Permeabilization of transformed cells in culture by external ATP

Heppel, Leon A.; Weisman, Gary A.; Friedberg, Ilan

doi:10.1007/bf01870597

Cited by 87 publications

(49 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some cell types [e.g., hepatocytes (63), mast cells (72), polymorphonuclear leukocytes (26), and some transformed cells (149,195,376)], extracellular ATP induces cation flux and increases the permeability of the plasma membrane via the ATP 4Ϫ receptor. The ATP…”

Section: F Atp-induced Permeabilizationmentioning

confidence: 99%

See 1 more Smart Citation

Measurement of Intracellular Calcium

Takahashi

Camacho

Lechleiter

et al. 1999

Physiological Reviews

669

511

View full text Add to dashboard Cite

To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and more recently multiphoton microscopy have allowed the precise spatial analysis of intracellular ion activity at the subcellular level in addition to measurement of its concentration. It is well known that Ca2+ regulates numerous physiological cellular phenomena as a second messenger as well as triggering pathological events such as cell injury and death. A number of methods have been developed to measure intracellular Ca2+. In this review, we summarize the advantages and pitfalls of a variety of Ca2+ indicators used in both optical and nonoptical techniques employed for measuring intracellular Ca2+ concentration.

show abstract

Section: F Atp-induced Permeabilizationmentioning

confidence: 99%

“…One limit of this method is the cell specificity of ATP-induced permeabilization. For example, although some transformed cell lines (e.g., Chinese hamster ovary cells, mouse fibroblasts, mouse melanoma cells, rat kidney cells) exhibit this ATP sensitivity, their nontransformed counterparts do not (149,195).…”

Section: ϫmentioning

confidence: 99%

Measurement of Intracellular Calcium

Takahashi

Camacho

Lechleiter

et al. 1999

Physiological Reviews

669

511

View full text Add to dashboard Cite

show abstract

“…The activity of ecto-ATPases, which hydrolyze ATP (Knowles, 1988;Lin and Russell, 1988), and ectokinases, which use ATP to phosphorylate the outer plasma membrane (Ehrlich et al, 1986), may also play a role in some of these diverse effects of ATP. In addition to these specific effects, extracellular ATP can permeabilize some types of cells, including mast cells (Tatham et al, 1988) and transformed cells in culture (Heppel et al, 1985). ATP is stored and cosecreted with classical neurotransmitters, and may itself be a neurotransmitter (Fredholm and Hedqvist, 1980;Stone, 1981;Gordon, 1986), providing a physiological rationale for the effects of ATP.…”

Section: Introductionmentioning

confidence: 99%

Effects of extracellular ATP on ion transport systems and [Ca2+]i in rat parotid acinar cells. Comparison with the muscarinic agonist carbachol.

Soltoff

McMillian

Cragoe

et al. 1990

The Journal of General Physiology

View full text Add to dashboard Cite

The effects of extracellular ATP on ion fluxes and the intracellular free Ca 2 § concentration ([Ca~+]i) were examined using a suspension of rat parotid acinar cells and were contrasted with the effects of the muscarinic agonist carbachol. Although ATP and carbachol both rapidly increased [Ca2+] i about threefold above the resting level (200-250 nM), the effect of ATP was due primarily to an influx of Ca ~+ across the plasma membrane, while the initial response to carbachol was due to a release of Ca ~ § from intracellular stores. Within 10 s, ATP (1 mM) and carbachol (20 #M) reduced the cellular C1-content by 39-50% and cell volume by 15-25%. Both stimuli reduced the cytosolic K + content by 57-65%, but there were marked differences in the rate and pattern of net K § movement as well as the effects of K + channel inhibitors on the effluxes initiated by the two stimuli. The maximum rate of the ATP-stimulated K § efflux (~2,200 nmol K+/mg protein per min) was about two-thirds that of the carbachol-initiated efflux rate, and was reduced by ~30% (vs. 60% for the carbachol-stimulated K + efflux) by TEA (tetraethylammonium), an inhibitor of the large conductance (BK) K § channel. Charybdotoxin, another K § channel blocker, was markedly more effective than TEA on the effects of both agonists, and reduced the rate of K § effiux initiated by both ATP and carbachol by ~80%. The removal of extracellular Ca ~ § reduced the ATPand the carbachol-stimulated rates of K + efflux by 55 and 17%, respectively. The rate of K + effiux initiated by either agonist was reduced by 78-95% in cells that were loaded with BAPTA to slow the elevation of [Ca2+]i. These results indicated that ATP and carbachol stimulated the effiux of K + through multiple types of K § permeable channels, and demonstrated that the relative proportion of efflux 320THE JOURNAL OF GENERAL PHYSIOLOGY 9 VOLUME 95 9 1990 through the different pathways was different for the two stimuli. ATP and carbachol also stimulated the rapid entry of Na § into the parotid cell, and elevated the intracellular Na § content to 4.4 and 2.6 times the normal level, respectively. The rate of Na § entry through Na+-K+-2CI-cotransport and Na+-H § exchange was similar whether stimulated by ATP, carbachol, or ionomycin, and uptake through these two carrier-mediated transporters accounted for 50% of the ATP-promoted Na § influx. The remainder may be due to a nonselective cation channel and an ATP-gated cation channel that is also permeable to Ca ~ § The consumption of extracellular ATP by an ecto-ATPase (apparent K0.5 for ATP is 0.93 mM) on the plasma membrane limited the duration of the response to ATP when large concentrations of cells were used. The effects of ATP on [Ca~+]i and ion fluxes were blocked specifically by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid), and were more potent in the absence of Mg ~+, suggesting that the active nucleotide moiety was ATP 4-. These studies suggest that ATP may function as a neurotransmitter and modulate fluid secretion by stimula...

show abstract

“…The phenomenon of ATP-induced cell membrane permeabilization has been shown, and partly characterized, in a variety of cell types [1,2,8,14,20]. Although it is generally accepted that the pore-forming P2z receptor, for which ATP 4-serves as a ligand, plays a major role in the permeabilization t~,rocess, the mechanisms underlying this process are only i,artly understood [3,12,[21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts

Arav

Friedberg

1996

FEBS Letters

Self Cite

View full text Add to dashboard Cite

Extracellular ATP (0.6 mM) induces a marked decrease in the membrane potential, followed by an increase in cell membrane permeability in transformed mouse fibroblasts. The effects of the ATP analogs, pICHaIppA and p[NH]ppA (0.6 m VI), on the membrane potential and permeability are much less pronounced. ATP at 0.05 mM has no effect by itself, but markedly increases the analog-induced membrane potential di~ipation and permeability. The data suggest that ATP-induced membrane permeation is composed of two processes: One is common to ATP and its analogs and appears to be a receptormediated process. The second is unique for ATP, effective even at low concentration (0.05 mM), and might be mediated by cell surface enzymes, for which ATP, but not its analogs, serves as a substrate.

show abstract

Permeabilization of transformed cells in culture by external ATP

Cited by 87 publications

References 86 publications

Measurement of Intracellular Calcium

Measurement of Intracellular Calcium

Effects of extracellular ATP on ion transport systems and [Ca2+]i in rat parotid acinar cells. Comparison with the muscarinic agonist carbachol.

Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts

Contact Info

Product

Resources

About