characterization of a nanophased porous hydroxyapatite for protein chromatography

“…A value of 4.7 Â 10 À9 cm 2 was observed for nanophased BAB 25 Am, 6.2 Â 10 À9 cm 2 for nanophased BAB 30 Am, 5.7 Â 10 À9 cm 2 for Bio-Rad Type I, and 3.7 Â 10 À9 cm 2 for Bio-Rad Type II. Rodrigues (1997) reported for a column packed with 20 Am beads (POROUS 20) a permeability of 2.35 Â 10 À9 cm 2 , whereas for a monolithic silica column that is completely perfused, a permeability of 1.7 Â 10 À9 cm 2 was reported (Ishizuka et al, 2002). For chromatography beads made from porous glass with a particle diameter of 75-125 Am and a pore size of 100 nm, a permeability of 7.1 Â 10 À10 cm 2 was reported (McCue et al, 2003).…”

Metathesis-Based Monolithic Supports: Synthesis, Functionalization and Applications

“…For this particular review, the term monolith or rigid rod shall comprise crosslinked, both inorganic and organic media, which are characterized by a well-defined porosity and are used as supports for interactions/reactions between a solid and a liquid phase. Besides the advantages, such as lower backpressure and enhanced diffusional mass transfer, [30,31] the ease of fabrication and the many possibilities in structural alteration need to be mentioned. Important enough, the enhanced diffusional mass transfer allows separation scientists to run separations at comparably high flow rates of up to 10 mm/s, resulting in fast and highly efficient separations.…”

High‐Performance Liquid Chromatography of Biological Macromolecules

“…Advanced protein separation techniques was the topic of a recent review issue of Journal of Chromatography, including overviews of new packing materials, 96 nonporous sorbents, 97 permeable packings, 98 and protein interactions at sorbent interfaces. 757 Strategies were reviewed for separation of recombinant proteins, 758 sensitive enzymes, 86 modified hemoglobins, 759 lipoproteins, 611 glycoproteins, 760 glycated and carbonylated proteins, 319 and the isolation of allergens.…”

“…Another beneficial effect of the more efficient mass transport in the convective regime is that, unlike conventional porous sorbents, the useful dynamic capacity of the sorbent is preserved at high flow rates. The theory of convection-enhanced internal mass transport in particles has been worked out by Rodrigues et al, 98 adequately describes the mass transfer enhancement for biomacromolecules in perfusive sorbents ( Figure 75). Perfusive sorbents are typically used as relatively large particles (10 -40 µm) in small to medium scale preparative separations.…”