Permeability mapping of gelatin methacryloyl hydrogels

Miri, Amir K.; Hosseinabadi, Hossein Goodarzi; Çeçen, Berivan; Hassan, Shabir; Zhang, Yu Shrike

doi:10.1016/j.actbio.2018.07.006

Cited by 83 publications

(71 citation statements)

References 55 publications

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“…However, it should be noted that these permeability values represent those in the bioprinted hollow channels only and not when encapsulated in the hydrogel matrices. The permeability of the latter can be directly calculated using our recently developed model [73] .…”

Section: Permeability Evaluations Of the Bioprinted Hollow Tubesmentioning

confidence: 99%

A Tumor‐on‐a‐Chip System with Bioprinted Blood and Lymphatic Vessel Pair

Cao

Ashfaq

Cheng

et al. 2019

Adv Funct Materials

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139

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Current in vitro anti-tumor drug screening strategies are insufficiently portrayed lacking true perfusion and draining microcirculation systems, which may post significant limitation in reproducing the transport kinetics of cancer therapeutics explicitly. Herein, we report the fabrication of an improved tumor model consisting of bioprinted hollow blood vessel and lymphatic vessel pair, hosted in a threedimensional (3D) tumor microenvironment-mimetic hydrogel matrix, termed as the tumor-on-a-chip with bioprinted blood and lymphatic vessel pair (TOC-BBL). The bioprinted blood vessel was perfusable channel with opening on both ends while the bioprinted lymphatic vessel was blinded on one end, both of which were embedded in a hydrogel tumor mass, with vessel permeability individually tunable through optimization of the composition of the bioinks. We demonstrated that systems with different combinations of these bioprinted blood/lymphatic vessels exhibited varying levels of diffusion profiles for biomolecules and anti-cancer drugs. Our TOC-BBL platform mimicking the natural pathway of drug-tumor interactions would have the drug introduced through the perfusable blood vessel, cross the vascular wall into the tumor tissue via diffusion, and eventually drained into the lymphatic vessel along with the carrier flow. Our results suggested that this unique in vitro tumor model containing the bioprinted blood/lymphatic vessel pair may have the capacity of simulating the complex transport mechanisms of certain pharmaceutical compounds inside the tumor microenvironment, potentially providing improved accuracy in future cancer drug screening.

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Section: Permeability Evaluations Of the Bioprinted Hollow Tubesmentioning

confidence: 99%

A Tumor‐on‐a‐Chip System with Bioprinted Blood and Lymphatic Vessel Pair

Cao

Ashfaq

Cheng

et al. 2019

Adv Funct Materials

Self Cite

139

123

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“…These nanopores are indicative of interchain distances that might be formed by the crosslinked polymer chains within the GelMA hydrogel. [17]…”

Section: Synthesis Of Hierarchically Macro-micro-nanoporous Cell-ladementioning

confidence: 99%

Bioprinted Injectable Hierarchically Porous Gelatin Methacryloyl Hydrogel Constructs with Shape‐Memory Properties

Ying

Jiang

Parra-Cantu

et al. 2020

Adv Funct Materials

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139

124

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Direct injection of cell-laden hydrogels shows high potential for tissue regeneration in translational therapy. The traditional cell-laden hydrogels are often used as bulk space fillers to tissue defects after injection, likely limiting their structural controllability. On the other hand, patterned cell-laden hydrogel constructs often necessitate invasive surgical procedures. To overcome these problems, herein, a unique strategy is reported for encapsulating living human cells in a pore-forming gelatin methacryloyl (GelMA)-based bioink to ultimately produce injectable hierarchically macro-micro-nanoporous cellladen GelMA hydrogel constructs through 3D extrusion bioprinting. The hydrogel constructs can be fabricated into various shapes and sizes that are defect-specific. Due to the hierarchically macro-micro-nanoporous structures, the cell-laden hydrogel constructs can readily recover to their original shapes, and sustain high cell viability, proliferation, spreading, and differentiation after compression and injection. In addition, in vivo studies further reveal that the hydrogel constructs can integrate well with the surrounding host tissues. These findings suggest that the unique 3D-bioprinted pore-forming GelMA hydrogel constructs are promising candidates for applications in minimally invasive tissue regeneration and cell therapy.

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“…When fully crosslinked, a hydrogel with a high DoF will display high stiffness and smaller mesh size. Furthermore, a higher stiffness means that the enzymatic degradation rate by metalloproteinase decreases, which is critical for in vivo applications [46]. An increase in GelMA concentration decreases the mesh size and therefore increases the hydrogel stiffness [47].…”

Section: Materials Characterizationmentioning

confidence: 99%

“…An increase in mesh size increases the diffusion rate, whereas reduced mesh size can slow down the diffusion or entrap the drug in the matrix [33]. Miri et al studied the impact of the DoF and curing conditions on hydrogel stiffness and reported a slower release rate of bovine serum albumin when the GelMA concentration was increased [46]. More precisely, it is the difference between mesh size and drug size that controls drug diffusion rates.…”

Section: Introductionmentioning

confidence: 99%

Gelatin Methacryloyl Hydrogels Control the Localized Delivery of Albumin-Bound Paclitaxel

et al. 2020

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Hydrogels are excellent candidates for the sustained local delivery of anticancer drugs, as they possess tunable physicochemical characteristics that enable to control drug release kinetics and potentially tackle the problem of systemic side effects in traditional chemotherapeutic delivery. Yet, current systems often involve complicated manufacturing or covalent bonding processes that are not compatible with regulatory or market reality. Here, we developed a novel gelatin methacryloyl (GelMA)-based drug delivery system (GelMA-DDS) for the sustained local delivery of paclitaxel-based Abraxane®, for the prevention of local breast cancer recurrence following mastectomy. GelMA-DDS readily encapsulated Abraxane® with a maximum of 96% encapsulation efficiency. The mechanical properties of the hydrogel system were not affected by drug loading. Tuning of the physical properties, by varying GelMA concentration, allowed tailoring of GelMA-DDS mesh size, where decreasing the GelMA concentration provided overall more sustained cumulative release (significant differences between 5%, 10%, and 15%) with a maximum of 75% over three months of release, identified to be released by diffusion. Additionally, enzymatic degradation, which more readily mimics the in vivo situation, followed a near zero-order rate, with a total release of the cargo at various rates (2–14 h) depending on GelMA concentration. Finally, the results demonstrated that Abraxane® delivery from the hydrogel system led to a dose-dependent reduction of viability, metabolic activity, and live-cell density of triple-negative breast cancer cells in vitro. The GelMA-DDS provides a novel and simple approach for the sustained local administration of anti-cancer drugs for breast cancer recurrence.

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Permeability mapping of gelatin methacryloyl hydrogels

Cited by 83 publications

References 55 publications

A Tumor‐on‐a‐Chip System with Bioprinted Blood and Lymphatic Vessel Pair

A Tumor‐on‐a‐Chip System with Bioprinted Blood and Lymphatic Vessel Pair

Bioprinted Injectable Hierarchically Porous Gelatin Methacryloyl Hydrogel Constructs with Shape‐Memory Properties

Gelatin Methacryloyl Hydrogels Control the Localized Delivery of Albumin-Bound Paclitaxel

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