Permeability of lipid bilayers to water and ionic solutes

Deamer, David W.; Bramhall, John

doi:10.1016/0009-3084(86)90069-1

Cited by 316 publications

(231 citation statements)

References 62 publications

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“…Cholesterol could be very important in this respect because its influence on the degree of water penetration [41] can reduce the heterogeneity along the membrane normal. In cholesterol-depleted erythrocyte membranes at 40 ° C, the width of the distribution was larger than that of both the erythrocyte membrane and the liposomes from extracted phospholipids.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fiorini

Valentino

Gläser

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane The fluorescence decay of 1,6 diphenyl-l,3,5-hexatriene (DPH) has been used to characterize aspects of the erythrocyte membrane structure related to the microheterogeneity of the lipid bilayer. The DPH decay has been studied using frequency domain fluorometry and the data analyzed either by a model of discrete exponential components or a model that assumes a continuous distribution of lifetime values. The main intensity fraction was associated with a lifetime value centered at about 11 ns in the erythrocyte membrane, but a short component of very low fractional intensity had to be considered to obtain a good fit to the data. The lifetime value of the long component was insensitive to temperature, while the width of the distribution decreased with increasing temperature. In multilamellar liposomes prepared from phospholipids extracted from the erythrocytes, the long lifetime component showed a temperature dependence. The depletion of 27% of the cholesterol in the erythocyte membrane induced a broadening of the distribution, suggesting a homogenizing effect of cholesterol. This effect has also been detected in egg phosphatidylcholine at a very low cholesterol/phospholipid molar ratio. The role of cholesterol on membrane heterogeneity is discussed in relation to the effect of cholesterol on water penetration.

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Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fiorini

Valentino

Gläser

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…In contrast, with 10 −3 −10 −4 cm s −1 , the P M of protons is about ten orders of magnitude larger. [9][10][11][12][13] In addition to weak acid/base shuttles (e.g. free fatty acids), transient membrane-spanning water wires have also been suggested to be responsible for the inconsistencies between theoretical and measured data.…”

Section: Protons As An Exception To the Meyer-overton Rulementioning

confidence: 99%

“…The average distance of diffusion after 1.5 s is equal to [Eq. (11)]: (11) Thus, within a 1.5 s time span, an acid molecule crosses the distance between the donating electrode and the membrane, the membrane itself and about 1/20 of the receiving compartment. In case of two or three-dimensional diffusion, the distance travelled normal to the membrane would be even smaller.…”

Section: Transport Of Short-chain Carboxylic Acids Through Membranesmentioning

confidence: 99%

110 Years of the Meyer–Overton Rule: Predicting Membrane Permeability of Gases and Other Small Compounds

2009

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The transport of gaseous compounds across biological membranes is essential in all forms of life. Although it was generally accepted that gases freely penetrate the lipid matrix of biological membranes, a number of studies challenged this doctrine as they found biological membranes to have extremely low gas-permeability values. These observations led to the identification of several membrane-embedded "gas" channels, which facilitate the transport of biological active gases, such as carbon dioxide, nitric oxide, and ammonia. However, some of these findings are in contrast to the well-established solubility-diffusion model (also known as the Meyer-Overton rule), which predicts membrane permeabilities from the molecule's oil-water partition coefficient. Herein, we discuss recently reported violations of the Meyer-Overton rule for small molecules, including carboxylic acids and gases, and show that Meyer and Overton continue to rule.

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“…Unfortunately, in silico predictions of the octanol water partition coefficient are not very reliable for small molecules (compare Table 1). Moreover, the structural similarity between H 2 S and H 2 O suggests that caution is required when applying the solubility model to predict P M (14), meaning that even when the partition coefficient is known, the actual and predicted P M may not match each other. The similarity between H 2 S and H 2 O also suggests that H 2 S transport may be facilitated by water channels (15).…”

mentioning

confidence: 99%

No facilitator required for membrane transport of hydrogen sulfide

Mathai

Missner²,

Kügler

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

323

226

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Hydrogen sulfide (H2S) has emerged as a new and important member in the group of gaseous signaling molecules. However, the molecular transport mechanism has not yet been identified. Because of structural similarities with H 2O, it was hypothesized that aquaporins may facilitate H 2S transport across cell membranes. We tested this hypothesis by reconstituting the archeal aquaporin AfAQP from sulfide reducing bacteria Archaeoglobus fulgidus into planar membranes and by monitoring the resulting facilitation of osmotic water flow and H2S flux. To measure H2O and H2S fluxes, respectively, sodium ion dilution and buffer acidification by proton release (H 2S % H ؉ ؉ HS ؊ ) were recorded in the immediate membrane vicinity. Both sodium ion concentration and pH were measured by scanning ion-selective microelectrodes. A lower limit of lipid bilayer permeability to H 2S, P M,H 2 S > 0.5 ؎ 0.4 cm/s was calculated by numerically solving the complete system of differential reaction diffusion equations and fitting the theoretical pH distribution to experimental pH profiles. Even though reconstitution of AfAQP significantly increased water permeability through planar lipid bilayers, PM,H 2 S remained unchanged. These results indicate that lipid membranes may well act as a barrier to water transport although they do not oppose a significant resistance to H 2S diffusion. The fact that cholesterol and sphingomyelin reconstitution did not turn these membranes into an H2S barrier indicates that H 2S transport through epithelial barriers, endothelial barriers, and membrane rafts also occurs by simple diffusion and does not require facilitation by membrane channels. aquaporins ͉ gas transport ͉ membrane permeability ͉ unstirred layer ͉ signaling

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Permeability of lipid bilayers to water and ionic solutes

Cited by 316 publications

References 62 publications

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

110 Years of the Meyer–Overton Rule: Predicting Membrane Permeability of Gases and Other Small Compounds

No facilitator required for membrane transport of hydrogen sulfide

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